Cloning, protein expression, purification and crystallization
Cloning, Expression, Purification, and Crystallization of P aeruginosa ICMP
... DOI: 10.4236/ajmb.2018.84017 197 American Journal of Molecular Biology ter arcticus (IPPA), the permuted imelysin from Bacteroides ovatus (PIBO) [18], and the alginate-binding protein from Sphingomonas sp. A1 ...
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Cloning, expression, purification, crystallization and preliminary X ray diffraction of a lysine specific permease from Pseudomonas aeruginosa
... the cloning, recombinant expression, purification, crystallization and preliminary diffraction from crystals of LysP from the human pathogen ...
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Cloning, Purification, Crystallization and Preliminary X Ray Diffraction Studies of Periplasmic Glucose Binding Protein of Pseudomonas putida CSV86
... (2015) Cloning, Purification, Crystallization and Preliminary X-Ray Diffraction Studies of Periplasmic Glucose Binding Protein of Pseudomonas putida ...
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Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
... fusion protein. To further improve protein solubility the polar amino acids lysine and glutamine can also be used in the ...of protein solubility prediction online software, the solubilities of HspX ...
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Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis
... replication protein has phosphatase activity, ATP-ase activity and is able to unwind double strand DNA ...replication protein with GFP protein was used to monitor protein expression by ...
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Molecular Cloning, Expression And Purification Studies With An ORF Of Mycobacterium Tuberculosis
... for expression and production of large scale protein and to develop its purification ...a cloning vector pGEM-T Easy and transformed in cloning host ...colonies. Expression ...
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Cloning, expression, purification and characterization of two diagnostically important proteins of Brucella abortus
... recombinant protein expression were obtained from Qiagen, ...DNA purification kit, PCR product purification kit were also procured from Qiagen, ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... Protein expression of Recombinant ESAT-6 ...Germany) expression host with antibiotics (100 µg⁄mL ampicillin) and incubated at 37°C until an optical density of ...recombinant protein. The ...
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Cloning, expression, purification and characterization of a DsbA-like protein from Wolbachia pipientis
... METHODS Cloning Wolbachia pipientis -DsbA1 Wolbachia pipientis wMel strain was maintained in Drosophila melanogaster yw67c23 flies reared on standard corn flour-sugar-yeast medium at ...
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Protein Expression and Purification
... its expression level and protein assembly in ...plant expression vector under the control of CaMV 35S promoter and was introduced into Peperomia pellucida by biolistic transformation ...LTB ...
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Protein Expression and Purification
... multidimensional protein purification strategy for the parallel production of the catalytic G-do- mains of KRas, Rac1 and RalB GTPases in the active ...in protein production while generating products that ...
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Cloning, Expression, and Purification of HIV-2 gp125
... the cloning, purification, and characterization of two recombinant forms of the envelope glyco- protein gp125 from a primary HIV-2 SBL-6669 ...
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Cloning, expression and purification of the factor H binding protein and its interaction with factor H
... pET expression system has been developed for the cloning and ex- pression of recombinant proteins in ...sub cloning of 820 bp fHBP gene into pET 28a (+) expression vec- tor was carried out by ...
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CLONING, PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF SUGARCANE ENDOGENOUS AZOTOBACTER KLEBSIELLA VARIICOLA DX120E NIFK GENE
... prokaryotic expression protein by induction was detected by mass spectrometry, which had an isoelectric point of ...nifK protein was successfully expressed in a prokaryotic expression system, ...
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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
... target protein contained 16 additional amino acid residues at the C-terminus, including six histidine residues for purification of the recombinant protein by Ni-NTA ...
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Cloning and Expression of a Helicobacter bilis Immunoreactive Protein
... DISCUSSION Sera from mice infected with H. bilis react with a number of proteins that could serve as antigens for serodiagnosis, if cloned and expressed in recombinant form. Membrane anti- gens are logically favored ...
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Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon
... Figure 3. Alignment of 3’-RACE PCR product with A. thaliana thiolase (NM_203172). The putative sunflower AACT shares 70% sequence identity with the Arabidopsis sequence. Reverse complements of the underlined bold ...
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Purification, Cloning, and Expression of an Apyrase from the Bed Bug Cimex lectularius
... acidic protein with a pI of ...a protein of 364 amino acid residues, the first 35 of which constituted the signal ...the protein was predicted to have a molecular mass of ...
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Cloning, purification and characterization of the ribosomal protein L11 from E coli
... the protein [17]. The protein L11 has a high intrin- sic propensity to aggregate and extensively uses disor- dered regions to mediate and modulate interactions with other ...
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Expression, Purification and Crystallization of Thermostable Mutant of Cutinase Est1 from Thermobifida alba
... binant protein was purified by a two-step protocol involving immobilized metal affinity chromatography and cation-exchange chromatography, yielding 120 mg of protein per liter of bacterial ...
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