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Complementary DNA

Fractionation of DNA nucleotide transcripts from Moloney sarcoma virus and isolation of sarcoma virus-specific complementary DNA.

Fractionation of DNA nucleotide transcripts from Moloney sarcoma virus and isolation of sarcoma virus-specific complementary DNA.

... In this manner three sets of nucleotide sequences which represent different portions of the MSV viral complex were obtained: a sarcoma virus-specific fraction cDNAa, with sequences that [r] ...

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Isolation of complementary DNA for bullous pemphigoid antigen by use of patients' autoantibodies

Isolation of complementary DNA for bullous pemphigoid antigen by use of patients' autoantibodies

... complementary riboprobes transcribed from both fragments hybridized to a 9-kb RNA. Dideoxy DNA sequencing from the 5' end of the BP cDNA demonstrated a 1,992-bp open reading frame, encoding a peptide of 76 ...

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Cloning and characterization of complementary DNA for human tryptase

Cloning and characterization of complementary DNA for human tryptase

... Asp2" , which is substituted for the Gly found in dog tryptase and in most serine proteases, is present in the putative substrate binding pocket and may confer additional substrate speci[r] ...

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Cloning and characterization of a second complementary DNA for human tryptase

Cloning and characterization of a second complementary DNA for human tryptase

... Catalytic triad residues ***, leader sequence -, putative substrate binding pocket regions - , and the putative asparagine-linked carbohydrate binding site are shown for the amino acid[r] ...

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Dual nanoparticle amplified surface plasmon resonance detection of thrombin at subattomolar concentrations

Dual nanoparticle amplified surface plasmon resonance detection of thrombin at subattomolar concentrations

... a starting point for developing the amplification approach described here. This involves appending a polyadenine tail to the aptamer probe sequence as a platform for the sequential hybridization adsorption of ...

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Mediated Electron Transfer at Vertically Aligned Single Walled Carbon Nanotube Electrodes During Detection of DNA Hybridization

Mediated Electron Transfer at Vertically Aligned Single Walled Carbon Nanotube Electrodes During Detection of DNA Hybridization

... tential DNA biosensor ...terminated DNA is coupled to the free end of the aligned ...the complementary DNA strands in solution is followed using ...

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A novel Babesia orientalis 135 kilodalton spherical body protein like: identification of its secretion into cytoplasm of infected erythrocytes

A novel Babesia orientalis 135 kilodalton spherical body protein like: identification of its secretion into cytoplasm of infected erythrocytes

... Abbreviations AMA-1: Apical membrane antigen-1; BoSBP3-like: The SBP3-like in Babesia orientalis; cDNA: Complementary DNA; CDS: Sequence coding for amino acids; gDNA: Genomic DNA; GPI: G[r] ...

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Nanomechanical sensing of hypermethylated DNA for the detection of bladder cancer

Nanomechanical sensing of hypermethylated DNA for the detection of bladder cancer

... researcher of the IMS group, predict a frequency shift of − 31.2 ppm / K for a bare silicon cantilever. Besides, many material properties are known to change with temperature. Thermal mismatch between different materials ...

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A Highly Sensitive Electrochemical DNA Biosensor from Acrylic Gold Nano composite for the Determination of Arowana Fish Gender

A Highly Sensitive Electrochemical DNA Biosensor from Acrylic Gold Nano composite for the Determination of Arowana Fish Gender

... of DNA probe was diluted with ...while complementary DNA (cDNA) and non-complementary (ncDNA) solutions were prepared with ...maximum DNA probe immobilisation on the ...

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Rifampin inhibition of bacteriophage phiX174 parental replicative-form DNA synthesis in an Escherichia coli dnaC mutant.

Rifampin inhibition of bacteriophage phiX174 parental replicative-form DNA synthesis in an Escherichia coli dnaC mutant.

... DISCUSSION These data show that the host dnaC protein is required for OX174 parental RF DNA synthesis, presumably in the initiation of the synthesis of the complementary DNA strand, when[r] ...

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Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis

Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis

... cDNA: complementary DNA; Chik1: chikungunya virus, strain PR-S6 sample; CHIKV: chikungunya virus; DNA: deoxyribonucleic acid; DRC: Democratic Republic of the Congo; Ebola1: Ebola virus, strain ...

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Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells

Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells

... cDNA: Complementary DNA; cRNA: Complementary RNA; CT: Computed tomography; FABP4: Fatty acid-binding protein 4; Flt3L: Fms-related tyrosine kinase 3 ligand; GAPDH: Glyceraldehyde-3-phosphate ...

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The expression of COX-2, hTERT, MDM2, LATS2 and S100A2 in different types of non-small cell lung cancer (NSCLC)

The expression of COX-2, hTERT, MDM2, LATS2 and S100A2 in different types of non-small cell lung cancer (NSCLC)

... Abbreviations used: ADC – adenocarcinoma; CDK2 – cyclin-dependent kinase 2; cDNA – complementary DNA; COX-2 – cyclooxygenase 2; CT – cycle threshold; DNA – deoxyribonucleic acid; GAPDH[r] ...

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BACTERIOPHAGE T4 GENE 32 PARTICIPATES IN EXCISION REPAIR AS WELL AS RECOMBINATIONAL REPAIR OF UV DAMAGES

BACTERIOPHAGE T4 GENE 32 PARTICIPATES IN EXCISION REPAIR AS WELL AS RECOMBINATIONAL REPAIR OF UV DAMAGES

... Specifi- cally, defects in pairing of complementary DNA strands (of different paren- tage) across UV damages would reduce potential repair of damaged DNA by recomb[r] ...

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A Compact Functional Quantum Dot-DNA Conjugate: Preparation, Hybridization and Specific Label-free DNA Detection

A Compact Functional Quantum Dot-DNA Conjugate: Preparation, Hybridization and Specific Label-free DNA Detection

... 594)-labeled DNA complementary to DNA-T brings the fluorophore in close proximity to the QD, so when the QD is excited, it efficiently undergoes energy transfer to the fluorophore via FRET, producing ...

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Diagnosis of Sugarcane White Leaf Disease Using the Highly Sensitive DNA Based  Voltammetric Electrochemical  Determination

Diagnosis of Sugarcane White Leaf Disease Using the Highly Sensitive DNA Based Voltammetric Electrochemical Determination

... the DNA grooves and therefore an extent of electrochemical responses variation could be observed ...with complementary DNA from SCWL-infected plants yields the lowest MB signal peak currents which ...

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Towards HIV Detection: Novel Poly(propylene imine) Dendrimer-Streptavidin Platform for Electrochemical DNA and gp120 Aptamer Biosensors

Towards HIV Detection: Novel Poly(propylene imine) Dendrimer-Streptavidin Platform for Electrochemical DNA and gp120 Aptamer Biosensors

... The biosensor was prepared by the immobilisation of single stranded 5'- biotynilated probe DNA and B40 aptamer on the modified electrode and then used to detect complementary DNA and gp1[r] ...

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Purification of DNA complementary to the env gene of avian sarcoma virus and analysis of relationships among the env genes of avian leukosis-sarcoma viruses.

Purification of DNA complementary to the env gene of avian sarcoma virus and analysis of relationships among the env genes of avian leukosis-sarcoma viruses.

... We have purified radioactive DNA cDNAp complementary to at least a portion of the env gene for viral subgroups A and C; complementary DNA was synthesized with purified virions of wild-ty[r] ...

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Complementary strands of CELO virus DNA.

Complementary strands of CELO virus DNA.

... Each strand would have terminal sequences of the type ABCD and D'C'B'A' where A' is complementary to A, etc., and the single-stranded circles formed by annealing would have a projecting [r] ...

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DNA and RNA analyses in detection of genetic predisposition to cancer

DNA and RNA analyses in detection of genetic predisposition to cancer

... At present, the best and the most frequently applied technique of initial detection of changes is DHPLC [5, 7-10]. This is a kind of HET based on high resolution of modern chromatographic columns. Analysed DNA ...

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