COS-7
Archetype JC Virus Efficiently Replicates in COS-7 Cells, Simian Cells Constitutively Expressing Simian Virus 40 T Antigen
... in COS-7 cells, since the arche- type generates various PML-type regulatory regions by dele- tion and amplification during the persistence of JCV in patients (3, 9, 20, 21, 45, ...infected ...
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COS 7 based model: methodological approach to study John Cunningham virus replication cycle
... 6 COS-7 ...for COS-7 cells and as genome equivalents (gEq) of viral DNA per milliliter (gEq/ml) for ...supernatants. COS-7 cells and supernatants, collected one time a week until ...
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Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin Dependent Methionine Synthase in Mammalian Cultured COS 7 Cells
... Figure 5. Effects of the addition of dodecylamine and c-lactam derivatives of OH-Cbl on MS activity of COS-7 cells. Total (a) and holo- (b) MS activities were assayed in the cell homo- genates of ...
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Lack of hormone binding in COS 7 cells expressing a mutated growth hormone receptor found in Laron dwarfism
... A single point mutation in the growth hormone (GH) receptor gene generating a Phe-->Ser substitution in the extracellular binding domain of the receptor has been identified in one family with Laron type dwarfism. The ...
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Absence of Proton Channels in COS-7 Cells Expressing Functional NADPH Oxidase Components
... The main function of voltage-gated proton channels in phagocytes is thought to be to provide a mechanism of charge compensation for the electron efflux through NADPH oxidase (Henderson et al., 1987; Eder and De- Coursey, ...
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Transcription of the cottontail rabbit papillomavirus early region and identification of two E6 polypeptides in COS-7 cells.
... First, E6 in BPV-1 was shown to code for a transforming protein, and second, unlike BPV-1, mapping of viral transcripts from CRPV-induced tumors strongly suggests that two different E6 p[r] ...
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Muscarinic receptor activation of the MAP kinase signalling pathway and its physiological consequences
... in COS-7 and CHO cells was dependent on Gp^ subunits, Ras, Raf and protein tyrosine kinases, whilst the Gq-mediated pathway was dependent on G^^q, Raf and PKC (Hawes et a l, 1995; Lutterell et a l, ...
255
Anti-infective and cytotoxic properties of Bupleurum marginatum
... kidney COS-7 (fibro- blast-like epithelium) and CCL-81 (Vero) cell lines were maintained in DMEM complete medium (L-glutamine supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin, ...
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Examination of actin and microtubule dependent APC localisations in living mammalian cells
... workers have presented an examination of GFP-APC dynamic behaviour in transfected COS-7 cells [11] but did not report findings similar to those presented here. The reasons for this are unclear. Although the ...
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Membrane Association and Dimerization of a Cysteine-Rich, 16-Kilodalton Polypeptide Released from the C-Terminal Region of the Coronavirus Infectious Bronchitis Virus 1a Polyprotein
... of Cos-7 cells expressing the T7-tagged 16-kDa protein with anti-T7 monoclonal antibody (1:200) at 6 h posttransfection showed positive staining under nonpermeabilized conditions (A) and a perinuclear ...
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Pacemaker channel dysfunction in a patient with sinus node disease
... domain. COS-7 cells transient- ly transfected with HCN4-573X cDNA indicated normal intracellular trafficking and membrane inte- gration of HCN4-573X ...
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Mitogen activated protein kinase inhibits 1,25 dihydroxyvitamin D3–dependent signal transduction by phosphorylating human retinoid X receptor α
... In vitro kinase assay. COS-7 cells were grown in DMEM supple- mented with 10% FBS. Transfections were performed by incubat- ing 5 µg of plasmid DNA expressing the human vitamin D recep- tor (hVDR) (a kind ...
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Construction of the Chimeric HSP70 - E7 Vector and Evaluation of its Protein Production
... Methods: According to the known Immunomodulatory effect and structure of HSP70 molecule, full length human HSP70 was selected and a hybrid was made with HPV- E7gene.The recombinant plasmid pCDNA3.1/ E7-HSP70 was ...
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Neumann Functions and Its Application for Finding The Solution to the Schrödinger’s equation in a Cylindrical Well
... Bessel function of the second kind is known as Neumann functions, are developed as a linear combination of Bessel function of the first order described: − cos = 7 sin For integral va[r] ...
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An investigation of the binding of human monoclonal antibodies to autoantigens by the modification and expression of cloned autoantibody cDNA
... the COS-7 cell supernatants from ...f COS-7 cell supernatants that were not DNasel-treated were too low to be detected by the ELISA and are therefore not shown in the ...from ...
303
Gipc1 has a dual role in Vangl2 trafficking and hair bundle integrity in the inner ear
... of COS-7 cells transiently transfected with DsRed-Vangl2, myc-Gipc1 or a combination of the constructs, and labeled with phalloidin (blue), with a line scan corresponding to the dashed line for each ...
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Fematrin-1 Is Involved in Fetomaternal Cell-to-Cell Fusion in Bovinae Placenta and Has Contributed to Diversity of Ruminant Placentation
... spekii. Cos-7 cell lysates transfected with expression vectors carrying Fematrin-1 from each species were electrophoresed and then immunoblotted by using an anti-BK1SU ...
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Structural and functional analysis of muskelin.
... A number of natural disease-causing mutations in the kelch-repeat proteins RAG-2 and gigaxonin involve non-conservative substitutions of single residues within the kelch repeats. These are thought to disrupt the ...
278
Assessment of total, ligand-induced peroxisome proliferator activated receptor γ ligand activity in serum
... in Cos-7 cells, the cell model for the reporter assay. Cos-7 cells endogenously express PPAR γ and RXRs, the receptors necessary to support ligand-induced PPAR γ activity (column 1 (left ...
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Two Proline-Rich Nuclear Localization Signals in the Amino- and Carboxyl-Terminal Regions of the Borna Disease Virus Phosphoprotein
... The transfected cells were also subjected to indirect immunofluorescence assay (IFA). After culture, the cells were fixed with 4% paraformaldehyde prior to treatment with 0.4% Triton X-100 (32). To detect BDV P and its ...
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