Cell isolation and culture
BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation.
... the isolation of BMhMSC would simplify and enable direct cross-comparison to take place in the absence of distinct ...and isolation is glucose. Cell culture media utilized in the ...
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Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli
... tissues. Cell isolation by tissue dissociation is a major step in cell culture, and many factors can affect tissue dissociation ...viable cell yield, and it can usually be de- termined ...
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Nk Cell Culture Protocol
... applications of CARexpressing natural killer cells, Frontiers in Pharmacology. Would that have caused issues with my lining just sitting there and not shedding all those years? IFNγ levels were quantitated in ...
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Isolation and continuous culture of Neorickettsia helminthoeca in a macrophage cell line
... pathogenic to the canine species, three naive dogs were fed metacercaria-encysted kidneys of salmon caught in a river where infection of metacercariae with Neorickettsia helminthoeca has[r] ...
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Isolation and differentiation of herpes simplex virus and Trichomonas vaginalis in cell culture
... rly (15 to 18 h), it is often possible to reach a in vitro nor the growth of trichomonads on cell monolayers without having to wait for more extensive and in the absence of serum has bee[r] ...
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Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes
... Approximately 10 to 100 50%o tissue culture infective doses of trypsin-treated virus were incubated with 10-fold dilutions of inactivated hyper- immune antisera before inoculation into o[r] ...
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Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
... The choice of exosome marker for immobilization is a key issue. For MHC Class II– positive cells, the use of beads precoated with a pan class II–specific antibody is ideal. However, this marker is unsuitable for exosome ...
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Isolation of the equine granulocytic ehrlichiosis agent, Ehrlichia equi, in tick cell culture
... a cell line, IDE8, derived from a putative vector, the tick Ixodes ...tick cell culture medium with NaHCO 3 and an organic buffer [3-(N- morpholino)-propanesulfonic acid] ...Giemsa-stained ...
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First Isolation of Cytopathogenic Bovine Torovirus in Cell Culture from a Calf with Diarrhea
... Virus isolation in HRT-18 cells. At the second passage, CPE appeared in HRT-18 cells inoculated with the ileum contents of the dead calf. CPE characterized by the enlargement of cells were observed 2 to 3 days ...
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Isolation and culture of grapevine protoplasts
... Isolation methods for protoplasts from different ergans and tissue of grapevine were established. Cell division could not be induced in protoplasts from leaves, shoot tips, p[r] ...
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ISOLATION AND CULTURE OF ACINETOBACTER BAUMANNII
... The present work deals with physiological studies on bacterial L- asparaginase. It includes studies on the productivity of L-asparaginase by different Acinetobacter baumannii is isolated and cell immobilization. ...
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Isolation of an Anaplasma sp Organism from White Tailed Deer by Tick Cell Culture
... tick cell cultures and incubated at 34°C, and 8 days later, Anaplasma-like inclusions were demonstrated in Giemsa-stained culture ...a culture isolate designated WTD76 was verified as the WTD agent ...
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Isolation of common carp ovarian follicular cells and evaluation of their endocrine activity in primary cell culture
... major cell layers; an outer theca layer and an inner granulose (Unal et ...Tissue culture of isolated follicular cells offers an opportunity to examine the ability of granulosa and theca cells to secrete ...
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Isolation, selection and culture methods to enhance clonogenicity of mouse bone marrow derived mesenchymal stromal cell precursors
... activated cell sorting of CD45 − /Ter119 − mBM stroma based on Sca-1 expression and expansion in hypoxic conditions, we show that Sca-1 + cells had higher CFU-F frequencies and showed enhanced proliferation ...
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Use of Shell Vial Cell Culture Assay for Isolation of Bacteria from Clinical Specimens: 13 Years of Experience
... isolates. Based on our experience with the growth of fastidious microorganisms, we have used a centrifugation shell-vial technique called JNSP, for “je ne sais pas” (“I don’t know [what I am growing]”) for the ...
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Isolation of a replication-efficient mutant of West Nile virus from a persistently infected genetically resistant mouse cell culture.
... The amount of interferon produced by resist- ant and susceptible cultures infected with either WNV-103 or WNV-RV (multiplicity of infection of 10) was measured in culture fluids harveste[r] ...
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Advances in crustacean cell culture
... suitable cell culture conditions for the survival and proliferation of various tissues (Toullec, ...crustacean cell line has been established. Development of a cell line provides many ...of ...
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Cell Culture Bioprocess Engineering
... involving cell line isolation, banking, and ...for cell expansion, but new points of view have replaced the notion that “optimal” growth conditions are likely to be found by recreating the native ...
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Isolation and culture of microvascular endothelium from human adipose tissue
... Electron microscopy demonstrated the presence of characteristic tight junctions and pinocytotic vesicles. Immunofluorescent staining for Factor VIII was positive, and cultures contained angiotensin-converting enzyme ...
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Isolation of a cell detaching factor of Trichomonas vaginalis
... i'aginalis in cell culture, and serial twofold dilutions (undiluted to 1:64) were inoculated onto McCoy monolayers in triplicate. 2, each curve represents observations of the monolayers [r] ...
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