clustered regularly interspaced short palindromic repeat
Clustered Regularly Interspaced Short Palindromic Repeat Dependent, Biofilm Specific Death of Pseudomonas aeruginosa Mediated by Increased Expression of Phage Related Genes
... The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (CRISPR/Cas) system is an adaptive immune system present in many archaea and ...
13
Use of Clustered Regularly Interspaced Short Palindromic Repeat Sequence Polymorphisms for Specific Detection of Enterohemorrhagic Escherichia coli Strains of Serotypes O26:H11, O45:H2, O103:H2, O111:H8, O121:H19, O145:H28, and O157:H7 by Real Time PCR
... the clustered regularly interspaced short palindromic repeat (CRISPR) regions of enterohe- morrhagic Escherichia coli (EHEC) to design simplex real-time PCR assays for each of ...
6
New Clustered Regularly Interspaced Short Palindromic Repeat Locus Spacer Pair Typing Method Based on the Newly Incorporated Spacer for Salmonella enterica
... A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella ...
8
Disruption of human papillomavirus 16 <em>E6</em> gene by clustered regularly interspaced short palindromic repeat/Cas system in human cervical cancer cells
... Abstract: High-risk human papillomavirus (HPV), especially HPV16, is considered a main causative agent of cervical cancer. Upon HPV infection, the viral oncoprotein E6 disrupts the host tumor-suppressor protein p53, thus ...
8
Group B Streptococcus Vaginal Carriage in Pregnant Women as Deciphered by Clustered Regularly Interspaced Short Palindromic Repeat Analysis
... ABSTRACT We evaluated the diversity of group B Streptococcus (GBS) vaginal car- riage populations in pregnant women. For this purpose, we studied each isolate present in a primary culture of a vaginal swab using a new ...
9
Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus
... the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system for the direct cleavage of Bombyx mori nucleopolyhedrovirus (BmNPV) genome DNA ...
13
Abundant and Diverse Clustered Regularly Interspaced Short Palindromic Repeat Spacers in Clostridium difficile Strains and Prophages Target Multiple Phage Types within This Pathogen
... the clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) ...acquired short nucleotide sequences (spacers) to target homologous sequences ...
10
The evaluation and application of multilocus variable number tandem repeat analysis (MLVA) for the molecular epidemiological study of Salmonella enterica subsp. enterica serovar Enteritidis infection
... Molecular typing methods for discriminating dif- ferent bacterial isolates of the same species are essen- tial epidemiological tools for detecting the outbreak of foodborne diseases [4]. An approprite molecular typing ...
9
Brain tumor modeling using the CRISPR/Cas9 system: state of the art and view to the future
... CRISPR, clustered regularly interspaced short palindromic repeat; Cas9, CRISPR-associated protein 9; gRNA, guide RNA; sgRNA, single guide RNA; crRNA, CRISPR RNA; tracrRNA, ...
11
Global Study of IS6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection
... tuberculosis complex strains, the Zaragoza strain contains a copy of IS6110 in the DR region, which is the clustered regularly interspaced short palindromic repeat CRISPR locus of M.. bo[r] ...
7
Multiple Applications of a Transient CRISPR-Cas9 Coupled with Electroporation (TRACE) System in the Cryptococcus neoformans Species Complex
... (clustered regularly interspaced short palindromic repeat)-Cas9 coupled with Electroporation (TRACE) system for targeted genetic manipulations in the ...
16
Foamy Virus Vector Carries a Strong Insulator in Its Long Terminal Repeat Which Reduces Its Genotoxic Potential
... (clustered regularly interspaced short palindromic repeat)/Cas9-based assay was devised that allowed integration of the proviral se- quences of GV, LV, and FV, all carrying an ...
25
Deletion of CD163 Exon 7 Confers Resistance to Highly Pathogenic Porcine Reproductive and Respiratory Viruses on Pigs
... In short, the pigs were scored 0 for normal, 1 for mild inappetence or decreased demeanor, 2 for sneezing/coughing, diarrhea, or rubefaction, 4 for severe respiratory distress, lameness, blue ears, or stopping ...
13
What is the available evidence for the range of applications of genome-editing as a new tool for plant trait modification and the potential occurrence of associated off-target effects: a systematic map
... CRISPR/Cas Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein, TALENs Transcription Activator-Like Effector Nucleases, ZFN Zinc- Finger Nuclease, O[r] ...
33
Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production
... CRISPR: clustered regularly interspaced short palindromic repeats; TALEs: transcription activator-like effectors; ZFN: zinc-finger nucleases; LEA: lipid- extracted algal; RBS: ribosome-[r] ...
21
Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
... CRISPR/Cas9 has been adapted for gene knockout (KO) in eukaryotic cells. In this context, it was shown that the crRNA and tracrRNA can be fused together via a short linker sequence to form a single guide RNA ...
11
Approaches for establishing the function of regulatory genetic variants involved in disease
... The FANTOM5 project (for ‘functional annotation of the mammalian genome 5’) has recently published work complementing and extending ENCODE by using cap ana- lysis of gene expression (CAGE) and single-molecule se- ...
15
Holo Seq: single cell sequencing of holo transcriptome
... generate short cDNAs (200–300 bp) before library con- struction, Not I digestion only disrupts limited reads and causes no significant bias (Additional file 2: Table S1, S2; Additional file 1: Figure S3 a, ...
22
The zebrafish anatomy and stage ontologies: representing the anatomy and development of Danio rerio
... BSPO: Spatial Ontology; CARO: Common Anatomy Reference Ontology; CL: Cell Ontology; CRISPR: Clustered Regularly Interspaced Short Palindromic Repeats; EMAP: Mouse Gross Anatomy and Devel[r] ...
11
LULL(ed) into complacency: a perspective on licenses and stem cell translational science
... The cost of free, or the law of unintended consequences Another frequent problem is the unintended conse- quence of actions taken by government and universities in reaction to some of these issues. Universities have ...
9