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Co-IP

Hepatoma-derived growth factor/nucleolin axis as a novel oncogenic pathway in liver carcinogenesis

Hepatoma-derived growth factor/nucleolin axis as a novel oncogenic pathway in liver carcinogenesis

... analysis, co-IP and GST pull down assays to demonstrate HDGF directly binds to surface NCL and regulates the NCL membrane translocalization, NCL gene expression, NCL/PI3K/Akt pathway and oncogenic ...

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FoxG1 and TLE2 act cooperatively to regulate ventral telencephalon formation

FoxG1 and TLE2 act cooperatively to regulate ventral telencephalon formation

... in co-IP assays (except for a lower amount of immunoprecipitated FoxG1 with ...having co-factors and protein modifications present and, under these conditions, which better resemble those in vivo, we ...

10

Histone H3 Interacts and Colocalizes with the Nuclear Shuttle Protein and the Movement Protein of a Geminivirus

Histone H3 Interacts and Colocalizes with the Nuclear Shuttle Protein and the Movement Protein of a Geminivirus

... vivo co-IP experiments confirmed the interactions among NSP, MP, and histone H3, and in vivo co-IP experiments performed with Myc-tagged his- tone H3 allowed for the recovery of a complex ...

12

Rhesus Macaque Rhadinovirus Encodes a Viral Interferon Regulatory Factor To Disrupt Promyelocytic Leukemia Nuclear Bodies and Antagonize Type I Interferon Signaling

Rhesus Macaque Rhadinovirus Encodes a Viral Interferon Regulatory Factor To Disrupt Promyelocytic Leukemia Nuclear Bodies and Antagonize Type I Interferon Signaling

... To further investigate the interaction between R12-FLAG protein and PML-NBs, we analyzed the IFA results by z-stack analysis and confocal microscopy to better visualize the potential nuclear localization and interaction. ...

21

Podocin, a raft associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin

Podocin, a raft associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin

... by Co-IPs with cross-linked antibodies against podocin, nephrin, CD2AP, or lamin (negative control) from glomerular ...shows Co-IPs of CD2AP and nephrin with anti- podocin antibody or preimmune serum (pre) ...

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Evasion of Antiviral Immunity through Sequestering of TBK1/IKKε/IRF3 into Viral Inclusion Bodies

Evasion of Antiviral Immunity through Sequestering of TBK1/IKKε/IRF3 into Viral Inclusion Bodies

... We found that NSs could be coimmunoprecipitated co-IP with tank-binding kinase 1 TBK1, a key kinase in the activation of IRF3/7 and IFN-␤ signaling, and that the interaction was specific[r] ...

10

Protein Interactions on Telomeric Retrotransposons in Drosophila

Protein Interactions on Telomeric Retrotransposons in Drosophila

... Telomere length in Drosophila is maintained by targeted transposition of three non-LTR retrotransposons: HeT-A, TART and TAHRE (HTT), but understanding the regulation of this process is hindered by our poor knowledge of ...

7

Overexpressed HDAC8 in cervical cancer cells shows functional redundancy of tubulin deacetylation with HDAC6

Overexpressed HDAC8 in cervical cancer cells shows functional redundancy of tubulin deacetylation with HDAC6

... Alpha tubulin is a non-histone substrate of HDAC8 Identification of protein-protein interactions by mass spectroscopy followed by immunoprecipitation and co- IP experiments is a well-established and very ...

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Overexpression of N-terminal kinase like gene promotes tumorigenicity of hepatocellular carcinoma by regulating cell cycle progression and cell motility

Overexpression of N-terminal kinase like gene promotes tumorigenicity of hepatocellular carcinoma by regulating cell cycle progression and cell motility

... Using co-IP and mass spectrometry approach, we identified the large GTPase dynamin2 as an interacting protein of NTKL, which might be responsible for the phenotype alterations caused by NTKL overexpression, ...

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Annexin A2 antibodies but not inhibitors of the annexin A2 heterotetramer impair productive HIV 1 infection of macrophages in vitro

Annexin A2 antibodies but not inhibitors of the annexin A2 heterotetramer impair productive HIV 1 infection of macrophages in vitro

... and co-immunoprecipitation (co-IP) assays, we investigated whether A2 directly interacts with the primary HIV-1 envelope protein ...to co-IP with A2 (data not ...traditional ...

5

HVint: a strategy for identifying novel protein protein interactions in Herpes Simplex Virus Type 1

HVint: a strategy for identifying novel protein protein interactions in Herpes Simplex Virus Type 1

... 6 Co-Immunoprecipitation (Co-IP), in vitro binding assays, and protein cross-linking, which can be then analyzed by MS. PPIs can also be derived from Nuclear Magnetic Resonance (NMR), X- ray ...

58

Programmable Buttons User Guide Supplement to IP 212k, IP 230, IP 560, and BB24 Devices

Programmable Buttons User Guide Supplement to IP 212k, IP 230, IP 560, and BB24 Devices

... Step 6 Verify that the information is correct for the Site, Switch, and other fields, and then in the Assign To drop-down menu, click on the MAC address of the IP phone. (The name of the user may appear next to ...

16

Controversial data on simvastatin in asthma: What about the rat model?

Controversial data on simvastatin in asthma: What about the rat model?

... In that report, 4.0 or 40 mg/kg body weight of the drug was administered to mice either orally or intraperitoneally (IP), leading to a reduction in inflammatory cell infiltrates and eosinophilia in bronchoalveolar ...

7

IP without IP addresses

IP without IP addresses

... the IP-level multipath capability for upper-layer protocols in a general way, rather than the protocol-specific solutions that exist in protocols such as MP-TCP ...multihomed IP connectivity as first class ...

8

Anomaly based network intrusion detection enhancement by prediction threshold adaptation of binary classification models

Anomaly based network intrusion detection enhancement by prediction threshold adaptation of binary classification models

... Number of UDP connections with source IP=current destination IP, destination IP=current source IP and source port=current source port as the current connection in a 5 second window.. Num[r] ...

354

Cost Analysis of Mobile IP for the Repetitive IP Stations during a Short Period of Time

Cost Analysis of Mobile IP for the Repetitive IP Stations during a Short Period of Time

... The MIP enables IP host mobility without breaking the high level connection. It enables a TCP connection to remain alive and to continue receiving packets when an MN moves from one point of attachment to another. ...

6

IP Telephony

IP Telephony

... (IETF). IP telephony is the exchange of information primarily in the form of speech that utilizes a mechanism known as Internet ...Protocol. IP TELEPHONY is a technique or a communication protocol which is ...

5

CIP2A mediates fibronectin-induced bladder cancer cell proliferation by stabilizing β-catenin

CIP2A mediates fibronectin-induced bladder cancer cell proliferation by stabilizing β-catenin

... CIP2A short hairpin RNA (sh-CIP2A) plasmid and CIP2A overexpressed plasmid (CIP2A-plasmid) which were ligated in pGV101 vector and pGV142 vector respectively, were purchased from Shanghai GeneChem Technologies ...

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SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL SCREENING OF NOVEL SERIES OF IMIDAZO[1,2 a]PYRIDINE DERIVATIVES LINKED TO SECONDARY AMINES

SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL SCREENING OF NOVEL SERIES OF IMIDAZO[1,2 a]PYRIDINE DERIVATIVES LINKED TO SECONDARY AMINES

... General procedure for the preparation of 2-(4-Fluorophenyl)-6-methyl-3-(N,N- dialkylamine-4-ylmethyl)imidazo[1,2-a]pyridines (IP-1 TO IP-10): To a solution of 2-(4- ...

10

Mobility as a first class function

Mobility as a first class function

... Level 3 Multihoming Shim Protocol for IPv6 (SHIM6) [29] adds a ‘shim’ layer between the network and the transport layer to separate identifier and locator from a single IP address. SHIM6 is designed for ...

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