Combing DNA from in vitro Replication Experiments
Establishing DNA combing to investigate DNA replication stress in vitro
... in replication dynamics between different batches of cells could lead to false ...different replication fork speeds on multiple occasions between studies (Técher et al, ...in experiments in exactly ...
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Replication of polyoma DNA in isolated nuclei. V. Complementation of in vitro DNA replication.
... Prelabeled depleted nuclei (13 ug of DNA) were incubated under standard conditions with [32P]-labeled dGTP for 30 min: (A) alone, (B) with DNA ligase I from calf thymus, or (C) with 50 ,[r] ...
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In vitro replication of adeno-associated virus DNA.
... (A) NE DNA was incubated with S100 extracts from either uninfected or Ad-infected HeLa cells in the presence of no additional extract, a wild-type baculovirus (wt bac) nuclear extract, o[r] ...
11
A study of the in vitro initiation of adenovirus DNA replication
... origin DNA. The perfect conservation of the sequence from base pairs 9 to 18 throughout ail human adenovirus serotypes probably reflects a crucial functional constraint on this domain, which makes it a very ...
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DNA polymerase requirements for parvovirus H-1 DNA replication in vitro.
... We have recently demonstrated the ability of purified polymerase -y to carry out the synthesis in vitro of RF from single-stranded viral DNA (1Sa), a reaction quite similar or identical [r] ...
8
Adenovirus preterminal protein synthesized in COS cells from cloned DNA is active in DNA replication in vitro.
... Expression of enzymatically active adenovirus DNA polymerase from cloned DNA requires sequences upstream of the main open reading frame. Adenovirus DNA replication in vitro: a[r] ...
5
Initiation of simian virus 40 DNA replication in vitro.
... Cosl cytoplasm containing SV40 T antigen can change the reaction of HeLa nuclear extract. from the repair to semiconservative replication[r] ...
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Role of the Adenovirus DNA-Binding Protein in In Vitro Adeno-Associated Virus DNA Replication
... extract from uninfected cells supports enhanced AAV DNA ...short replication products characteristic of displacement of the elongating strand from the ...that replication of short ...
8
Combing Chromosomal DNA Mediated by the SMC Complex: Structure and Mechanisms
... consist of a circular chromosome and possible extrachromo- somal elements. Euryarchaeal species contain multiple chromo- some copies, and harbor genes for archaeal histone homologs, whereas the crenarchaeal genera ...
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Herpes simplex virus DNA synthesis at a preformed replication fork in vitro.
... Proteins from herpes simplex virus (HSV)-infected cells were used to reconstitute DNA synthesis in vitro on a preformed replication fork.. The preformed replication fork consisted of a n[r] ...
11
In vitro replication of adeno-associated virus DNA: enhancement by extracts from adenovirus-infected HeLa cells.
... of replication apparently restarts the clock with respect to displacement; hence, with a short enough template, multiple rounds of synthesis of full-length copies of the template occur even in an uninfected cell ...
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Initiation of Human DNA Replication in Vitro Using Nuclei from Cells Arrested at an Initiation-competent State*
... nuclei from mimosine-arrested cells provides an extensive and robust sim- plification in the experimental protocols to study human initi- ation of DNA replication in ...obtained from ...
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Replication of damaged DNA
... partial replication fork structure lacked a leading strand; it is the preferred fork substrate of RecG and has been described before (McGlynn and Lloyd 2001b; Mahdi et ...the DNA substrates are detailed in ...
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Introductory Experiments in Recombinant DNA
... lambda DNA into their own genome become resistant to infection and may form small colonies within the zone of ...lambda DNA to cause the lysis of bacteria serves as the selective marker for the presence of ...
16
Identification of two distinct regions within the adenovirus minimal origin of replication that are required for adenovirus type 4 DNA replication in vitro.
... Plasmid templates (100 ng) p4A2, pHR18, and pM9 to pM18 were cleaved with EcoRI and AvaII and incubated with 3 ,ug of cytoplasmic extract from Ad4-infected HeLa cells in a standard elong[r] ...
8
Human Immunodeficiency Virus Type 1 Vpr Induces DNA Replication Stress In Vitro and In Vivo
... of DNA damage, specifically activation of the ataxia telangiectasia mutated and Rad3-related kinase, ...of replication protein A in nuclear foci, an indication that Vpr likely induces stalling of ...
12
Late replicative intermediates are accumulated during simian virus 40 DNA replication in vivo and in vitro.
... These intermediates sediment in neutral sucrose as a single peak of 29S and are seen when replicative intermediates are extracted from cells and ana- lyzed directly on alkaline gradients[r] ...
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In Vitro Whole Genome DNA Binding Analysis of the Bacterial Replication Initiator and Transcription Factor DnaA
... the DNA binding protein, with DNA-protein complexes recovered by metal affinity ...preliminary experiments, we found that filter binding, which does not require a tag, can also be used to recover ...
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Intermediates of adeno-associated virus DNA replication in vitro.
... replication of parvovirus and linear chromosomal DNA. In vitro rescue of an integrated hybrid adeno-associated virus/simian virus 40) genome.[r] ...
5
In vitro termination of adenovirus DNA synthesis by a soluble replication complex.
... DNA in the replication complex was labeled in vitro for 20 min with [3H]dTTP, then purified and digested with restriction enzyme (after addition of uniformly labeled Ad2 /'4C]DNA), and d[r] ...
5