Clostridium autoethanogenum
Metabolic engineering of Clostridium autoethanogenum for selective alcohol production
... Gas fermentation using acetogenic bacteria such as Clostridium autoethanogenum o ff ers an attractive route for production of fuel ethanol from industrial waste gases. Acetate reduction to acetaldehyde and ...
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Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium
... Results: We performed next generation sequencing using Illumina MiSeq technology on the DSM10061 strain of Clostridium autoethanogenum and observed 243 single nucleotide discrepancies when compared to the ...
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Insights into CO2 Fixation Pathway of Clostridium autoethanogenum by Targeted Mutagenesis
... as Clostridium autoethanogenum are central to this bioprocess, but the molecular and genetic characterization of this microorganism is currently ...C. autoethanogenum, we identified the most ...
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Redox dependent metabolic shift in Clostridium autoethanogenum by extracellular electron supply
... Background: Microbial electrosynthesis is a novel approach that aims at shifting the cellular metabolism towards electron-dense target products by extracellular electron supply. Many organisms including several ...
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Clostridium autoethanogenum Fermentation Using Sugar and Gaseous Carbon Substrates.
... C. autoethanogenum synthesizes growth-associated ethanol to maintain pH while at the same time making acetate to provide ATP to meet cellular energy ...C. autoethanogenum cell will theoretically want to ...
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H2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum
... C. autoethanogenum proteins was performed using the bilayer reaction method with the wheat germ extract WEPRO8240H (Cell-Free Sci- ences) as described previously [56, ...C. autoethanogenum proteins was ...
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Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia
... In all Clostridium species that harbor CRISPR arrays, cas genes were identified. C. cellulolyticum and C. thermo- cellum had two and four different cas operons, respectively (Additional files 10 and 11). These cas ...
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Quantitative isotope dilution high resolution mass apectrometry analysis of multiple intracellular metabolites in Clostridium autoethanogenum with uniformly 13C labeled standards derived from Spirulina
... Optimization of the LC-HRMS Method for the Simultaneous Quanti fi cation of C. autoethanogenum Metabolites. The optimized ZIC-pHILIC LC-HRMS method provided the quantitative analysis of a standard mixture of 74 ...
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Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
... C. autoethanogenum, the CRISPR/Cas9 system requires the controlled expres- sion of cas9 and the constitutive expression of sgRNA in the presence of DNA editing ...for Clostridium beijerinckii is unclear ...
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Production of Bioethanol from Synthesis Gas Using Clostridium ljungdahlii as a Microbial Catalyst.
... described, Clostridium autoethanogenum, another acetogen able to produce ethanol from CO was isolated from rabbit feces (Abrini et ...C. autoethanogenum are ...C. autoethanogenum culture ...
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Pathogenicity Locus, Core Genome, and Accessory Gene Contributions to Clostridium difficile Virulence
... ABSTRACT Clostridium difficile is a spore-forming anaerobic bacterium that causes colitis in patients with disrupted colonic microbiota. While some individuals are asymptomatic C. difficile carriers, symptomatic ...
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Microbiome Data Distinguish Patients with Clostridium difficile Infection and Non C difficile Associated Diarrhea from Healthy Controls
... ABSTRACT Antibiotic usage is the most commonly cited risk factor for hospital-acquired Clostridium difficile infections (CDI). The increased risk is due to disruption of the indigenous microbiome and a subsequent ...
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The Complete Genome Sequence of Clostridium aceticum: a Missing Link between Rnf and Cytochrome Containing Autotrophic Acetogens
... Growth on CO (17) is possible by employing an additional CO FIG 1 TBLASTx comparison of the Wood-Ljungdahl and CO-dehydrogenase/acetyl-CoA synthetase gene clusters of C. aceticum with all other autotrophic acetogens ...
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Electron capture gas chromatography study of the acid and alcohol products of Clostridium septicum and Clostridium chauvoei
... The metabolic products produced by several strains of Clostridium septicum obtained from patients and animals, along with strains of Clostridium chauvoei, were studied in chopped meat gl[r] ...
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A helicase containing module defines a family of pCD630 like plasmids in Clostridium difficile
... A helicase containing module defines a family of pCD630 like plasmids in Clostridium difficile Accepted Manuscript A helicase containing module defines a family of pCD630 like plasmids in Clostridium[.] ...
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Switchgrass (Panicum virgatum) Fermentation by Clostridium thermocellum and Clostridium beijerinckii Sequential Culture: Effect of Feedstock Particle Size on Gas Production
... Fermentation of cellulosic biomass can be done in a single step with cellulolytic, solventogenic bacteria, such as Clostridium thermocellum. However, the suite of products is limited in consoli- dated ...
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Isolation and Identification of Polyhydroxyalkanoates from two Strains of Clostridium Bifermentans Isolated from the Soil Near the Gas Station in Basrah City
... Amplifying of the 16S rDNA Gene by (PCR) Technique: By using a universal primer, results have obtained the required band of 16S rDNA for the isolate along with electrophoresed ladder in the region of 1500bp, as in the ...
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Clostridium perfringens Epsilon Toxin Causes Selective Death of Mature Oligodendrocytes and Central Nervous System Demyelination
... ABSTRACT Clostridium perfringens epsilon toxin ( -toxin) is responsible for a devastating multifocal central nervous system (CNS) white matter disease in ruminant ...
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High Throughput Analysis of Gene Essentiality and Sporulation in Clostridium difficile
... ABSTRACT Clostridium difficile is the most common cause of antibiotic-associated intestinal infections and a significant cause of morbidity and mortality. Infection with C. difficile requires disruption of the ...
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16S rRNA Gene Sequencing, Multilocus Sequence Analysis, and Mass Spectrometry Identification of the Proposed New Species “Clostridium neonatale”
... among Clostridium neonatale, Clostridium butyricum, and Clostridium beijerinckii strains used in this ...of Clostridium butyricum strain 5521 (GenBank accession ...from Clostridium ...
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