Cloning and expressing recombinant eggshell proteins
Cloning and Expression of Recombinant Proteins
... Restriction-enzyme based cloning PCR DNA Sequencing Yeast genetics Eukaryotic protein expression systems Transfection of cell culture cells. Insect cell expression[r] ...
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Recombinant feline herpesviruses expressing feline leukemia virus envelope and gag proteins.
... The FeLV env glycoprotein expressed by recombinant FHV was processed and transported to the cell surface much as in FeLV infection, with the exception that proteolytic processing to yiel[r] ...
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Generation of Recombinant Rotaviruses Expressing Fluorescent Proteins by Using an Optimized Reverse Genetics System
... and recombinant rotaviruses carrying reporter genes will be great additions to the tool kit for studying the molecular virology of rotavirus and for developing future next-generation vaccines and expression ...
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Cloning, Phylogenetic Analysis and Expression of Recombinant LipL41, Loa22 and LipL21 Proteins from Leptospira interrogans
... develop recombinant outer membrane protein based diagnostic ...gene) proteins were purified by nickle-nitriloacetic acid (Ni-NTA) affinity ...Purified proteins were confirmed by sodium ...
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Comparative transcriptomic profile analysis of fed-batch cultures expressing different recombinant proteins in Escherichia coli
... with recombinant protein ...three proteins was to analyse the difference in the tran- scriptomic profile when two soluble proteins were expressed under different expression systems and also to see ...
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Synthesis of bluetongue virus (BTV) corelike particles by a recombinant baculovirus expressing the two major structural core proteins of BTV.
... frugiperda cells infected with the recombinant baculovirus synthesized two unique protein species which comigrated with VP3 and VP7 derived from purified BTV cores or virus particles (Fi[r] ...
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Recombinant Canine Distemper Virus Strain Snyder Hill Expressing Green or Red Fluorescent Proteins Causes Meningoencephalitis in the Ferret
... fluorescent proteins enhanced our ability to trace pathways of virus infection in the periphery and in the CNS and facilitated our understanding of how the virus spreads through meningeal cell layers at the sin- ...
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Compartmentalization of VP16 in Cells Infected with Recombinant Herpes Simplex Virus Expressing VP16-Green Fluorescent Protein Fusion Proteins
... of recombinant viruses ex- pressing the VP16 linked to the green fluorescent protein ...replication proteins and containing VP16 formed on the periphery of the replication compartments as infection pro- ...
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Induction of antigen specific immune responses in mice by recombinant baculovirus expressing premembrane and envelope proteins of West Nile virus
... whether recombinant baculovirus-immunized mice induce WNV-specific neutral- izing antibodies, serum samples were tested by ...that recombinant baculoviruses immunized mice developed humoral immune responses ...
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Construction and immune efficacy of recombinant pseudorabies virus expressing PrM E proteins of Japanese encephalitis virus genotype І
... the recombinant virus PRV TK − / gE − /PrM-E + against JEV ...the recombinant virus PRV TK − /gE − /PrM-E + survived without showing characteristic signs of ...
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Assembly of herpes simplex virus (HSV) intermediate capsids in insect cells infected with recombinant baculoviruses expressing HSV capsid proteins.
... structures that form the outer surface (icosahedral shell) of the capsid, (ii) the products of the UL26 and/or UL26.5 genes are required (as scaffolds) for assembly of 100-nm capsids, an[r] ...
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Coinfection with recombinant vaccinia viruses expressing poliovirus P1 and P3 proteins results in polyprotein processing and formation of empty capsid structures.
... Coinfection of HeLa cells with the Pl-expressing vaccinia virus and with a second recombinant vaccinia virus which expresses the poliovirus proteinase 3CD resulted in the correct process[r] ...
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Recombinant Rinderpest Vaccines Expressing Membrane-Anchored Proteins as Genetic Markers: Evidence of Exclusion of Marker Protein from the Virus Envelope
... marked recombinant rinderpest vaccines using either the influenza virus HA protein or ANC-GFP as a ...of recombinant negative-strand RNA viruses have been constructed as vectors for the highly stable ...
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Greater Protection against Virulent Infectious Bronchitis Virus Challenge Conferred by Recombinant Baculovirus Co-expressing S1 and N Proteins
... Gene cloning and the recombinant baculoviruses’ construction In order to increase expression and yield of heterologous secreted proteins in insect cells, the HBM signal peptide recognized by insect ...
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Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein
... of recombinant antigens representative of acute or chronic infection might further enhance their application for discrim- inating acute from chronic infection ...surface proteins have been reported as ...
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Cloning and Characterization of Multigenes Encoding the Immunodominant 30 Kilodalton Major Outer Membrane Proteins ofEhrlichia canis and Application of the Recombinant Protein for Serodiagnosis
... a recombinant 28-kDa protein (rP28), one of the outer membrane proteins of a gene (omp-1) family of Ehrlichia ...30-kDa proteins and the major surface proteins of the rickettsiae revealed that ...
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Biophysical characterization of recombinant proteins expressing the leucine zipper-like domain of the human immunodeficiency virus type 1 transmembrane protein gp41.
... tetramers. Recombinant proteins containing an alanine or a serine substitution at a critical isoleucine residue within the zipper region were also generated and similarly ...serine-substituted ...
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Neutralizing Antibodies Elicited by Immunization of Monkeys with DNA Plasmids and Recombinant Adenoviral Vectors Expressing Human Immunodeficiency Virus Type 1 Proteins
... Recombinant rAd5 viruses with deletions in E1 and E3 were generated by a modification of a previously published method (24). The HxB2/BaL and 89.6P Env rAd5 constructs expressed gp140 rather than the gp145 protein ...
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Immunodiagnosis of Ehrlichia canis Infection with Recombinant Proteins
... North Carolina State University, Raleigh, North Carolina 27606 3 Received 11 May 2000/Returned for modification 24 July 2000/Accepted 27 September 2000 Ehrlichia canis causes a potentially fatal rickettsial disease of ...
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Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata
... silk proteins potentially leads to the development of new functional biofiber ...silk proteins (Smsps), Smsp-1, Smsp-2, Smsp-3, and Smsp-4 from silk glands of ...
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