control shRNA
Overexpression of HOXC10 promotes angiogenesis in human glioma via interaction with PRMT5 and upregulation of VEGFA expression
... Previous studies have suggested that the expression of VEGFA is biologically and clinically involved in the angiogenesis of tumor cells [35]. Interestingly, three potential HOXC10 binding sites were predicted in the ...
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ROCK1 and LIM Kinase Modulate Retrovirus Particle Release and Cell-Cell Transmission Events
... Electron microscopy. HeLa cells stably expressing control shRNA or LIMK1 shRNA were transfected with NL4-3 provirus and cultured in 6-well plates. Forty-eight hours following transfection, cells were ...
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THE ROLE OF PLATELET ENDOTHELIAL AGGREGATION RECEPTOR 1 IN MIGRATION, ADHESION, AND PROLIFERATION
... or control shRNA-expressing lentiviral vectors to fibronectin, collagen I, collagen IV, laminin I, fibrinogen, and bovine serum albumin (BSA) were evaluated in 6 independent cell ...expressing ...
10
<p>Knockdown Of lncRNA NCK-AS1 Regulates Cisplatin Resistance Through Modulating miR-137 In Osteosarcoma Cells</p>
... attenuating cell viability and abolishing the activities of proliferation-related proteins CDK2 and cyclinE1, repressed cell invasion and migration, and suppressed the protein expression of MMP9 and MMP13 in parental and ...
12
Loss of HSulf-1 promotes altered lipid metabolism in ovarian cancer
... HSulf-1 shRNA-silenced cells (Sh1 and Sh2 cells) compared to non-targeted control shRNA cells (NTC cells) and subsequent Kyoto Encyclopedia of Genes and Genomics (KEGG) database analysis showed ...
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<p>Effect of <em>SMYD3</em> on biological behavior and H3K4 methylation in bladder cancer</p>
... The shRNA-SMYD3-3 group exhibited signi fi cantly lower proliferation rate and was negatively correlated with time than the control and shRNA-NC ...in shRNA-NC were ...with control or ...
9
PP4R1 accelerates cell growth and proliferation in HepG2 hepatocellular carcinoma
... Con, control; GFP, green fluorescent protein; Lv-shCon, control shRNA; Lv-shPP4R1, lentivirus expressing PP4R1 shRNA; OD, optical density; PP4R1, protein phosphatase 4 regulatory subunit 1; ...
8
LINC01503/miR-342-3p facilitates malignancy in non-small-cell lung cancer cells via regulating LASP1
... specific shRNA targeting LINC01503 (sh- LINC01503) and negative control shRNA (sh-NC) were used in ...the control groups ...LINC01503 shRNA transfection group com- pared to the negative ...
12
SPARC regulates transforming growth factor beta induced (TGFBI) extracellular matrix deposition and paclitaxel response in ovarian cancer cells
... separate shRNA targets against SPARC by Lentivirus ...SPARC shRNA cells for a period of 9 days, cells were denuded from the deposited matrix and the ECM was analyzed for TGFBI expression by ...
20
Induction of metastatic potential by TrkB via activation of IL6/ JAK2/STAT3 and PI3K/AKT signaling in breast cancer
... TrkB- shRNA partially suppressed the expression of endogenous TrkB ...MDA-MB-231 control-shRNA or TrkB-shRNA ...MDA-MB-231 control-shRNA cells (Figure ...
14
Original Article Silencing Bmi1 inhibits the proliferation and invasiveness in CD133 cancer stem cells of Hep-2 cells
... cancer stem cells are derived from original tis- sue-derived stem cells, bone marrow stem cells, or mature cells that have undergone a Figure 3. Bmi1 silencing decreased cell invasion, the cell invasion as- say and ...
8
Modulation of anti-cancer drug sensitivity through the regulation of mitochondrial activity by adenylate kinase 4
... positive control, ATP5a, which is one of ATP synthase subunits and a mitochondrial marker, but not as hexoki- nase 2 (HK2), which associates with both mitochondrial and cytoplasmic compartments, while HK2 ...
15
Elongation factor 1A-1 and hepatocyte response to fatty acid excess
... the control and accuracy of peptide ...quality control of newly-synthesized proteins by binding nascent or unfolded proteins that are no longer associated with the ribosome, but not to correctly folded ...
115
The Innate Immune Factor Apolipoprotein L1 Restricts HIV-1 Infection
... H uman apolipoprotein L1 (APOL1) is the product of a mem- ber of a family of six APOL genes grouped on chromosome 22 in the region encompassing bands q12.3 to q13.1 (1–4). Interest- ingly, the APOL1 gene is located in ...
12
Neutralizing Antibody-Resistant Hepatitis C Virus Cell-to-Cell Transmission
... FIG. 4. HCV coculture transmission is CD81 dependent. (A) Anti-CD81 inhibition of H77/JFH coculture transmission. The stacked histogram displays nAb-resistant cell-to-cell (open portion of bar) and nAb-sensitive (filled ...
10
SC79 protects retinal pigment epithelium cells from UV radiation via activating Akt-Nrf2 signaling
... To rule out the possible off-target effect of MK-2206, we utilized targeted shRNA to selectively knockdown Akt1 in ARPE-19 cells (See the same protocol in our previous study [6]). SC79-induced Akt activation was ...
10
Osteopontin mediates glioblastoma associated macrophage infiltration and is a potential therapeutic target
... untreated control (22 days), 4-1BB aptamer (27 days), OPN aptamer (26 days), or a mixture of the 4-1BB and OPN aptamers (23 days) (n = 5 mice/group) (P < ...A control aptamer that does not target the ...
14
Snail promotes metastasis of nasopharyngeal carcinoma partly by down-regulating TEL2
... Cell lines stably expressing short hairpin RNA (shRNA) targeting Snail transcripts or negative control scram- bled shRNA were established using kits from Sigma. The sequences of the 2 human Snail ...
10
BCYRN1, a c-MYC-activated long non-coding RNA, regulates cell metastasis of non-small-cell lung cancer
... After the A549 cells were treated with c-MYC inhibitor 10058-F4, or infected with pWPXL-c-MYC or pWPXL- NC, or transfected with siRNA-NC, siRNA-BCYRN1-1 or siRNA-BCYRN1-2, the medium was replaced with serum-free medium. ...
8
Multiple shRNA combinations for near-complete coverage of all HIV-1 strains
... single shRNA, combinations, and non-matched con- trol plasmids were separately transfected with all three reporters (Figure 7a) (Additional file 1 for the control ...single shRNA differed slightly in ...
15