cis-Acting replication element
Identification of a cis-Acting Replication Element within the Poliovirus Coding Region
... The replication of poliovirus, a positive-stranded RNA virus, requires translation of the infecting genome followed by virus-encoded VPg and 3D polymerase-primed synthesis of a negative-stranded ...picornavirus ...
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Anti-HCV RNA aptamers targeting the genomic cis-acting replication element
... 18. Diviney, S.; Tuplin, A.; Struthers, M.; Armstrong, V.; Elliott, R.M.; Simmonds, P.; Evans, D.J. A hepatitis C virus cis-acting replication element forms a long-range RNA-RNA interaction ...
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EWSR1 Binds the Hepatitis C Virus cis-Acting Replication Element and Is Required for Efficient Viral Replication
... Viral Replication Todd ...the cis-acting replication element (CRE), is located within the NS5B coding ...viral replication, the function of the interaction, and its regulation ...
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Poliovirus cis-Acting Replication Element-Dependent VPg Uridylylation Lowers the Km of the Initiating Nucleoside Triphosphate for Viral RNA Replication
... A cis-acting replication element (CRE) within picornavirus RNAs serves as a template for the uridylylation of VPg, resulting in the synthesis of VPgpUpU OH ...RNA replication and that ...
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A cis-Acting Replication Element in the Sequence Encoding the NS5B RNA-Dependent RNA Polymerase Is Required for Hepatitis C Virus RNA Replication
... the replication of RNA ...RNA replication, implicating it as an essential cis-acting replication element ...RNA replication, and the upper helix appears to serve as an ...
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Sequence Requirements for Viral RNA Replication and VPg Uridylylation Directed by the Internal cis-Acting Replication Element (cre) of Human Rhinovirus Type 14
... the cis-acting signals required for replication of picornaviral RNAs were believed to be restricted to the 5 ⴕ and 3 ⴕ noncoding regions of the ...internal cis-acting replication ...
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Mutational Disruption of cis-Acting Replication Element 2C in Coxsackievirus B3 Leads to 5′-Terminal Genomic Deletions
... transcribed RNA produces infectious CVB3 particles that can be isolated and transferred to fresh cell cultures. Inoculation of CVB3 and CVB3-CKO RNA into mice gener- ates virus. Our observations that CVB3-CKO produced ...
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Identification and Characterization of a cis-Acting Replication Element (cre) Adjacent to the Internal Ribosome Entry Site of Foot-and-Mouth Disease Virus
... for replication, since functional replicons and viruses lacking the 5 ⴕ cre could be obtained if a wild-type cre was added to the genome following the 3D pol coding ...in replication and demonstrate that ...
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A Hepatitis C Virus cis-Acting Replication Element Forms a Long-Range RNA-RNA Interaction with Upstream RNA Sequences in NS5B
... for replication, with the appearance of a limited number of colonies due to the acquisition of one or more compensatory mutations that restore replicative ...
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A hepatitis C virus cis acting replication element forms a long range RNA RNA interaction with upstream RNA sequences in NS5B
... Many viruses are known to possess pseudoknots that contribute essential functions 585 during the replication cycle. In most viruses, pseudoknots located within coding regions are primarily involved in ...
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A cis-Acting Element That Directs Circular Adeno-Associated Virus Replication and Packaging
... These enzymes would unambiguously distinguish between dif- ferent replicative intermediates as well as input plasmid DNA. DpnI selectively cleaves methylated input plasmid but is inac- tive against templates that have ...
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A new cis-acting element for RNA replication within the 5' noncoding region of poliovirus type 1 RNA.
... Indeed, the stem-loop II structure (nt 124 to 162) has been shown to be involved in IRES function (22, 31) (Fig. 8). Fur- thermore, Angel del Maria et al. (4) have reported that two segments, nt 97 to 182 and nt 510 to ...
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The Red Clover Necrotic Mosaic Virus RNA2 trans-Activator Is Also a cis-Acting RNA2 Replication Element
... the cis-acting sequences that are required for RNA2 replication in cowpea protoplasts, using a series of deletion mutants in the presence of ...However, replication of RNA2 was not affected by ...
Fine Mapping of a cis-Acting Sequence Element in Yellow Fever Virus RNA That Is Required for RNA Replication and Cyclization
... Although the sequences responsible for cyclization have not been identified, thermodynamic studies have shown that cy- clization results from hydrogen bonding, that the circular form is stable at 37°C under physiological ...
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Stem-Loop III in the 5′ Untranslated Region Is a cis-Acting Element in Bovine Coronavirus Defective Interfering RNA Replication
... 3). In pSLIII-mut2L, 5 of the 8 bases in the left arm were FIG. 2. Integrity of stem III is required for DI RNA replication as determined by analysis of mutant 1 variants of pDrep1. The mfold-predicted structures ...
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Stem-Loop IV in the 5′ Untranslated Region Is a cis-Acting Element in Bovine Coronavirus Defective Interfering RNA Replication
... DISCUSSION In this study, we have shown by enzyme structure probing the existence of a predicted bulged stem-loop IV mapping at nt 186 through 215 and therefore lying mostly within the 210-nt 5 ⬘ UTR of BCoV DI RNA. ...
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Novel cis-Acting Element within the Capsid-Coding Region Enhances Flavivirus Viral-RNA Replication by Regulating Genome Cyclization
... several cis-acting elements have been iden- tified in both the 5= and ...the cis-acting RNA elements of ...SL6 element (27) shares homology with stem 1 of DCS-PK; however, these two ...
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An RNA Stem-Loop within the Bovine Coronavirus nsp1 Coding Region Is a cis-Acting Element in Defective Interfering RNA Replication
... RNA replication, a set of 3 ⬘-terminal in-frame deletions of this region in pDrep1 was made by overlap PCR mutagenesis, and T7 RNA polymerase- generated transcripts of each mutant were tested for replica- tion in ...
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The 3′ cis-Acting Genomic Replication Element of the Severe Acute Respiratory Syndrome Coronavirus Can Function in the Murine Coronavirus Genome
... As has been pointed out by others (31), intragroup compat- ibility between coronavirus RNA polymerases and cis-acting genomic sequences makes recombination between viral species more likely in the event of ...
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Efficient Replication of Adeno-Associated Virus Type 2 Vectors: a cis-Acting Element outside of the Terminal Repeats and a Minimal Size
... additional cis-acting feature of either dimer- length replicative-form DNA or vGET015 may affect the ac- cumulation of extended ...negative-acting cis element in vGET015 but not vsub, ...
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