cleavage site
Genetic Predisposition To Acquire a Polybasic Cleavage Site for Highly Pathogenic Avian Influenza Virus Hemagglutinin
... editing site of the ebolavirus GP gene dramatically reduced the RNA editing efficiency, whereas other mutations irrelevant to the destabilization of the stem-loop structure did not affect the editing ...HA ...
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A vitellogenin polyserine cleavage site: highly disordered conformation protected from proteolysis by phosphorylation
... exact cleavage sites in the AmVg polypeptide, both the 150 and 40kDa Vg fragments were subjected to N-terminal ...the cleavage site between the 40 and 150kDa fragments, both fragments were ...
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Mutagenesis of the yellow fever virus NS2B/3 cleavage site: determinants of cleavage site specificity and effects on polyprotein processing and viral replication.
... for cleavage at certain ...for cleavage site specificity (16), the relative importance of amino acid residues within the con- sensus cleavage site as determinants of cleavage ...
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Analysis of cleavage site mutations between the NC and PR Gag domains of Rous sarcoma virus.
... NC-PR cleavage site mutants like cs22. If proper processing at this site cannot occur, cleavage is redirected to the neighboring site three amino acids downstream in the PR domain, ...
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Identification of the murine coronavirus p28 cleavage site.
... efficient cleavage of ...on cleavage was assayed by using the rabbit reticulocyte coupled transcription-translation system as de- scribed ...on cleavage of p28. To quan- titate the inhibition of ...
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Insights into Avian Influenza Virus Pathogenicity: the Hemagglutinin Precursor HA0 of Subtype H16 Has an Alpha-Helix Structure in Its Cleavage Site with Inefficient HA1/HA2 Cleavage
... the cleavage site. (A to C) Electrostatic diagrams of cleavage sites show that the key R329 residue is far from the negatively charged cavity in H3HA0 (A), R329 covers the negatively charged cavity ...
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Hepatitis C virus-encoded NS2-3 protease: cleavage-site mutagenesis and requirements for bimolecular cleavage.
... 2/3 site, yielding cleavage products of 22 and 19 kDa, corresponding to truncated NS2 (2*) and NS3 (3*), ...2/3 cleavage site, including many noncon- servative changes, remarkably ...on ...
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Mutations affecting cleavage at the p10 capsid protease cleavage site block Rous sarcoma virus replication
... protease cleavage site within ...protease cleavage site is ...PR-mediated cleavage of peptides containing the wild type (PVVAM*PVVIKRR) and mutant p10-CA sites are also ...The ...
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Analysis of the cleavage site of the human immunodeficiency virus type 1 glycoprotein: requirement of precursor cleavage for glycoprotein incorporation.
... Endoproteolytic cleavage of the glycoprotein precursor to the mature SU and TM proteins is an essential step in the maturation of retroviral ...glycoproteins. Cleavage of the precursor polyprotein occurs at ...
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Newcastle Disease Virus Establishes Persistent Infection in Tumor Cells In Vitro: Contribution of the Cleavage Site of Fusion Protein and Second Sialic Acid Binding Site of Hemagglutinin-Neuraminidase
... ABSTRACT Newcastle disease virus (NDV) is an oncolytic virus being developed for the treatment of cancer. Following infection of a human ovarian cancer cell line (OVCAR3) with a recombinant low-pathogenic NDV, persistent ...
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Mutational analysis of the human immunodeficiency virus type 1 env gene product proteolytic cleavage site.
... The aim of this study has been to further analyze the cleavage site at the level of the individual amino acid and assess the importance of site 2 in protease activity by mutating the bas[r] ...
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Interplay between carbohydrate in the stalk and the length of the connecting peptide determines the cleavability of influenza virus hemagglutinin.
... Influence of stalk carbohydrate and the number of basic amino acids at the cleavage site on the biologic activities of the HA' Amino acid sequence at: Site-specific mutant Polykaryon Cle[r] ...
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Sequences within the Herpesvirus-Conservedpac1 and pac2 Motifs Are Required for Cleavage and Packaging of the Murine Cytomegalovirus Genome
... ectopic cleavage site insertion) and electroporated into NIH 3T3 cells as previously described ...HindIII site as the gpt/CX insertion ...same site would also grow normally in cultured ...
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Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii
... cleavage of the ⬃ 235-bp PCR product amplified from the hygromycin resistance genes when the DraIII site had not been ...enzyme site at the expected cleavage site for a Cas9/sgRNA ...
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Novel human endogenous sequences related to human immunodeficiency virus type 1.
... Amino acid comparison of the putative EHS-1 gene product with HIV-1 at the cleavage site reveals 50% identity across the domain shown in Fig.. Retrovirus envelope tryptic cleavage sites [r] ...
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Inhibition of Coxsackievirus associated dystrophin cleavage prevents cardiomyopathy
... 2A–mediated cleavage of dystrophin and subsequent disruption of the sar- colemma play a role in CV-mediated ...2A cleavage site of the dystrophin gene, which prevents dystrophin cleavage ...
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Minute Virus of Canines NP1 Protein Interacts with the Cellular Factor CPSF6 To Regulate Viral Alternative RNA Processing
... which cleavage and polyadenyl- ation events for (pA)p, we made mutations of these motifs either individually or in combination ...and cleavage was much more heterogenous, with a particular reduction in ...
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Proteomic database mining opens up avenues utilizing extracellular protein phosphorylation for novel therapeutic applications
... C-propeptide cleavage regions. One phos- phorylated site is located 16 residues downstream of the BMP1 cleavage site and 7 residues downstream of the meprin α and β cleavage site ...
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Active Residues and Viral Substrate Cleavage Sites of the Protease of the Birnavirus Infectious Pancreatic Necrosis Virus
... VP4 cleavage sites, located at the pVP2-VP4 and VP4-VP3 junctions, were identified by N-ter- minal sequence analysis of cleavage products produced in ...by site-directed ...tional cleavage ...
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Inhibition of Influenza Virus Infection in Human Airway Cell Cultures by an Antisense Peptide-Conjugated Morpholino Oligomer Targeting the Hemagglutinin-Activating Protease TMPRSS2
... HA cleavage by TMPRSS2 and not to nonspecific effects, viral growth of Mem- phis/96 (H1N1) in T-ex5-PPMO treated cells was monitored in the presence of exogenous ...multibasic cleavage site which can ...
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