Cell Lines and Medium
Human lung-derived mesenchymal stem cell-conditioned medium exerts in vitro antitumor effects in malignant pleural mesothelioma cell lines
... Lourdes Cortes-Dericks * , Laurene Froment, Gregor Kocher and Ralph A. Schmid Abstract Background: The soluble factors secreted by mesenchymal stem cells are thought to either support or inhibit tumor growth. Herein, we ...
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Blood-based lung cancer biomarkers identified through proteomic discovery in cancer tissues, cell lines and conditioned medium
... We have expanded on these approaches, broadening the scope of biomarker discovery by performing prote- omic analyses across multiple types of specimens: freshly resected lung tissues, cancer cell lines and ...
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Human omental adipose-derived mesenchymal stem cell-conditioned medium alters the proteomic profile of epithelial ovarian cancer cell lines in vitro
... O-aDscs-conditioned medium mainly positioning in mitochondria, plays an important role to maintain mitochondria ...cancer cell prolifera- tion, apoptosis, and migration, partially through mediating MMP-2 ...
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Enrichment of Prostate Cancer Stem-Like Cells from Human Prostate Cancer Cell Lines by Culture in Serum-Free Medium and Chemoradiotherapy
... tests, cell in- vasion assays, and tumor xenografts in BALB/c nude mice were used to evaluate the stem cell properties of CD133 + /CD44 + PCa cells that were isolated via fluorescence-activated cell ...
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Cell Lines in vitro
... tumor cell lines, confluent monolayer were treated with trypsin-versene and cells were further dispensed by pipetting in growth medium then ...
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Examples Of Cell Lines
... of cell lines that involved discovering nanobodies produced, especially well as ...that cell line data from ...that cell culture media is critical reviews ...mosquito cell line with a ...
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Chandipura virus growth kinetics in vertebrate cell lines, insect cell lines & embryonated eggs
... It has already been documented earlier that embryonated eggs yield high virus titre than the cell lines 14 . Our study also demonstrated high virus yield in embryonated eggs than vertebrate and invertebrate ...
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Growth and Maintenance of Insect Cell Lines
... your cell lines to the appropriate serum- free ...containing medium in the cell culture. Changing the medium over to serum-free without adaptation can lead to greatly decreased doubling ...
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Adenovirus Types 11p and 35p Show High Binding Efficiencies for Committed Hematopoietic Cell Lines and Are Infective to These Cell Lines
... METHODS Cell lines and culture conditions. The cell lines used, all of human origin, were as follows: Jurkat, an acute T-cell leukemia cell line; DG75, established from a ...
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Cold storage and cryopreservation of tick cell lines
... each cell line, cells harvested by pipetting from 12 to 15 tubes were pooled, and centrifuged at 400 × g for 10 minutes at ...The cell pellet was resuspended in 5 ml of the respective medium and ...
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Breast cancer cell lines: friend or foe?
... organoid medium we used originally [30] with keratinocyte serum-free medium supplemented with bovine pituitary extract and recombinant epidermal growth ...initial cell yield and the ability to ...
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EFFECT OF HETEROSCORPION VENOM ON MCF CELL LINES
... to medium containing varying concentrations of venom (10, 20, 30, 50, and 75 µM/ml), cell viability decreased 62%, 58%, 49%, 41% and 29% respectively, after 24 ...cancer cell lines was ...
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Growth and Maintenance of T-REx Cell Lines
... in medium with FBS that contains tetracycline, you may observe low basal levels of expression from your gene of ...™ cell lines in medium with FBS that contains ...
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Generation of EBV-immortalized B cell lines
... 1. Isolate PBMC using Ficoll method (see UCAN-U 0001) 2. Resuspend 2 x 10 6 cells in 0,25ml 10% FCS culture medium in a 5ml FACS tube. 3. Add 0,25ml EBV supernatant from the B95-8 cell line and incubate for ...
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THE EFFECT OF HYPERTHERMIA ON THE DIFFERENTIATION OF LEUKEMIC CELL LINES
... 1640 medium (Gibco) supplemented with 10% fetal calf serum (prepared freshly in our lab), 120 mg/L penicillin, ,md 200 mg/L ...(Nunc). Cell viability was determined by trypan blue dye exclusion assay and ...
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Modulators of MRP1 promoter in Neuroblastoma cell lines
... LB medium supplemented with the ampicillin antibiotic (50 ...the cell suspensions 250 µl of the Lysis Solution was added and mixed thoroughly by inverting the tubes 4-6 times until the solution became ...
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Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines
... the cell medium and did not monitor the cellular up- take of lycopene, which could be highly variable among cell ...on cell cycle progression in different human cancer cell lines ...
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Single cell states in the estrogen response of breast cancer cell lines
... For instance, the two transcription factors E2F2 and E2F1 are found to peak at state 2 and 5, but are otherwise down-regulated. Marker Genes. We also identified the state-specific marker genes. In each state, genes were ...
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Cell interactions and the response to ecolysteroids of 'Drosophila' imaginal disc cell lines
... tissue culture techniques. This consisted of the use of a special cloning dish which was manufactured from two different % Petri dishes (Fig. 3.12). The plate was made by sticking an 'i inverted 3cm Petri dish base to ...
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LONG LINES NOT SO PRACTICAL IN CHINA WHILE SMALL AND MEDIUM SIZED LINES FAVORED
... Soon, with the construction, much more demonstration work was done to more maglev railroads, e.g., the feasibility demonstration of maglev railroad between Beijing and Ti[r] ...
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