Cloning and protein expression
Gene cloning and recombinant protein expression of lipase from marine bacterium
... June 2014 Chairman : Adam Leow Thean Chor, PhD Faculty : Biotechnology and Biomolecular Sciences Nowadays salt tolerant enzymes have gained attention since they have advantages in food processing industries and ...
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Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
... fusion protein. To further improve protein solubility the polar amino acids lysine and glutamine can also be used in the ...of protein solubility prediction online software, the solubilities of HspX ...
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Cloning and Expression of Recombinant Proteins
... Restriction-enzyme based cloning PCR DNA Sequencing Yeast genetics Eukaryotic protein expression systems Transfection of cell culture cells. Insect cell expression[r] ...
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Cloning and expression of cold-inducible RNA binding protein in domestic yak (Bos grunniens)
... gene expression. Cold-inducible RNA binding protein (CIRP) is activated by hypothermia, hypoxia and UV radiation; moreover, several reports have noted that CIRP activation could also be found in response to ...
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Cloning and Expression of a Human T-Lymphotropic Virus Type 1 Protein with Reverse Transcriptase Activity
... the cloning of the predicted HTLV-1 RT by using a novel approach that allows the pro- duction and purification of a protein with RT ...RT expression clone was simple and ...modern expression ...
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Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis
... replication protein has phosphatase activity, ATP-ase activity and is able to unwind double strand DNA ...replication protein with GFP protein was used to monitor protein expression by ...
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Pirin-Like Protein from Pseudomonas stutzeri Zobell:Gene Cloning, Heterologous Expression, and Its Quercetinase Activity
... pirin-like protein gene to the reported Pseudomonas stutzeri Zobell draft ...pirin-like protein expression. The expressed protein was extracted by sonication for breaking the host cells which ...
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Cloning and Expression of a Helicobacter bilis Immunoreactive Protein
... DISCUSSION Sera from mice infected with H. bilis react with a number of proteins that could serve as antigens for serodiagnosis, if cloned and expressed in recombinant form. Membrane anti- gens are logically favored ...
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Cloning and expression of an equine herpesvirus 1 origin-binding protein.
... The product of EHV-1 gene 53, which will be referred to as the EHV-1 OBP, also bound specifically to a truncated HSV-1 Oris and recog- nized a consensus binding site similar to that reco[r] ...
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Cloning, expression and characterization of gE protein of Duck plague virus
... gE protein of duck plague virus is the important membrane glycoprotein, its protein characterization has not been ...pET32a/DPV-gE protein was highly expressed after induction by ...fusion ...
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Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein
... Early diagnosis of the disease is of great impor- tance in the prevention of the disease, and planning for effective treatments, in high risk people like im- munocompromised patients and pregnant women. One of the main ...
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Cloning, Expression, and Immunological Characterization of the P30 Protein of Mycoplasma pneumoniae
... P30 protein have mainly been fo- cused on understanding its role in cytadherence and gliding motility (9, 11, 16, ...fusion protein. The fusion protein was immunogenic, as it elicited a boostable ...
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Cloning and expression of a chitinbinding protein of "Pseudoalteromonas tunicata" CCUG 44952T
... La identificación, clonación, secuenciación y estudio de un gen de la bacteria halófila moderada Pseudoalteromonas tunicata , cepa CCUG 44952T, que codifique una nueva proteína [r] ...
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Cloning and Expression of Coxsakievirus B3 Viral Protein-1 in E. Coli
... Cloning and Expression of Coxsakievirus B3 Viral Protein-1 in E. Coli Nader Maghsoudi *1 , Akbar Khalilpour 2 , Mahdi Kamali 2 and Mahdi Zeinoddini 3 1 Neuroscience Research Center, Shahid Beheshti ...
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Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... To determine the immunogenicity of the baculovirus expressed VP4, rabbits were immunized with the VP4 lysate as described in Methods. The ability of rabbit antiserum to recognize VP4 in intact SA11 was investigated by ...
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Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.
... DNA sequence analysis of part of the human herpesvirus 6 (HHV-6) genome led to the identification of an open reading frame with amino acid sequence homology to the major capsid proteins [r] ...
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Cloning, expression, purification and characterization of a DsbA-like protein from Wolbachia pipientis
... METHODS Cloning Wolbachia pipientis -DsbA1 Wolbachia pipientis wMel strain was maintained in Drosophila melanogaster yw67c23 flies reared on standard corn flour-sugar-yeast medium at ...
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Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein
... CSUI_005805 protein was cloned into the bacterial expression vector pQE-31 to express the recombinant protein rCSUI_005805 in a size similar to the predicted molecular mass that was present in ...
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Helicobacter pylori recombinant UreG protein: cloning, expression, and assessment of its seroreactivity
... Akbar Khalilpour 1 , Sabariah Osman 1 , Muhammad Hafiznur Yunus 1 , Amutha Santhanam 1 , Nagarajan Vellasamy 2 and Rahmah Noordin 1* Abstract Background: Helicobacter pylori is a human pathogen and during the process of ...
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Human pancreatitis associated protein Messenger RNA cloning and expression in pancreatic diseases
... associated protein" (PAP). The selected clone encoded a secretory protein structurally related to rat ...The protein had the same size as rat PAP and showed 71% amino acid identity, the six ...
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