Cloning and Purification
Use of monoclonal antibodies to facilitate identification, cloning, and purification of Chlamydia trachomatis hsp10
... Vector and cloning strategy. The pQE-60 vector (QIAGEN, Inc., Chatsworth, Calif.) was chosen to append a tag of six histidines (His 6 ) to the C terminus of chsp10 for large-scale purification via nickel ...
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Cloning, Expression, and Purification of HIV-2 gp125
... The envelope of the human immunodeficiency virus (HIV) is the main target for neutralizing antibodies. We report the cloning, purification, and characterization of two recombinant forms of the envelope ...
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Cloning, Expression, Purification, and Crystallization of P aeruginosa ICMP
... DOI: 10.4236/ajmb.2018.84017 197 American Journal of Molecular Biology ter arcticus (IPPA), the permuted imelysin from Bacteroides ovatus (PIBO) [18], and the alginate-binding protein from Sphingomonas sp. A1 (Algp7) ...
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Molecular Cloning, Expression And Purification Studies With An ORF Of Mycobacterium Tuberculosis
... a cloning vector pGEM-T Easy and transformed in cloning host ...The purification protocol was established for purification from inclusion ...
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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
... Cloning of Pwo polymerase gene. Reactions were performed according to the manufacturer’s instructions. PCR product was cloned into pENTR/ SD/D-TOPO vector (Invitrogen, USA) and then Pwo polymerase gene was ...
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Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon
... Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon James H. Dyer 1, , Anthony Maina 1 , Iris D. Gomez 1 , Melissa Cadet 1 , Silke Oeljeklaus 2 , Anke C. Schiedel 3 ...
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Cloning, expression, purification and characterization of two diagnostically important proteins of Brucella abortus
... Key words: Brucellosis, Brucella abortus, cloning, expression, purification INTRODUCTION Brucella infection causes contagious abortions in cattle and “undulant fever’ in humans. The infection is wide spread ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... Cloning of ESAT-6 andCFP-10. Each PCR product was ligated into pTZ57R ⁄T cloning vector after purification using QIA quick PCR purification kit (MBI Fermentas, Lithuania). Consequently, ...
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New Tannase-Producing Lactobacillus Sp. Nrc10: Gene Cloning, Enzyme Purification, And Characterization
... The cloning of tannase gene (Tanlp1) was carried out and its complete sequence was ...The cloning and efficient expression of tannase gene (Tanlp1) were carried ...and purification of the tannase ...
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Cloning, expression and purification of the factor H binding protein and its interaction with factor H
... the cloning and ex- pression of recombinant proteins in ...sub cloning of 820 bp fHBP gene into pET 28a (+) expression vec- tor was carried out by enzymatic ...its purification will be very ...
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Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
... Gel Purification Kit (GeNet Bio, Korea) and ligated into the multiple cloning site (MCS) of the T7lac promoter expression plasmid pET-21b(+) with T4 DNA ligase (5 U/µL) (Thermo Fisher Scientific, ...the ...
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Cloning, expression, purification, crystallization and preliminary X ray diffraction of a lysine specific permease from Pseudomonas aeruginosa
... on homology modelling, a number of residues that impact on lysine transport by LysP from Salmonella typhimurium have been identified (Kaur et al., 2014). Isothermal titration calorimetry was used to establish that ...
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Cloning, expression, purification and in silico analysis of n terminal domain of pneumococcal surface protein a from all six clades
... 1 Molecular Immunology Laboratory, National Institute of Immunology, New Delhi, India 2 Department of Biotechnology, University of Kashmir, Srinagar, India ARTICLE INFO ABSTRACT Streptococcus pneumoniae (or pneumococci) ...
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Cloning, overexpression and purification of Escherichia coli tRNAIle and tRNAVal
... tRNA Ile i utvrđivanje broja i tipa posttranskripcijskih modifikacija, biti će potrebne analize pojedinačnih nukleozida tRNA metodom spektrometrije mase... Prilikom konstrukc[r] ...
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Cloning, purification, and biochemical characterization of the pneumococcal bacteriophage Cp-1 lysin.
... Inhibition of lysis by antibody against phage-associated lysin and requirement of choline residues in the cell wall for progeny phage release in Streptococcus pneumoniae. Nucleotide sequ[r] ...
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Cloning, purification and characterization of the ribosomal protein L11 from E coli
... 2.2. Purification of L11 under Native Conditions A single colony of E. coli BL21, transformed with pET-22b/L11, was grown in LB with Amp to 100 μg/ml at 37˚C to OD 550 = 0.6 and induced with IPTG to 1 mM for 2.5 ...
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Cloning, expression, purification and characterization of a DsbA-like protein from Wolbachia pipientis
... METHODS Cloning Wolbachia pipientis -DsbA1 Wolbachia pipientis wMel strain was maintained in Drosophila melanogaster yw67c23 flies reared on standard corn flour-sugar-yeast medium at ...
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Discovery, Cloning, Expression, Purification, and Characterization of Phosphoglycolate Phosphatase from Staphylococcus aureus
... used to measure the concentration of inorganic phosphate released by the enzyme, as described. above[r] ...
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Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii
... after cloning into an expression vector, the expressed and purified protein was used for evaluation of immunoreactivity of the synthetic protein, using human ...
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Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities
... From purification of 5 mg/mL of protein extract into a size exclusion chromatography, seven fractions were collected, numbered 16–22, and were selected for further ...
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