collagen gels
Platelets stimulate fibroblast-mediated contraction of collagen gels
... of collagen gels, originally described by Bell et ...dimensional collagen gel system the ability of fibroblasts to contract the gels is dependent on a variety of factors including fibroblast ...
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Intestinal epithelial organoids fuse to form self organizing tubes in floating collagen gels
... pre-mixed collagen gel at ∼ 6000 organoids/ml and seeded in a continuous gel ring at the edges of BSA (Life Technologies, 1% w/v in PBS) blocked wells in 24-well suspension plates (Greiner) at ∼ 1200 organoids in ...
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TGF-β1 and serum both stimulate contraction but differentially affect apoptosis in 3D collagen gels
... three-dimensional collagen gels is a model of wound healing and ...I collagen gels, exposed to TGF- β 1 or FCS, and allowed to contract for 5 ...augmented collagen gel ...
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Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells
... (3D) collagen gel, but not a 3D collagen gel that is left attached to the culture ...3D collagen gels, immunostaining cellular structures, and performing biochemical procedures directly from ...
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Development and mechanical characterisation of self-compressed collagen gels
... The aims of this paper are twofold. Firstly, we present a simple technique for the slow compaction of collagen gels, increasing homogeneity and mechanical integrity. Secondly, we describe a novel approach ...
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Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture
... blast-mediated collagen gel contraction to augmented contraction is, in part, due to differentiation of monocytes within the ...3D collagen gels did not change the contractile pheno- type; for ...
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Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in three-dimensional collagen gels
... the gels were gently soaked with ...The gels were stained with ...The gels were dried directly between cellophane sheets (Pharmacia Biotech, San Francisco, CA, ...
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Development of a Platform for Studying 3D Astrocyte Mechanobiology: Compression of Astrocytes in Collagen Gels
... control and compressed collagen type I gel discs normalized to 0 hour static controls at.. “Horizontal” refers to cell alignment averaged from 4.[r] ...
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Inhibitory effect of quercetin on epithelial to mesenchymal transition in SK-MEL-28 human melanoma cells defined by in vitro analysis on 3D collagen gels
... of collagen I matrix displayed a prominent feature of EMT by forming scattered and meshwork-like morphology, which has not been previously described for SK-MEL-28 ...on collagen I matrix displayed apoptotic ...
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IL-13 induces a bronchial epithelial phenotype that is profibrotic
... time, collagen becomes insoluble due to intermolecular ...soluble collagen levels are ele- vated when the fibroblast-embedded collagen gel is co- cultured with the airway epithelium compared to ...
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Regulation of epithelial cell transformation and survival by Raf activation
... 4.11 Activation of Raf:ER in MDCK cells leads to induction of an invasive phenotype in collagen gels dependent on autocrine TGF P effects...I l l 4.12 TGFP induced cell cycle arrest i[r] ...
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Noncanonical roles of membranous lysyl-tRNA synthetase in transducing cell-substrate signaling for invasive dissemination of colon cancer spheroids in 3D collagen I gels
... KRS regulates cell-substrate adhesion during dissemination. KRS is phosphorylated at Ser207 by ERK1/2 for nuclear translocation to regulate microphthalmia-associated transcription factor (MITF)- dependent gene expression ...
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Salvermoser, Melanie (2018): Myosin 1f plays a fundamental role for neutrophil migration in 3D environments during acute inflammation. Dissertation, LMU München: Medizinische Fakultät
... 3D collagen gels, their subcellular localization was studied by spinning disk confocal microscopy using HL-60 cells stably expressing an EGFP-Myo1f fusion ...3D collagen meshwork 127 ...
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An in vitro assay of collagen fiber alignment by acupuncture needle rotation
... where collagen fibers are oriented parallel or per- pendicular to the optical axis of either polarizing element; the brightest area occurs where collagen fibers are ori- ented 45° to the filter optical ...
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The effects of nanoparticles on the physical properties of type I collagen : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Chemistry at Massey University, Palmerston North, New Zealand
... solutions before fibrillogenesis was carried out. They were found to affect the linear rheology of the collagen gels as a function of their concentration. There were no significant differences in the ...
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PubMedCentral-PMC4794404.pdf
... compact collagen bundles ...abundant collagen surrounding tumor cells and fibroblastic cells ...produce collagen and to align collagen fibers and generate stiffness in collagen ...
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Discoidin domain receptor 1 promotes Th17 cell migration by activating the RhoA/ROCK/MAPK/ERK signaling pathway
... 3D collagen has not been previously ...only collagen gel (3D) but not coated monomeric collagen (2D) activated RhoA and ROCK (Figure ...from collagen had no effect on RhoA or ROCK activity ...
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Antimicrobial Graft Copolymer Gels.
... Inhibition of bacterial growth can be achieved using the new PMPC-g-PHPMA graft copolymer gel described herein. Moreover, there is some evidence that this new copolymer architecture may be more e ff ective than the PHPMA ...
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Spatially resolved multicomponent gels
... 12 glucono-δ-lactone (GdL) and shaken gently. 1 mL was then transferred to a 20 mL plastic syringe with the nozzle removed to act as a mould. The open top of the syringe was covered with parafilm and the solution was ...
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Porosity Gradient Gels pdf
... homogeneous gels uses a number of gels of different PA concentration in the range of 4 } 35% T and estimates the relative elec- trophoretic mobility referred to Bromophenol blue (R F value) of a set of ...
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