Cloning, Expression, Purification and Functional Characterisation
Molecular Cloning, Expression And Purification Studies With An ORF Of Mycobacterium Tuberculosis
... for expression and production of large scale protein and to develop its purification ...a cloning vector pGEM-T Easy and transformed in cloning host ...colonies. Expression vector ...
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Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon
... Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon James H. Dyer 1, , Anthony Maina 1 , Iris D. Gomez 1 , Melissa Cadet 1 , Silke Oeljeklaus 2 , Anke C. ...
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Cloning, expression, purification and characterization of two diagnostically important proteins of Brucella abortus
... Key words: Brucellosis, Brucella abortus, cloning, expression, purification INTRODUCTION Brucella infection causes contagious abortions in cattle and “undulant fever’ in humans. The infection is wide ...
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Cloning, expression and purification of the factor H binding protein and its interaction with factor H
... pET expression system has been developed for the cloning and ex- pression of recombinant proteins in ...sub cloning of 820 bp fHBP gene into pET 28a (+) expression vec- tor was carried out by ...
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Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
... Gel Purification Kit (GeNet Bio, Korea) and ligated into the multiple cloning site (MCS) of the T7lac promoter expression plasmid pET-21b(+) with T4 DNA ligase (5 U/µL) (Thermo Fisher Scientific, ...
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Cloning, Expression, and Purification of HIV-2 gp125
... the cloning, purification, and characterization of two recombinant forms of the envelope glyco- protein gp125 from a primary HIV-2 SBL-6669 ...and functional analyses, as well as vaccine ...
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Cloning, Expression, Purification, and Crystallization of P aeruginosa ICMP
... The insulin-cleaving membrane protease (ICMP) of P. aeruginosa plays a vi- tal role in the pathogenesis of the bacterium and is therefore characterized as an important bacterial virulence factor. In addition, ICMP also ...
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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
... Cloning of Pwo polymerase gene. Reactions were performed according to the manufacturer’s instructions. PCR product was cloned into pENTR/ SD/D-TOPO vector (Invitrogen, USA) and then Pwo polymerase gene was ...
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Purification, Cloning, and Expression of an Apyrase from the Bed Bug Cimex lectularius
... Expression of Cimex Apyrase in COS-7 Cells—COS-7 cells (ATCC CRL 1651) were cultivated in a humidified 37 °C incubator with 5% CO 2 in Dulbecco’s modified Eagle’s medium supplemented with 4 m M glu- tamine, 100 ...
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Cloning, expression, purification and characterization of a DsbA-like protein from Wolbachia pipientis
... METHODS Cloning Wolbachia pipientis -DsbA1 Wolbachia pipientis wMel strain was maintained in Drosophila melanogaster yw67c23 flies reared on standard corn flour-sugar-yeast medium at ...
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Discovery, Cloning, Expression, Purification, and Characterization of Phosphoglycolate Phosphatase from Staphylococcus aureus
... 52 x. Overexpression of SaPGPase in E.coli BLR(DE3) S. aureus PGPase (without a His•Tag) was overexpressed in E. coli BLR(DE3) at 37 °C. The cells were harvested 3 hours after induction with IPTG and underwent two rounds ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... Cloning of ESAT-6 andCFP-10. Each PCR product was ligated into pTZ57R ⁄T cloning vector after purification using QIA quick PCR purification kit (MBI Fermentas, Lithuania). Consequently, ...
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Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii
... after cloning into an expression vector, the expressed and purified protein was used for evaluation of immunoreactivity of the synthetic protein, using human ...
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Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities
... constitutive expression systems, ...inducible expression systems produce active clavMO fused to thioredoxin against both Gram-negative Klebsiella pneumoniae and Gram-positive Staphylococcus aureus ...
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Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis
... The total protein yield at the last purification step was approximately 71 mg of Rep 34 per liter of bacterial cul- ture. Upon SDS–PAGE under reducing conditions, the iso- lated protein migrated as a large ...
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Cloning and Expression of the algl Gene, Encoding the Azotobacter chroococcum Alginate Lyase: Purification and Characterization of the Enzyme
... (1). DNA probes were 32 P labelled with a DNA-labelling kit (-dCTP) (Pharma- cia) and [a- 32 P]dCTP (Amersham). Cloning of the algL gene. Genomic DNA from A. chroococuum was completely digested with EcoRI and ...
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CLONING, PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF SUGARCANE ENDOGENOUS AZOTOBACTER KLEBSIELLA VARIICOLA DX120E NIFK GENE
... prokaryotic expression protein by induction was detected by mass spectrometry, which had an isoelectric point of ...prokaryotic expression system, which laid the foundation for further studying the function ...
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Cloning, expression and purification of resistance gene analogue RGPM 301 from pearl millet in Escherichia coli
... Plants combat their pathogens with an array of defense responses. One of the key mechanisms involves products of resistance (R) genes which are responsible for recognition of effector molecules from pathogens and ...
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The cloning and functional characterisation of a family of human stargazin-like genes
... Chapter 4_______________________________ In vitro expression and tissue distribution ju s t represent an extension of the CA3 region I have labelled it CA4 in diagram s and figures for ease o f description. The ...
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Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.)
... purification, cloning, and func- tional characterization of a 6-FEH from wheat (Triticum aestivum ...heterologous expression in the yeast Pichia pastoris showed—as did the native enzyme from wheat—a very ...
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