Cell-wall anchored protein
Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram Positive Bacterial Cell Wall Anchored Protein
... X-ray structure of LcpA from Actinomyces oris. To obtain insight into how LcpA glycosylates GspA, we first determined the molecular structure of the LcpA enzyme. An inspection of its primary sequence reveals a tripartite ...
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The Staphylococcus aureus Cell Wall Anchored Protein Clumping Factor A Is an Important T Cell Antigen
... T cell subsets is now considered crucial for next-generation ...T cell antigens of S. aureus. This study highlights the importance of cell wall-anchored pro- teins as human CD4 ⫹ T ...
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Production of a functional cell wall anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
... a cell wall-anchored cel- lulosome opens several potential avenues by which activ- ity could be ...of cell wall attachment could be further ...the protein; the sortase signal may ...
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Development of novel surface display platforms for anchoring heterologous proteins in Saccharomyces cerevisiae
... anchor protein. By directly fusing the target protein to the N-terminus of Aga1p and inserting a flexible linker, the display effi- ciency almost doubled, and the activity of reporter protein ...
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Two Novel, Putatively Cell Wall-Associated and Glycosylphosphatidylinositol-Anchored α-Glucanotransferase Enzymes of Aspergillus niger
... chimeric protein AgtA-SBD (a fusion between AgtA and the starch binding domain [SBD] of ...equivalent protein sequence published by the Department of Energy Joint Genome Institute ...
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Insight into the Antiadhesive Effect of Yeast Wall Protein 1 of Candida albicans
... GPI- anchored proteins may also result from cell wall remodeling or from washout during the membrane-to-glucan transfer process (12, 33, 37, 52, ...the wall and thiol extractable only if the ...
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Mutational Analysis of the Glycosylphosphatidylinositol (GPI) Anchor Pathway Demonstrates that GPI-Anchored Proteins Are Required for Cell Wall Biogenesis and Normal Hyphal Growth in Neurospora crassa
... five cell wall proteins whose amino acid sequences indicate they are secreted without a GPI ...nonanchored cell wall proteins were represented in bands 2, 3, 4, and 8 of ...major ...
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The Glycosylphosphatidylinositol-Anchored DFG Family Is Essential for the Insertion of Galactomannan into the β-(1,3)-Glucan–Chitin Core of the Cell Wall of Aspergillus fumigatus
... the cell wall glucans (17, ...non-GPI cell wall mannoproteins ...GPI-anchored protein, Gas1p, re- mained unchanged in both Dfg5 and Dcw1 single-deletion mutants in ...non-GPI- ...
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A Temperature-Sensitive dcw1 Mutant of Saccharomyces cerevisiae Is Cell Cycle Arrested with Small Buds Which Have Aberrant Cell Walls
... in cell wall biogenesis and to be essential for ...membrane protein. To investigate the roles of these proteins in cell wall biogenesis and cell growth, we constructed mutant ...
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The elastin binding protein of Staphylococcus aureus (EbpS) is expressed at the cell surface as an integral membrane protein and not as a cell wall associated protein
... elastin-binding protein was previously suggested to be a microbial surface protein recognizing adhesive matrix mole- cules ...surface protein MSCRAMMs that promote attachment to collagen, ...
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C-Terminal Signals Regulate Targeting of Glycosylphosphatidylinositol-Anchored Proteins to the Cell Wall or Plasma Membrane in Candida albicans
... fluorescent protein (GFP) to short peptides from the N and C termini of the ...the cell surface, (ii) to identify the site in Hwp1p, and (iii) to assess the effects of specific amino acid substitutions on ...
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Cellulosomics of the cellulolytic thermophile Clostridium clariflavum
... A large set of genes encoding 79 dockerin-containing proteins is present in the C. clariflavum genome; 75 of them have type I dockerins whereas four possess type II dockerins, which, similar to C. thermocellum and A. ...
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AFLMP1 Encodes an Antigenic Cell Wall Protein in Aspergillus flavus
... eukaryotic cell membranes (5, ...the cell membrane into the cell wall, Aflmp1p may fulfill many impor- tant physiological functions, such as cell-cell recognition, cell ...
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Surface display of heterologous proteins in Bacillus thuringiensis using a peptidoglycan hydrolase anchor
... for cell wall binding in the recombinant MB174 cells, but not for the MB173 cells that expressed the cellular WlacD ...the cell cytosol sample ...that cell expressed recombinant enzyme ...
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Structural Insights into Tail-Anchored Protein Targeting by Get3
... A remaining complication in this study is the stoichiometry of the tetramer/TA complex. The current idea is that a single TA protein binds to the Get3 complex. While this is attractive, there is no biochemical ...
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<p>The Antibacterial Activity and Mechanism of Action of Luteolin Against <em>Trueperella pyogenes</em></p>
... T. pyogenes is a Gram-positive, rod-shaped, non-motile, non-spore-forming oppor- tunistic pathogen with a broad range of virulence factors such as pyolisin (PLO), neuraminidases (NanH), collagen-binding protein ...
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Display of a β-mannanase and a chitosanase on the cell surface of Lactobacillus plantarum towards the development of whole-cell biocatalysts
... [3]. Cell wall-anchored CsnA in our study produced ...dry cell weight, using the lipoprotein anchor and the cell wall anchor respectively, obtained are significantly higher than ...
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EPICARDIAL WIRELESS PACEMAKER FOR IMPROVED LEFT VENTRICULAR RESYNCHRONIZATION (CONCEPTUAL DESIGN)
... Cardiac resynchronization therapy attempts to restore the synchronous pacing of both ventricles. First a pacemaker, along with right and left side leads must be implanted. A CRT system consists of a pulse generator and ...
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Role of the Cell Wall Integrity and Filamentous Growth Mitogen-Activated Protein Kinase Pathways in Cell Wall Remodeling during Filamentous Growth
... of cell surface proteins (22) to modulate their adher- ence properties and create cell surface variegation (53, ...fungal cell surface also contribute to the expansion of cells in connected mats of ...
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C.8 Intro Cell Division & Mitosis ppt
... Figure 8.6b-0 Cytokinesis Cell wall of the parent cell Daughter nucleus Cell plate forming Cell wall New cell wall Vesicles containing cell wall material. Cell plate Daughter cells.[r] ...
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