Clostridium difficile toxin B
Recombinant Single-Chain Variable Fragment Antibodies Directed against Clostridium difficile Toxin B Produced by Use of an Optimized Phage Display System
... against Clostridium difficile toxin ...mouse B cell hybridoma line 5A8, which generates a monoclonal antibody against the ...obtain toxin B-binding scFv sequences derived from ...
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Evaluation of a New Commercial TaqMan PCR Assay for Direct Detection of the Clostridium difficile Toxin B Gene in Clinical Stool Specimens
... Wampole Clostridium difficile toxin B test (TOX-B test; TechLab, Blacksburg, VA), a cell culture cytotoxicity neutralization assay (CCCNA), and to anaerobic toxigenic bacterial culture, ...
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Clostridium difficile toxin B is more potent than toxin A in damaging human colonic epithelium in vitro
... C. difficile toxins A and B on human colonic mucosa in Ussing ...of toxin A and 0.2-29 nM of toxin B for 5 h caused dose-dependent epithelial ...of toxin A and by 76, 55, and ...
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Monoclonal antibodies specific for Clostridium difficile toxin B and their use in immunoassays
... difficile culture filtrate and Mono Q-purified fractions were incubated with these latex beads to bind the cytotoxin and then centrifuged to remove the beads, a drastic decrease of the t[r] ...
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Analysis of proteomes released from in vitro cultured eight Clostridium difficile PCR ribotypes revealed specific expression in PCR ribotypes 027 and 176 confirming their genetic relatedness and clinical importance at the proteomic level
... CDI: Clostridium difficile infection; DIGE: differ- ence gel electrophoresis; TcdA: Clostridium difficile toxin A; TcdB: Clostridium difficile toxin B; MS: ...
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Clostridium difficile Binary Toxin CDT Induces Clustering of the Lipolysis Stimulated Lipoprotein Receptor into Lipid Rafts
... We recently identified the lipolysis-stimulated lipoprotein recep- tor (LSR) as the host cell receptor for CDT and, moreover, showed FIG 4 LSR colocalizes with lipid raft marker proteins upon binding of CDTb. (A) H1-HeLa ...
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Performance of Clostridium difficile Toxin Enzyme Immunoassay and Nucleic Acid Amplification Tests Stratified by Patient Disease Severity
... C. difficile (Meridian Bioscience, Cincinnati, OH) assay, a NAAT that detects the presence of ...C. difficile through amplification of the 5= end of the toxin A ...C. difficile toxin ...
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Correlation of Disease Severity with Fecal Toxin Levels in Patients with Clostridium difficile Associated Diarrhea and Distribution of PCR Ribotypes and Toxin Yields In Vitro of Corresponding Isolates
... C. difficile were boiled in 10% Chelex, and PCR amplification of DNA interspaced between the genes encoding 16S and 23S rRNA was performed using Ready-to-Go PCR tubes (Amersham Biosciences) with primer pairs and ...
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Characteristics, Treatment, and Outcomes Associated with Clostridium difficile Associated Diarrhea in a Veterans Affairs Medical Center
... binary toxin has been present in the epidemic strain though its exact role is not well defined [8] [9] [12] ...binary toxin gene and more severe cases of C. difficile associated diarrhea ...of ...
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Molecular Analysis of the Pathogenicity Locus and Polymorphism in the Putative Negative Regulator of Toxin Production (TcdC) among Clostridium difficile Clinical Isolates
... of Clostridium difficile contains toxin A and B genes and three accessory genes, including tcdD and tcdC, which are supposed to code for the positive and negative regulators of toxin ...
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International Typing Study of Toxin A Negative, Toxin B Positive Clostridium difficile Variants
... of Clostridium difficile typically produce two high-molecular-weight toxins that share a considerable degree of homology and function but vary in respect to target cell ...specificity. Toxin A, the ...
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Pomegranate extract specifically inhibits <em>Clostridium difficile</em> growth and toxin production without disturbing the beneficial bacteria in vitro
... According to European Committee on Antimicrobial Sus- ceptibility, the MICs of pomegranate juice against different bacteria were determined in 96-well microtiter plate with round bottom. Different concentrations of ...
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The epidemiology of Clostridium difficile infection in Australia
... C. difficile colonization to active CDI is unknown; however, epidemiological studies have identified risk factors associated with progression to ...C. difficile colonization and disease because colonization ...
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Microbiome Data Distinguish Patients with Clostridium difficile Infection and Non C difficile Associated Diarrhea from Healthy Controls
... C. difficile using the ...C. difficile antigen glutamate dehydrogenase (GDH) and the C. difficile toxin proteins A and ...and toxin proteins, the sample was classified as a case or as a ...
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Prevalence and Genetic Characterization of Toxin A Variant Strains of Clostridium difficile among Adults and Children with Diarrhea in France
... A-negative, toxin B-positive strains) of Clostridium difficile have been reported to be responsible for diarrhea or pseudomembranous colitis in ...the toxin A gene (tcdA) and are ...
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Bacteriophage-Mediated Toxin Gene Regulation in Clostridium difficile
... Clostridium difficile has been identified as the most important single identifiable cause of nosocomial antibiotic-associated diarrhea and ...C. difficile produce two large protein toxins, ...
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Epidemiology of Clostridium difficile infection in hospitalized adults and the first isolation of C difficile PCR ribotype 027 in central China
... C. difficile associated and non-CDI as- sociated diarrhea ...formed Toxin A/B EIA test), currently more recom- mended methods for rapid diagnosis of CDI [2], should be ...C. difficile is high ...
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New Types of Toxin A Negative, Toxin B Positive Strains among Clostridium difficile Isolates from Asia
... C. difficile strains has already been applied to two large European strain collections from the Universite´ Catholique de Louvain in Brussels, Belgium (23), and the Anaerobe Reference Unit, PHLS, in Cardiff, Wales ...
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Cwp19 Is a Novel Lytic Transglycosylase Involved in Stationary Phase Autolysis Resulting in Toxin Release in Clostridium difficile
... analyzed by matrix-assisted laser desorption ionization–time of flight mass spectrom- etry (MALDI-TOF MS) and by MALDI-TOF/TOF tandem MS (MS/MS), and the resulting data were used to predict consistent structures (Table ...
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Comparison of Simplexa Universal Direct PCR with Cytotoxicity Assay for Diagnosis of Clostridium difficile Infection: Performance, Cost, and Correlation with Disease
... All study samples were stored at 4°C and tested by cytotoxicity assay within 24 h of receipt. Stool samples (0.5 ml) were added to 0.5 ml of phosphate-buffered saline with antibiotics (vancomycin, gentamicin, and ...
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