cholera toxin B subunit
Kinetics of Local and Systemic Immune Responses after Vaginal Immunization with Recombinant Cholera Toxin B Subunit in Humans
... recombinant cholera toxin B subunit (CTB) in generating mucosal and systemic immune responses in healthy women were evaluated, and the kinetics of the immune responses were monitored for ...
6
A Cholera Toxin B-subunit Variant That Binds Ganglioside G M1 but Fails to Induce Toxicity
... of Toxin to Non-polarized T84 Cells—Binding isotherms for wild-type CTB and mutant CTB-H57A were determined by incubating at 4 °C increasing amount of toxins in HBSS and ...the cholera toxin ...
9
Anti-inflammatory effect of cholera toxin B subunit in experimental stroke
... Cholera toxin is composed of one A subunit and five B ...The cholera toxin B subunit (CTB) is devoid of toxicity and capable of ...
14
Comparison of Serum Humoral Responses Induced by Oral Immunization with the Hepatitis B Virus Core Antigen and the Cholera Toxin B Subunit
... that cholera toxin especially induces a Th2 immune response asso- ciated with high titers of IgG1 isotype ...CTxB subunit are used to induce peripheral tolerance to the coupled antigen, an ...
7
Gas phase characterization of the noncovalent quaternary structure of Cholera toxin and the Cholera toxin B subunit pentamer
... CTx B pentamer, the additional interactions present in the CTx B helical region (residues 1–14) may provide the increase in structural stability observed in both the gas and aqueous ...different B 5 ...
10
Broadly protective immunity against divergent influenza viruses by oral co-administration of Lactococcus lactis expressing nucleoprotein adjuvanted with cholera toxin B subunit in mice
... Background: Current influenza vaccines need to be annually reformulated to well match the predicated circulating strains. Thus, it is critical for developing a novel universal influenza vaccine that would be able to ...
10
Influence of Exogenous Reproductive Hormones on Specific Antibody Production in Genital Secretions after Vaginal Vaccination with Recombinant Cholera Toxin B Subunit in Humans
... weekly vaginal vaccinations (16). The progesterone level is low on day 10 and high on day 24 of the cycle, whereas the estrogen level is high at both time points. Kozlowski et al. (19) did evaluate the outcome of monthly ...
6
Cholera Toxin B-Subunit Gene Enhances Mucosal Immunoglobulin A, Th1-Type, and CD8+ Cytotoxic Responses When Coadministered Intradermally with a DNA Vaccine
... CT B subunit was detected with a rabbit anti-CT antibody (Sigma, ...the B subunit of CT (Sigma), by enzyme-linked immunosorbent assay ...
9
A protein-based pentavalent inhibitor of the cholera toxin B-subunit.
... Sedimentation velocity AUC was used to further study the oligomerization state of the protein complexes being formed by ligand binding. At a ratio of 1:1 CTB and W88E(GM1), AUC showed two peaks with sedimentation ...
5
PCR-MEDIATED CLONING A ND EXPRESSION OF THE GENE FOR THE B-SUBUNIT OF VIBRIO CHOLERAE TOXIN ISOLATED RECENTLY IN IRAN
... the cholera toxin operon, we designed oligonucleotide primers for its-PCR amplification from local clinical isolates of ...the cholera toxin B subunit (CTB) was reamplified and cloned ...
6
Designing, Optimization and Construction of Myelin Basic Protein Coding Sequence Binding to the Immunogenic Subunit of Cholera Toxin
... Background and Objectives: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease. Mucosal feeding of myelin basic protein binding to the cholera toxin B subunit can reduce the ...
7
Compound DNA vaccine encoding SAG1/ SAG3 with A2/B subunit of cholera toxin as a genetic adjuvant protects BALB/c mice against Toxoplasma gondii
... In order to evaluate of protection potency, highly viru- lent RH strain of T. gondii was used for the challenge study. No effective vaccine has been shown to com- pletely protect against intraperitoneal challenge with ...
8
Evaluation of Oral Immunization with Recombinant Avian Influenza Virus HA1 Displayed on the Lactococcus lactis Surface and Combined with the Mucosal Adjuvant Cholera Toxin Subunit B
... nontoxic B sub- unit of cholera toxin (CTB), which is a strong mucosal adju- vant, are regarded as the best way to improve immune effi- ciency (2, ...
6
Cell propagation of Cholera Toxin CTA ADP-ribosylating factor by exosome mediated transfer.
... century, cholera remains an epidemic or endemic disease in many parts of the ...the Cholera Toxin (CT) ...and B [2] in that similar to other members of the AB5 family of toxins, that once ...
21
Genetic Determinants Differences between Vibrio cholerae Biotypes
... the cholera in humans. The present cholera global pandemic is attributed to a change among seventh pandemic strains and emergence of ...V. cholera O1 El Tor with altered cholera toxin ...
5
Intranasal Vaccination Using Interleukin-12 and Cholera Toxin Subunit B as Adjuvants To Enhance Mucosal and Systemic Immunity to Human Immunodeficiency Virus Type 1 Glycoproteins
... We have investigated the induction of protective mucosal immunity to human immunodeficiency virus type 1 (HIV-1) isolate 89.6 by intranasal (i.n.) immunization of mice with gp120 and gp140 together with interleu- kin-12 ...
9
Lipopolysaccharide- and Cholera Toxin-Specific Subclass Distribution of B-Cell Responses in Cholera
... with cholera patients have shown that specific ASC peak at around 7 days after the onset of cholera (26, 28), we studied the ASC response at this time point as well as at the acute stage at day ...CT ...
7
Protection against Shiga Toxin-Producing Escherichia coli Infection by Transcutaneous Immunization with Shiga Toxin Subunit B
... as cholera toxin or LT or its mutant derivatives (LTK63 and LTR72), triggers protective immune responses against the toxin and the coadministered antigen (8, 10, ...
8
Development of a Cholera Toxin CTA2/B Based Staphylococcus aureus Vaccine to Prevent Bovine Mastitis
... labile toxin (LTI, LTIIa, LTIIb) and shiga toxin (Stx1 and Stx2), have long been studied as vaccine ...A subunit (240 amino acids; MW 28 kD) located centrally, and five homomeric B subunits ...
177
Changing epidemiology and antimicrobial resistance in Vibrio cholerae: AMR surveillance findings (2006–2016) from Nepal
... Cholera is an acute diarrheal illness caused by the bac- terium Vibrio cholerae [1]. Until now, seven distinct pandemics of cholera have been recorded [2]. The earlier pandemics were caused by the classical ...
8