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Cells, Cultured

Pneumocystis jirovecii Can Be Productively Cultured in Differentiated CuFi 8 Airway Cells

Pneumocystis jirovecii Can Be Productively Cultured in Differentiated CuFi 8 Airway Cells

... Immunofluorescence staining of formalin-fixed, paraffin- embedded sections of infected CuFi-8 cells using a monoclonal antibody against P. jirovecii (Dako, Hamburg, Germany) resulted in a strong fluorescent ...

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Activation of cultured vascular endothelial cells by antiphospholipid antibodies

Activation of cultured vascular endothelial cells by antiphospholipid antibodies

... activation of cultured human umbilical vein endothelial cells (HUVEC) by IgG from patients with anticardiolipin antibodies (aCL). Incubation of HUVEC for 4 h with purified IgG (100 micrograms/ml) from ...

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Nutrients Modulate T1r2 Transcript Levels in MIN 6 and Primary Cultured Taste Buds Cells under High Glucose Condition

Nutrients Modulate T1r2 Transcript Levels in MIN 6 and Primary Cultured Taste Buds Cells under High Glucose Condition

... primary cultured taste buds cells using quantitative Reverse Trancription Polymerase Chain ...primary cultured taste buds ...6 cells in the medium with 25 mM ...6 cells in the medium ...

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Metabolism of testosterone 14C by cultured human cells

Metabolism of testosterone 14C by cultured human cells

... by cultured human fibroblasts and amniotic fluid cells was ...the cultured cells for 48 hr, and the labeled metabolites present in the medium were subsequently ...by cultured ...

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Cultured epidermal stem cells in regenerative medicine

Cultured epidermal stem cells in regenerative medicine

... of cultured kerati- nocytes and melanocytes to treat stable vitiligo was first performed in 1992 ...donor cells to produce CES requires a smaller donor biopsy than split-skin grafts, resulting in less ...

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Processing of Alzheimer's amyloid precursor protein in cultured cells

Processing of Alzheimer's amyloid precursor protein in cultured cells

... mice, cultured at birth, show reduced viability and a reduction in neurite ...from cells from APP knock-out mice in which APLP2 is up- regulated in response to neurotoxic assault from ...

257

The growth cycle of poliovirus in cultured cells

The growth cycle of poliovirus in cultured cells

... Details of the procedures used in the assay of virus and plaque-form­ ing cells in monkey kidney cell monolayers have been described previ­ ously (Howes, 1959). The composition of the overlay medium used ...

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Lipoprotein binding to cultured human hepatoma cells

Lipoprotein binding to cultured human hepatoma cells

... G2 cells in lipoprotein-containing medium resulted in complete suppression of LDL receptors but did not affect the remnant ...G2 cells seem to be a suitable model for the study of hepatic receptors for ...

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Effects of edible bird's nest (EBN) on cultured rabbit corneal keratocytes

Effects of edible bird's nest (EBN) on cultured rabbit corneal keratocytes

... intestinal cells using Caco-2 cell line showed the highest proliferation in commercial EBN compared to the unprocessed EBN obtained from four different zones of Peninsular ...

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Characterization of a Previously Unidentified Viral Protein in Porcine Circovirus Type 2-Infected Cells and Its Role in Virus-Induced Apoptosis

Characterization of a Previously Unidentified Viral Protein in Porcine Circovirus Type 2-Infected Cells and Its Role in Virus-Induced Apoptosis

... neighboring cells while evading the host immune system (46) and function by eliminating aberrant cells created by DNA damage or those infected by viral pathogens ...various cultured transformed cell ...

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XuefuZhuyu Tang exerts antitumor effects by inhibiting glioma cell metastasis and invasion via regulating tumor microenvironment

<em>XuefuZhuyu Tang</em> exerts antitumor effects by inhibiting glioma cell metastasis and invasion via regulating tumor microenvironment

... U251 cells were divided into five groups: CNC (cells were cultured with normal saline), TSC (cells were treated with TaohongSiwu Tang [TST]), XSC (cells were treated with XZT), THC ...

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Original Article Capsaicin reduces intrinsic apoptosis induced by hypoxia/reoxygenation in rat alveolar type II cells

Original Article Capsaicin reduces intrinsic apoptosis induced by hypoxia/reoxygenation in rat alveolar type II cells

... (RLE-6TN) cells and to determine the mechanism underlying this ...RLE-6TN cells in a 1% O 2 and 5% CO 2 hypoxic chamber for 24 h, before tr- ansferring it to a 21% O 2 and 5% CO 2 incubator for 24 ...The ...

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Effect of hCG on the morphology of rat epididymal epithelial cells in vitro

Effect of hCG on the morphology of rat epididymal epithelial cells in vitro

... Figure 3. Epididymal epithelial cells cultured without DHT and after stimulation with 12.5 U hCG. The cells form a monolayer. The cytoplasm rich in glycogen and PAS-positive substance (arrow) (A) and ...

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Induced Differentiation of Epithelioid Carcinoma Cell Lines: Evidence for Tumor Cell Quantal Mitosis

Induced Differentiation of Epithelioid Carcinoma Cell Lines: Evidence for Tumor Cell Quantal Mitosis

... undifferentiated cells that continue to proliferate under conditions that restrain normal cells from ...proliferating cells is now recognized to arise from the stem cells of origin of the ...

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Mast cell–derived angiopoietin 1 plays a critical role in the growth of plasma cell tumors

Mast cell–derived angiopoietin 1 plays a critical role in the growth of plasma cell tumors

... mast cells, obtained from C57BL/6 mice (National Cancer Institute), were cultured with 10 ng/ml IL-3 (these are referred to here as BMMCs) or with 100 ng/ml SCF plus 50 ng/ml IL-6 plus 10 ng/ml IL-10 (these ...

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The role of fibroblast growth factors in embryonic limb development

The role of fibroblast growth factors in embryonic limb development

... the cells to produce a positional ...of cultured cells by FGF may provide a model for identifying putative additional ...ridge cells also contain transcripts of genes that code for other ...

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150 kD oxygen regulated protein is expressed in human atherosclerotic plaques and allows mononuclear phagocytes to withstand cellular stress on exposure to hypoxia and modified low density lipoprotein

150 kD oxygen regulated protein is expressed in human atherosclerotic plaques and allows mononuclear phagocytes to withstand cellular stress on exposure to hypoxia and modified low density lipoprotein

... of cultured human aortic smooth muscle cells and mononuclear phagocytes (MPs) to hypoxia (pO 2 z 12–14 torr) induces ORP150 transcripts and production of the an- tigen, whereas incubation with either ...

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Characterization of adipose-derived stem cells from subcutaneous and visceral adipose tissues and their function in breast cancer cells

Characterization of adipose-derived stem cells from subcutaneous and visceral adipose tissues and their function in breast cancer cells

... cancer cells, an indirect co-culture experiment was performed in a transwell ...cancer cells and ASCs were seeded in individual chambers of a transwell system for up to 14 days, where they had no direct ...

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High resolution imaging of TGFΒ3 treated human keratinocyte via a newly developed widefield surface plasmon resonance microscope

High resolution imaging of TGFΒ3 treated human keratinocyte via a newly developed widefield surface plasmon resonance microscope

... Keratinocytes cells using the newly developed Widefield Surface Plasmon Resonance (WSPR) system will be ...HaCaTs cultured on SPR substrate with Trans- forming Growth Factor β3 (TGFβ3) (50ng/ml) [2] and ...

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Hyaluronic acid affects the in vitro induction effects of Synthetic PAMPS and PDMAAm hydrogels on chondrogenic differentiation of ATDC5 cells, depending on the level of concentration

Hyaluronic acid affects the in vitro induction effects of Synthetic PAMPS and PDMAAm hydrogels on chondrogenic differentiation of ATDC5 cells, depending on the level of concentration

... ATDC5 cells were significantly affected by HA, depending on the level of con- ...ATDC5 cells cultured on a polystyrene dish does not induce chondrogenic differen- ...the cells which had ...

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