Cloning, protein expression and purification
Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
... fusion protein. To further improve protein solubility the polar amino acids lysine and glutamine can also be used in the ...of protein solubility prediction online software, the solubilities of HspX ...
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Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis
... replication protein has phosphatase activity, ATP-ase activity and is able to unwind double strand DNA ...replication protein with GFP protein was used to monitor protein expression by ...
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Molecular Cloning, Expression And Purification Studies With An ORF Of Mycobacterium Tuberculosis
... for expression and production of large scale protein and to develop its purification ...a cloning vector pGEM-T Easy and transformed in cloning host ...colonies. Expression ...
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Cloning, expression, purification and characterization of two diagnostically important proteins of Brucella abortus
... recombinant protein expression were obtained from Qiagen, ...DNA purification kit, PCR product purification kit were also procured from Qiagen, ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... Protein expression of Recombinant ESAT-6 ...Germany) expression host with antibiotics (100 µg⁄mL ampicillin) and incubated at 37°C until an optical density of ...recombinant protein. The ...
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Cloning, optimization of induction conditions and purification of Mycobacterium tuberculosis Rv1733c protein expressed in Escherichia coli
... Comprehensive data mining and bioinformatic analyses have identified Rv1733c as the 14th most promising candidate among 189 putative vaccine candidates from the entire genome of M. tuberculo- sis (20). Our ...
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Gene cloning and recombinant protein expression of lipase from marine bacterium
... molecular cloning, expression and purification techniques had led to rapid discovery of new isolates with unique ...properties. Expression in heterologous host helps in overcomes the ...
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Cloning, expression, purification and characterization of a DsbA-like protein from Wolbachia pipientis
... METHODS Cloning Wolbachia pipientis -DsbA1 Wolbachia pipientis wMel strain was maintained in Drosophila melanogaster yw67c23 flies reared on standard corn flour-sugar-yeast medium at ...
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Cloning, Expression, and Purification of HIV-2 gp125
... the cloning, purification, and characterization of two recombinant forms of the envelope glyco- protein gp125 from a primary HIV-2 SBL-6669 ...
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Cloning, expression and purification of the factor H binding protein and its interaction with factor H
... pET expression system has been developed for the cloning and ex- pression of recombinant proteins in ...sub cloning of 820 bp fHBP gene into pET 28a (+) expression vec- tor was carried out by ...
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Cloning, Expression, Purification, and Crystallization of P aeruginosa ICMP
... the protein remain ...crystallizable protein species is the prerequisite for structural studies by crystallography, we re- ported the successful expression, purification, and crystallization ...
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CLONING, PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF SUGARCANE ENDOGENOUS AZOTOBACTER KLEBSIELLA VARIICOLA DX120E NIFK GENE
... prokaryotic expression protein by induction was detected by mass spectrometry, which had an isoelectric point of ...nifK protein was successfully expressed in a prokaryotic expression system, ...
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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
... target protein contained 16 additional amino acid residues at the C-terminus, including six histidine residues for purification of the recombinant protein by Ni-NTA ...
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Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon
... sion are suitable because they are able to transport arabinose, but they can not metabolize it [19]. The His-tagged AACT was purified using the Ni-NTA affinity chromatography and a step-wise concentra- tion gradient of ...
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Purification, Cloning, and Expression of an Apyrase from the Bed Bug Cimex lectularius
... acidic protein with a pI of ...a protein of 364 amino acid residues, the first 35 of which constituted the signal ...the protein was predicted to have a molecular mass of ...
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Cloning, expression, purification and in silico analysis of n terminal domain of pneumococcal surface protein a from all six clades
... surface protein A (PspA) is one of the better defined pneumococcal virulence ...repot cloning, expression and purification of all six clades of PspA (extracellular domain ...
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Discovery, Cloning, Expression, Purification, and Characterization of Phosphoglycolate Phosphatase from Staphylococcus aureus
... 20 utilized to lower the imidazole concentration slowly to avoid protein denaturation and precipitation. In size exclusion chromatography, also known as gel filtration chromatography, a column filled with porous ...
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Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii
... Multi-epitopic protein based identification approach, using bioinformatics tools expected to be easier to use for standardization and diagnosis of the infection with more sensitivity and ...after cloning ...
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<p>In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment</p>
... recombinant protein vac- cines, as opposed to CRC for clinical function, extensive testing is ...Recombinant protein vaccines must be harmless, stable in various conditions, have high immuno- genicity, and ...
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Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities
... carrier protein and the construction was tested using two promoters, P AOX1 and P GAP , based on either induced or constitutive expression systems, ...inducible expression systems produce active ...
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