Constructs used for bimolecular fluorescence complementation
Dimerization of ABCG2 analysed by bimolecular fluorescence complementation
... a bimolecular fluorescence complementation approach to examine ABCG2 dimers, and to probe the role of individual amino acid substitutions in dimer ...by fluorescence microscopy and flow ...of ...
9
Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay
... the complementation between split Venus fragments, living embryos were dechorio- nated and mounted in the halocarbon oil 10S (commer- cialized by VWR, Pennsylvania, ...dermal fluorescence were taken in ...
18
Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry
... sion constructs from singular colonies lead to positive BiFC events with the bait construct were recovered: 10 out of the 37 had positive BiFC signals, meaning that 27% had encoded fusion proteins putatively ...
17
Detection and Characterization of Influenza A Virus PA-PB2 Interaction through a Bimolecular Fluorescence Complementation Assay
... utilized bimolecular fluorescence complementation (BiFC) to study protein-protein interactions in the assembly of the influenza A virus polymerase ...
12
MCM GINS and MCM MCM interactions in vivo visualised by bimolecular fluorescence complementation in fission yeast
... have used BiFC for the first time in fission yeast to demonstrate interactions between the GINS and MCM complexes in the nuclei of exponentially-growing cells and on chromatin in S-phase cells and when replica- ...
7
Use of Bimolecular Fluorescence Complementation To Study In Vivo Interactions between Cdc42p and Rdi1p of Saccharomyces cerevisiae
... DNA manipulations. p415MET(YN-RDI1) and p416MET(CC-CDC42) were made by D-loop PCR utilizing primers (Invitrogen, Carlsbad, CA) that removed amino acids (aa) 155 to 238 from YFP in p415MET(YFP-RDI1) (46) and aa 1 to 154 ...
10
Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells
... yellow fluorescence, respectively, than using other GFP- derived autofluorescent protein ...therefore used nVenus or nCerulean fragments from amino acids 1–173, and cCFP from amino acids ...
11
Identification of Transmembrane Protein 134 as a Novel LMP1-Binding Protein by Using Bimolecular Fluorescence Complementation and an Enhanced Retroviral Mutagen
... were used to establish gating for BiFC-positive cells that excluded BiFC-negative ...by fluorescence-acti- vated cell sorting (FACS) using the FACS Aria (BD Biosciences) in the Rosalind Franklin University ...
11
Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of β arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors
... were used for excitation, and emitted light collected via a 505/550 nm bandpass fi ...were used for images of control and ag- onist treated cells within the same ...
14
Characterization of the latent membrane protein 1 signaling complex of Epstein Barr virus in the membrane of mammalian cells with bimolecular fluorescence complementation
... rigorous fluorescence techniques like fluorescence resonance energy transfer ...high fluorescence represent likely combinations for positions for FRET ...
15
Distinct Intracellular Trafficking of Equine Infectious Anemia Virus and Human Immunodeficiency Virus Type 1 Gag during Viral Assembly and Budding Revealed by Bimolecular Fluorescence Complementation Assays
... we used bimolecular fluorescence complementation to charac- terize and compare assembly sites and budding efficiencies of EIAV and HIV-1 Gag in both human and rodent ...[RRE]) used by ...
10
Bimolecular Complementation of Paramyxovirus Fusion and Hemagglutinin-Neuraminidase Proteins Enhances Fusion: Implications for the Mechanism of Fusion Triggering
... by bimolecular fluorescence complementation (BiFC), the cytoplasmic tails of parainfluenza virus 5 (PIV5) F and HN were fused to complementary fragments of yellow fluorescent protein ...BiFC ...
12
Bimolecular Complementation Defines Functional Regions of Herpes Simplex Virus gB That Are Involved with gH/gL as a Necessary Step Leading to Cell Fusion
... terest brings the two halves of the fluorophore in close enough contact to restore fluorescence. When HSV receptor-bearing cells, such as B78H1 cells that are engineered to express nectin-1, are transfected with ...
10
Screening for Novel Small-Molecule Inhibitors Targeting the Assembly of Influenza Virus Polymerase Complex by a Bimolecular Luminescence Complementation-Based Reporter System
... Here we developed the Tet on-BiLC assay to detect the influenza virus polymerase assembly and to screen novel therapeutics methods that inhibit influenza virus poly- merase assembly. By combining in silico modeling data, ...
13
High Precision Fret to Study Bimolecular Dynamics with High Temporal Resolution
... be used to derive distances for distance-restrained structural modeling with high ...of fluorescence information such as excitation and emission spectrum, anisotropy, fluorescence lifetime, quantum ...
110
Proton Impact of Bimolecular Targets
... been used to calculate the probability for an energetic proton and to produce electronic excitations in five targets of high biological interest, namely, liquid water, DNA, PMMA, Adenine and ...
11
Assessment of the fluorescence and auger database used in plasma modeling
... basis used in this region, we recommend that if scaling along an isoelectronic sequence is to be performed, the better starting point would be at the highest Z desired, extrapolating the rates to lower Z ...the ...
19
Supplemental Fig. S1. The schematic diagrams of the expression constructs used in this study.
... The reaction, with the caspase-inhibitor Z-VAD-FMK added, was incubated for 24 hrs. Numbers below the blots show the ratio of full-length protein density at each time point to that at the 0 time point. Supplemental Fig. ...
13
Constructs of Freedom
... The function of a Legislature of a Colony is to aid and advise the governor, who is the government, and the Cabinet is to administer affairs in any department to which he is appointed [r] ...
26
Complementation in finite groups
... Chapter 2 is an investigation of a complementation problem, namely the question of finite groups with the property that all the pro normal subgroups have compleme[r] ...
138