cholera toxin subunit B
Evaluation of Oral Immunization with Recombinant Avian Influenza Virus HA1 Displayed on the Lactococcus lactis Surface and Combined with the Mucosal Adjuvant Cholera Toxin Subunit B
... The development of safe and efficient avian influenza vaccines for human and animal uses is essential for preventing virulent outbreaks and pandemics worldwide. In this study, we constructed a recombinant (pgsA- HA1 gene ...
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Intranasal Vaccination Using Interleukin-12 and Cholera Toxin Subunit B as Adjuvants To Enhance Mucosal and Systemic Immunity to Human Immunodeficiency Virus Type 1 Glycoproteins
... We have investigated the induction of protective mucosal immunity to human immunodeficiency virus type 1 (HIV-1) isolate 89.6 by intranasal (i.n.) immunization of mice with gp120 and gp140 together with interleu- kin-12 ...
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Genetic Determinants Differences between Vibrio cholerae Biotypes
... the cholera in humans. The present cholera global pandemic is attributed to a change among seventh pandemic strains and emergence of ...V. cholera O1 El Tor with altered cholera toxin ...
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Influence of Exogenous Reproductive Hormones on Specific Antibody Production in Genital Secretions after Vaginal Vaccination with Recombinant Cholera Toxin B Subunit in Humans
... The objective of this study was to investigate the influence of exogenous reproductive hormones on the local and systemic production of specific immunoglobulin A (IgA) and IgG antibodies after vaginal vaccination with ...
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Gas phase characterization of the noncovalent quaternary structure of Cholera toxin and the Cholera toxin B subunit pentamer
... CTx B pentamer, the additional interactions present in the CTx B helical region (residues 1–14) may provide the increase in structural stability observed in both the gas and aqueous ...different B 5 ...
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Lipopolysaccharide- and Cholera Toxin-Specific Subclass Distribution of B-Cell Responses in Cholera
... with cholera patients have shown that specific ASC peak at around 7 days after the onset of cholera (26, 28), we studied the ASC response at this time point as well as at the acute stage at day ...CT ...
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Protection against Shiga Toxin-Producing Escherichia coli Infection by Transcutaneous Immunization with Shiga Toxin Subunit B
... as cholera toxin or LT or its mutant derivatives (LTK63 and LTR72), triggers protective immune responses against the toxin and the coadministered antigen (8, 10, ...
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Development of a Cholera Toxin CTA2/B Based Staphylococcus aureus Vaccine to Prevent Bovine Mastitis
... labile toxin (LTI, LTIIa, LTIIb) and shiga toxin (Stx1 and Stx2), have long been studied as vaccine ...A subunit (240 amino acids; MW 28 kD) located centrally, and five homomeric B subunits ...
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PCR-MEDIATED CLONING A ND EXPRESSION OF THE GENE FOR THE B-SUBUNIT OF VIBRIO CHOLERAE TOXIN ISOLATED RECENTLY IN IRAN
... Knowing the nucleotide sequence of the cholera toxin gene CtxAb, 36,31 we designed a pair of oligonucleotide primers that annealed to opposite ends'of the CT -coding region. The 5'-terminal ( forward) ...
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Comparison of Serum Humoral Responses Induced by Oral Immunization with the Hepatitis B Virus Core Antigen and the Cholera Toxin B Subunit
... cells, B cells, macrophages, and dendritic ...of B cells in antigen presentation and concom- itant induction of an immune response following HBc admin- istration in an in vivo mouse ...T-cell-independent ...
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Compound DNA vaccine encoding SAG1/ SAG3 with A2/B subunit of cholera toxin as a genetic adjuvant protects BALB/c mice against Toxoplasma gondii
... The compound gene was obtained by T-A cloning (TaKaRa, Dalian) and introduced into the eukaryotic ex- pression plasmid pcDNA3.1 (−) vector by EcoR I/ BamH I, EcoR I/Kpn I or EcoR I/Hind III cloning sites separately. The ...
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Cell propagation of Cholera Toxin CTA ADP-ribosylating factor by exosome mediated transfer.
... century, cholera remains an epidemic or endemic disease in many parts of the ...the Cholera Toxin (CT) ...and B [2] in that similar to other members of the AB5 family of toxins, that once ...
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Broadly protective immunity against divergent influenza viruses by oral co-administration of Lactococcus lactis expressing nucleoprotein adjuvanted with cholera toxin B subunit in mice
... Background: Current influenza vaccines need to be annually reformulated to well match the predicated circulating strains. Thus, it is critical for developing a novel universal influenza vaccine that would be able to ...
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A Cholera Toxin B-subunit Variant That Binds Ganglioside G M1 but Fails to Induce Toxicity
... wt toxin to elicit maximal binding and a maximal physiologic response), still ⬃ 30 – 40% less CTB-H57A was bound to T84 cell ...associated toxin (data shown below in ...
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Anti-inflammatory effect of cholera toxin B subunit in experimental stroke
... Cholera toxin is composed of one A subunit and five B ...The cholera toxin B subunit (CTB) is devoid of toxicity and capable of ...
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Cholera Toxin B-Subunit Gene Enhances Mucosal Immunoglobulin A, Th1-Type, and CD8+ Cytotoxic Responses When Coadministered Intradermally with a DNA Vaccine
... enterotoxin B-subunit mature polypeptide was amplified with the following oli- gonucleotides: CtBF (5⬘-TCA GGC GGC CGC CAT ATG CAC ATG AGG CAC CT-3⬘) and CtBR (5⬘-TCA GTC TAG ATT AAT TTG CCA TAC TAA TTG ...
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Designing, Optimization and Construction of Myelin Basic Protein Coding Sequence Binding to the Immunogenic Subunit of Cholera Toxin
... Background and Objectives: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease. Mucosal feeding of myelin basic protein binding to the cholera toxin B subunit can reduce the ...
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A protein-based pentavalent inhibitor of the cholera toxin B-subunit.
... Here we report a multivalent inhibitor for cholera toxin, based on an inactive mutant CTB protein that is modified with GM1os ligands (Figure 1). As the majority of the structure comes as a ready-made ...
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Kinetics of Local and Systemic Immune Responses after Vaginal Immunization with Recombinant Cholera Toxin B Subunit in Humans
... The present study shows that two vaginal vaccinations were required for the induction of strong IgA and IgG antitoxin responses in the human genital tract. None of the vaccinees responded with IgA antitoxin titer ...
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Studies on the Mode of Action of Cholera Toxin: EFFECTS ON SOLUBILIZED ADENYLATE CYCLASE
... and toxin-treated rat livers were ...of cholera toxin. Cholera toxin caused a ...of cholera toxin to stimulate adenylate cyclase is present even when the enzyme membrane ...
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