cis-acting DNA elements
Lentiviral vector design using alternative RNA export elements
... various cis-acting DNA elements that co-ordinate RNA export during viral production to determine their ability to affect the efficiency of vector titer and transduction in different ...
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Characterization of cis-acting elements of NtPMT3 and trans-acting factors of NtQPT1 in Nicotiana tabacum
... response elements (AuxREs) found in promoters of primary/early auxin response genes (Ulmasov et ...amino-terminal DNA binding domain and a carboxyl-terminal dimerization domain related to domains III and IV ...
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Minimal cis-Acting Elements Required for Adenovirus Genome Packaging
... adequate for packaging. In the experiments described thus far, the right-end ITR and its covalently linked terminal protein were still present in the Ad(ITR:HO:⌿) genomes cleaved at the left end by HO endonuclease. A ...
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cis- and trans-Acting Elements in Flavivirus RNA Replication
... FIG. 6. Characterization of secreted complemented viruses by RT-PCR analysis. (A) Schematic representation of the KUN full-length (wild-type [wt]) and replicon genomes and predicted sizes of the RT-PCR products. The num- ...
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Identification of cis - and trans -acting elements regulating calretinin expression in mesothelioma cells
... for DNA methylation in regulating calretinin ...a DNA hypomethylating agent, did not increase calretinin expression in low-calretinin expressing SPC111 ...altered DNA methylation of the CALB2 ...
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cis-Acting Elements Important for Retroviral RNA Packaging Specificity
... the protocol illustrated in Fig. 3A. These vectors were intro- duced into helper cells expressing MLV Gag/Gag-Pol (PG13) or SNV Gag/Gag-Pol (DSH134G) by DNA transfection. Hy- gromycin-resistant colonies from each ...
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Characterization of cis-Acting and NS1 Protein-Responsive Elements in the p6 Promoter of Parvovirus B19
... sequence elements abso- lutely necessary for the transactivator function of the NS1 protein obviously lie within the region spanning nt 100 to 160 of the p6 promoter ...and DNA could not be shown for ...
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The Gene Product of Human Cytomegalovirus Open Reading Frame UL56 Binds the pac Motif and Has Specific Nuclease Activity
... the cis-acting human cytomegalovirus (HCMV) packaging elements (pac 1 and pac 2) as DNA probes, specific DNA-protein complexes were detected by electrophoretic mobility shift assay ...
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cis-acting elements in the lytic origin of DNA replication of Epstein-Barr virus.
... The efficiency of lytic-cycle DNA replication is influenced by sequence elements flanking the core of the lytic origin in herpes simplex virus 41.. Like oriLyt of EBV, these flanking seq[r] ...
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cis- and trans-acting elements involved in reactivation of vaccinia virus early transcription.
... in DNA replication and transcription of intermediate ...viral DNA from the cores and makes it accessible to replication. DNA replica- tion marks the end of the early phase and the onset of tran- ...
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Attenuation of Herpes Simplex Virus Neurovirulence with Picornavirus cis-Acting Genetic Elements
... Southern blot. Following two rounds of plaque purification on Vero cells, the genetic structures of virus recombinants were assessed by Southern blot analysis. Viral DNA was isolated and prepared for Southern ...
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Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields
... the cis-acting elements used to facilitate the expression of the genes of interest and the selectable marker genes from these cassettes remains ...specific DNA sequences, that have been ...
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Promoter analysis by saturation mutagenesis
... the DNA sequence, generally directly upstream to the coding sequence, required for basal and/or regulated transcription of a ...for cis-acting elements and subsequently define the critical ...
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A cis-acting DNA signal for encapsidation of simian virus 40.
... Since the ability to yield CAT enzymatic activity depends not only upon gene transmission but also on gene expression, and since regulatory elements of SV40 were deleted from pSO-y-S mos[r] ...
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Bovine leukemia virus transcription is controlled by a virus-encoded trans-acting factor and by cis-acting response elements.
... Like the human T-cell leukemia viruses type I and type II, but unlike other RNA tumor viruses, BLV contains several open reading frames at the 3' end of its genome.. A subgenomic mRNA wh[r] ...
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Phylogenetic prediction of cis acting elements: a cre like sequence in Norovirus genome?
... ble genome regions for an in-depth experimental analysis allowing establishing the role of the identified secondary RNA structures in translation or replication. This approach has permitted to raise the hypothesis that ...
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Approaches for establishing the function of regulatory genetic variants involved in disease
... by cis-regulatory variants rather than genotype-independent allele-specific epigenetic ...to cis-eQTL mapping and can provide mechanistic evidence of regulatory variants acting in ...
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Genomewide Location Analysis of Candida albicans Upc2p, a Regulator of Sterol Metabolism and Azole Drug Resistance
... Bioinformatic analyses. For the analysis of overrepresented functional cate- gories in the grouping of Upc2 targets (Table 3), 37 of the 202 Upc2-bound targets (P ⬍ 0.05) were removed from consideration, since their ...
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Q beta RNA bacteriophage: mapping cis-acting elements within an RNA genome.
... Two separate classes of mutant QII phage genomes 35 different insertion mutants, each containing an insertion of 3 to 15 nucleotides within the replicase gene, and 41 deletion genomes, e[r] ...
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p33-Independent Activation of a Truncated p92 RNA-Dependent RNA Polymerase of Tomato Bushy Stunt Virus in Yeast Cell-Free Extract
... RII(ⴙ)-SL cis-acting element in ...critical cis-acting elements required for template recognition [RII( ⫹ )- SL] and replicase assembly (SL1-SL2-SL3) (44, 46), were all used efficiently ...
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