CHO-K1 cells
N- and 6-O-Sulfated Heparan Sulfates Mediate Internalization of Coxsackievirus B3 Variant PD into CHO-K1 Cells
... infection, cells were treated with ammonium chlo- ride, a weak base which increases the pH of endosomes and ...in CHO-K1 but not in pgsD-677-hCAR cells ...HeLa cells expressing CAR and ...
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Transfecting CHO-K1 Cells: Comparison of CaPO4, Electroporation and Lipoplex Method with In-house Prepared Polyplex
... assay. CHO-K1 cell line was procured from National Centre For Cell Science (NCCS), Pune, India and maintained in DMEM medium containing 10% FBS at 37° in 5% CO 2 .... CHO-K1 cells were ...
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Introduction of human genomic sequences renders CHO-K1 cells susceptible to infection by amphotropic retroviruses.
... The 1-gal titer of this ecotropic vector was similar to that of the PA317/LBgSN-conditioned medium when tested on NIH Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest To [r] ...
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Equine Herpesvirus 1 Entry via Endocytosis Is Facilitated by αV Integrins and an RSD Motif in Glycoprotein D
... Hep-2 cells could be observed by using an RGD peptide or MAbs to the human  1 or  4 integrin subunit ...in CHO-K1 ...Vero cells and CHO-K1 cells expressing human ␣ V  3 ...
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Investigation of triamterene as an inhibitor of the TGR5 receptor: identification in cells and animals
... ovary cells (CHO-K1 cells) to express ...transfected cells. In addition, NCI-H716 cells that secrete incretin were also ...
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The Murine Homolog (Mph) of Human Herpesvirus Entry Protein B (HveB) Mediates Entry of Pseudorabies Virus but Not Herpes Simplex Virus Types 1 and 2
... these cells were transfected with the mHveB-expressing and control plasmids to test the alphaherpesvirus entry activity of ...transfected cells were inoculated with recombi- nants of HSV-1(KOS) (21), a ...
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Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells
... The cells did not reach confluence and were distributed in multiple layers inside the porous surface ...in cells with osteogenic lineage on rough Ti surfaces compared with on smooth surfaces [6, ...for ...
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The Ig-Like V-Type Domain of Paired Ig-Like Type 2 Receptor Alpha Is Critical for Herpes Simplex Virus Type 1-Mediated Membrane Fusion
... CELISA, CHO-K1 cells were transfected in 96- well plates with PILR  L139W and ...The cells were washed, incubated with an anti-FLAG M2 antibody, and washed extensively prior to fixation and ...
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A Role for Herpesvirus Entry Mediator as the Receptor for Herpes Simplex Virus 1 Entry into Primary Human Trabecular Meshwork Cells
... TM cells was compared to entry into naturally susceptible Vero cells and CHO-K1 cells expressing either HVEM or 3-OS HS as gD ...of cells (20,000 cells per well, per ...
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Ovine Herpesvirus 2 Glycoproteins B, H, and L Are Sufficient for, and Viral Glycoprotein Ov8 Can Enhance, Cell-Cell Membrane Fusion
... MDBK cells is an important future ...of CHO-K1 cells transfected with the plasmid encoding Ov8 alone (Ov8), with plasmids encoding gB, gH/gL and Ov8 together [Ov8(All)], and the empty vector ...
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NFAT5 is Regulated by p53/miR-27a Signal Axis and Promotes Mouse Ovarian Granulosa Cells Proliferation
... in CHO-K1 ...into CHO-K1 cells together with the pmirGLO-NFAT5 and pmirGLO-NFAT5-mut ...after CHO-K1 cells were transfected with mimics, mimics NC, inhibitor and ...
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Coexpression of ATP binding cassette proteins ABCG5 and ABCG8 permits their transport to the apical surface
... In cells expressing ABCG5-myc or ABCG8-HA alone, the higher–molecu- lar weight forms of the proteins were sensitive to PNGase F, which removes all N-linked sugars, and to Endo H, which removes high-mannose sugars ...
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Herpes B Virus Utilizes Human Nectin-1 but Not HVEM or PILRα for Cell-Cell Fusion and Virus Entry
... target CHO K1 cells with a plasmid encoding luciferase under a T7 promoter and either empty vector, nectin-1, AGM HVEM, or human ...Effector CHO-K1 cells were transfected with a ...
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Expression and Characterization of a Minimal Hepatitis C Virus Glycoprotein E2 Core Domain That Retains CD81 Binding
... into CHO-K1 ...into CHO-K1 ...into CHO-K1 cells by anti-CD81 MAb ...transfected cells, followed by a constant amount of E2 661 myc proteins for 4 h ...
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Cellular Transcription Factor ZASC1 Regulates Murine Leukemia Virus Transcription
... from CHO-K1 and IM1 cells performed with rabbit polyclonal antibodies against human ZASC1 or human actin ( ␣ -ZASC1 and ␣ -actin, ...(C) CHO-K1 and IM1 cells engineered to ...
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Heparan Sulfate Glycosaminoglycans Are Receptors Sufficient To Mediate the Initial Binding of Adenovirus Types 2 and 5
... CAR-negative CHO K1 cells, (ii) binding and infection of CAR-negative CHO K1 cells are inhibited by heparin; and (iii) CAR-negative CHO clones defective in HS GAGs are ...
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Establishment a CHO Cell Line Expressing Human CD52 Molecule
... The cells were collected by treatment with 1% trypsin–EDTA (Bio-IDEA, Iran) and washed twice with PBS, then stained with fluorescein isothiocyanate (FITC)-conjugated mouse anti- human CD52 antibody (Biolegend, ...
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Investigation of Clade B New World Arenavirus Tropism by Using Chimeric GP1 Proteins
... CEM cells and the ability to respond to the pres- ence of hTfR1 transfected into CHO-K1 cells [CHO-K1 (hTfR1)] are properties of the GPs from pathogenic viruses JUNV, MACV, and ...
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Mutations That Affect the Tropism of DA and GDVII Strains of Theiler's Virus In Vitro Influence Sialic Acid Binding and Pathogenicity
... on CHO-K1 ...of CHO-K1 cells by parental and adapted ...to CHO-K1 cells notably mapped to the well-characterized VP1 CD and VP2 EF loops of the ...to ...
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A Role for 3-O-Sulfated Heparan Sulfate in Promoting Human Cytomegalovirus Infection in Human Iris Cells
... HIS cells considered “effector” cells were first infected with HCMV at an MOI of 10 for 7 days followed by transfection with expression plasmid for T7 ...parallel, CHO-K1 cells which ...
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