Chloramphenicol acetyltransferase (CAT)
Chloramphenicol acetyltransferase as a selection marker for chlamydial transformation
... these methods were established with plasmids carrying a β- lactamase, which degrades penicillin family antibiotics, and thus relieves their inhibitory effects of these drugs on the cytokinesis of RBs [8,9]. Since ...
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A poliovirus replicon containing the chloramphenicol acetyltransferase gene can be used to study the replication and encapsidation of poliovirus RNA.
... B A CAT-specific protein with an Mr of 51,000 CAT-VP3 fusion indicated by the arrow is present in extracts of cells transfected with FLC/REP RNA lane 3 but is absent from mock-transfecte[r] ...
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Rescue of a synthetic chloramphenicol acetyltransferase RNA into influenza virus-like particles obtained from recombinant plasmids.
... synthetic CAT RNA and that express all influenza virus-encoded proteins from recombinant plasmids can transmit the synthetic RNA to MDCK ...the CAT RNA (i) depended on trypsin treatment of the culture ...
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Negative regulatory element associated with potentially functional promoter and enhancer elements in the long terminal repeats of endogenous murine leukemia virus-related proviral sequences.
... 2746 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Three series of recombinant DNA clones were constructed, with the bacterial chloramphenicol acetyltransferase CAT g[r] ...
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Localization of sequences responsible for trans-activation of the equine infectious anemia virus long terminal repeat.
... 120 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We used the Escherichia coli chloramphenicol acetyltransferase gene cat to study sequences that influence expression[r] ...
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Inhibition of calcitriol receptor binding to vitamin D response elements by uremic toxins
... Abbreviations used in this paper: CAT, chloramphenicol acetyltransferase; EMSA, electrophoretic mobility shift assay; GH, growth hormone; Nm..,, maximal binding capacity; N-UF, normal ul[r] ...
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Induction of CD4 expression and human immunodeficiency virus type 1 replication by mutants of the interferon-inducible protein kinase PKR.
... To further substantiate the activation of NF-kB by PKRLS9, the chloramphenicol acetyltransferase CAT reporter gene under the control of the wt HIV-1 LTR ptzIIICAT [6] or a mutant form of[r] ...
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Inhibition of TGF alpha gene expression by vitamin A in airway epithelium
... gene chloramphenicol acetyltransferase ...of CAT activity was observed ...inol, CAT activity was reduced by more than ...relative CAT activity when compared with the control ...
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The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure.
... Using constructs which direct synthesis of RNA containing a chloramphenicol acetyltransferase CAT gene between mutagenized influenza virus noncoding sequences derived from the NS genome [r] ...
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Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization.
... GB viruses A and C (GBV-A and GBV-C) are two recently described RNA viruses which appear to be members of the Flaviviridae. Although both viruses appear to contain long 5 * nontranslated regions, the sites of polyprotein ...
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Identification of the hepatitis B virus factor that inhibits expression of the beta interferon gene.
... The chloramphenicol acetyltransferase CAT-containing plasmids that were used in this study were i pTWU54, in which the cat gene is under the control of the 353-bp HindIll-HincIl DNA regi[r] ...
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In Vivo Effects of Mutations in Woodchuck Hepatitis Virus Enhancer II
... a chloramphenicol acetyltransferase (CAT) gene whose expression is dependent upon EnII, mutations in either the HNF-1 or the HNF-4 site strongly reduced CAT activity, while ablation of the ...
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Identification of a transactivating function mapping to the putative immediate-early locus of human herpesvirus 6.
... Effector plasmids containing these HHV-6 coding and 5' sequences were able to effect activation of heterologous promoter-chloramphenicol acetyltransferase CAT constructs, including adeno[r] ...
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17 beta Estradiol inhibits expression of human interleukin 6 promoter reporter constructs by a receptor dependent mechanism
... of chloramphenicol acetyltransferase (CAT) reporter plasmid driven by the human IL-6 promoter was studied here in HeLa or murine bone marrow stromal cells (MBA ...stimulated CAT expression in ...
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The Replication Activity of Influenza Virus Polymerase Is Linked to the Capacity of the PA Subunit To Induce Proteolysis
... Recombinant plasmids encoding the influenza virus polymerase and NP pro- teins (pGPB1, pGPB2, pGPA, pGNP, and pGNPpA) have been described (26, 29). A plasmid-expressing ribozyme construct that originates in vivo a vNSCAT ...
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A reporter transgene indicates renal specific induction of tumor necrosis factor (TNF) by shiga like toxin Possible involvement of TNF in hemolytic uremic syndrome
... a chloramphenicol acetyltransferase (CAT) reporter gene that indicates TNF synthesis, CAT activity was induced within the kidney, but not in other ...
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Influenza virus NS1 protein stimulates translation of the M1 protein.
... M-CAT RNA, which contains the chloramphenicol acetyltransferase CAT reporter gene and the terminal noncoding sequence of segment 7 coding for the Ml and M2 proteins, was ribonucleoprotei[r] ...
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Gattner, Michael (2019): Incorporation of pyrrolysine derivatives into proteins and development of bioorthogonal protein modification methods. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Supporting Figure 2: Biosynthetic conversion of 3 S -ethynyl- D -ornithine to ethynylpyrrolysine and incorporation into chloramphenicol acetyltransferase (CAT) using E..[r] ...
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Identification of upstream promoter elements mediating early transcription from the 35,000-molecular-weight protein gene of Autographa californica nuclear polyhedrosis virus.
... The promoter fragment, extending from positions -226 to +12 relative to the early RNA start site position +1, was fused to the reporter gene encoding chloramphenicol acetyltransferase CA[r] ...
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Tumor necrosis factor alpha and interferon gamma suppress the activation of human type I collagen gene expression by transforming growth factor beta 1 Evidence for two distinct mechanisms of inhibition at the transcriptional and posttranscriptional levels
... (chloramphenicol acetyltransferase) construct, while posttranscriptional regulation was assessed by parallel determinations of type I procollagen mRNA steady-state ...
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