Chimeric virus-like particles
Broad Cross-Protection Is Induced in Preclinical Models by a Human Papillomavirus Vaccine Composed of L1/L2 Chimeric Virus-Like Particles
... epitope (RG1 epitope) (30–32), has been incorporated into the capsid surface DE loop of HPV-16 L1 or HPV-18 L1 to create chimeric L1/L2 VLP vaccines (24, 25, 27). Both L1/L2 vaccines (based on either HPV-16 or ...
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Genetic Mapping of a Highly Variable Norovirus GII.4 Blockade Epitope: Potential Role in Escape from Human Herd Immunity
... created chimeric virus-like particles (VLPs) between the ...blocked chimeric VLP-HBGA interaction, while an ...block chimeric VLP-HBGA interaction and found that epitope A amino ...
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RNase Resistant Virus Like Particles Containing Long Chimeric RNA Sequences Produced by Two Plasmid Coexpression System
... from SARS-CoV; GenBank accession no. AY864806), a pac site (19 bp), HCV (nt 18 to 310, 293 bp from HCV 5⬘UTR; GenBank accession no. AF139594), and HA300 (nt 295 to 611, 317 bp from H5N1 avian influenza virus; ...
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Lack of Interference with Immunogenicity of a Chimeric Alphavirus Replicon Particle-Based Influenza Vaccine by Preexisting Antivector Immunity
... con particles, although the effects of antivector immunity were not directly addressed ...replicon particles may be applicable to humans for multiple administrations with- out decreasing ...influenza ...
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Incorporation of Glycosylphosphatidylinositol-Anchored Granulocyte- Macrophage Colony-Stimulating Factor or CD40 Ligand Enhances Immunogenicity of Chimeric Simian Immunodeficiency Virus-Like Particles
... CD40L chimeric SIV VLPs were very efficient in stimulating robust CD4 ⫹ - and CD8 ⫹ -T-cell responses. Our findings on the induction of T-cell responses by CD40L are consistent with previously published studies ...
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Chimeric murine polyomavirus virus-like particles induce Plasmodium antigen-specific CD8+ T cell and antibody responses
... FIGURE 3 | Cellular immune responses induced by individual (30 µg) or pooled (10 µg each) chimeric VLP immunizations. BALB/c mice (n = 5/group) were immunized with a prime/boost regimen using two i.m., priming ...
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Formation of Wild-Type and Chimeric Influenza Virus-Like Particles following Simultaneous Expression of Only Four Structural Proteins
... of chimeric influenza VLPs bearing the VSV-G ...influenza virus HA, in addition to the three structural influenza virus genes M1, NA, and M2 ...influenza virus proteins M1 and M2 were ...
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Chimeric Hepatitis B core antigen virus-like particles displaying the envelope domain III of dengue virus type 2
... produce chimeric nanoparticles using a fusion antigen compris- ing of the HBcAg polypeptide with EDIII-2 inserted into its surface-exposed c/e1 ...the chimeric nanoparticle and is able to induce the produc- ...
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Incorporation of High Levels of Chimeric Human Immunodeficiency Virus Envelope Glycoproteins into Virus-Like Particles
... of chimeric HIV-1 Env proteins with heter- ologous SP, TM, or CT sequences individually or in combina- tion for their effects on Env incorporation into ...of chimeric HIV-1 Con-S ⌬ CFI Env and resulted in ...
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Virus-Like Particles: Applications in Reverse Vaccinology
... a chimeric and recombinant VLP (rVLPs) made of the circumsporozoite protein of Plasmodium falciparum and the surface antigen of hepatitis B ...mosaic virus, the HPV L1 protein or the bacteriophage Qb, has ...
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An HPV 16 L1 based chimeric human papilloma virus like particles containing a string of epitopes produced in plants is able to elicit humoral and cytotoxic T cell activity in mice
... Parenteral vaccines, such as that for HPV, are expensive to produce and costly to administer. Thus, many diseases that are controlled effectively in industrialized countries are controlled poorly at best in poor ...
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Chimeric HBcAg virus-like particles presenting a HPV 16 E7 epitope significantly suppressed tumor progression through preventive or therapeutic immunization in a TC-1-grafted mouse model
... To assess the potential of VLP-based vaccines to inter- vene with the growth of established tumors, treatment studies were performed. Mice were inoculated with TC-1 cells, and immunization with the vaccine was initiated ...
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Production and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidates
... influenza virus, BALB/c mice were immunized 3 times with chimeric LAH3-HBc VLPs at two weeks ...Thus chimeric LAH3-HBc VLPs induced broadly reactive anti-influenza serum against both group 1 and 2 HA ...
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Chimeric L1-L2 Virus-Like Particles as Potential Broad-Spectrum Human Papillomavirus Vaccines
... of chimeric fusion proteins (by Western blotting of Sf-9 cell lysates infected by recombinant ...of chimeric VLP as major bands within a range of 45 to 60 ...nucleopolyhedrosis virus ...
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Chimeric recombinant rotavirus like particles as a vehicle for the display of heterologous epitopes
... vaccinia virus, the trimerization domain could be delimited to a region between amino-acids 246 and 314 [30], and the N terminal end of this domain was postulated between amino-acids 147 and ...
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Plasmid-Driven Formation of Influenza Virus-Like Particles
... influenza virus proteins PB2, PB1, PA, and NP leads to replication and transcription of an artificial ...influenza virus RNAs ...influenza virus RNA ...influenza virus-like RNAs (12). ...
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Enhancement of Mucosal Immunization with Virus-Like Particles of Simian Immunodeficiency Virus
... NALT, like the gut-associated tissue, can stimu- late the common mucosal immune system, in which immune cells stimulated at one site migrate to other sites through the systemic (blood and lymph) circulation (13, ...
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Structural Requirements for the Assembly of Norwalk Virus-Like Particles
... of particles observed by electron microscopy when the particles were purified over iodixanol or sucrose ...38-nm particles were observed only when particles were purified over iodixanol ...
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Chimeric hepatitis B virus core particles as probes for studying peptide-integrin interactions.
... the virus to cell ...FM.e1-HBc particles were easily purified from ...the virus, it includes a highly conserved triplet, ...the virus via an infectious clone (19, 23) have shown that the RGD ...
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Requirements for Budding of Paramyxovirus Simian Virus 5 Virus-Like Particles
... For immunoprecipitation analysis, cell lysates or sucrose gradient fractions were combined with an equal volume of 2⫻ radioimmunoprecipitation assay (RIPA) buffer (20 mM Tris, pH 7.4; 2% deoxycholate; 2% Triton X-100; ...
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