COS-7 cells
Archetype JC Virus Efficiently Replicates in COS-7 Cells, Simian Cells Constitutively Expressing Simian Virus 40 T Antigen
... POJ cells. The replication of archetype JCV in POJ cells was detected by the continuous presence of faint hybridization signals during the incubation of transfected ...POJ cells than in ...
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Dodecylamine Derivative of Hydroxocobalamin Acts as a Potent Inhibitor of Cobalamin Dependent Methionine Synthase in Mammalian Cultured COS 7 Cells
... the COS-7 cells grown in ...control cells, respectively) than the hexylamine ...cultured COS-7 under the experimental ...
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Lack of hormone binding in COS 7 cells expressing a mutated growth hormone receptor found in Laron dwarfism
... A single point mutation in the growth hormone (GH) receptor gene generating a Phe-->Ser substitution in the extracellular binding domain of the receptor has been identified in one family with Laron type dwarfism. The ...
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Absence of Proton Channels in COS-7 Cells Expressing Functional NADPH Oxidase Components
... The main function of voltage-gated proton channels in phagocytes is thought to be to provide a mechanism of charge compensation for the electron efflux through NADPH oxidase (Henderson et al., 1987; Eder and De- Coursey, ...
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Transcription of the cottontail rabbit papillomavirus early region and identification of two E6 polypeptides in COS-7 cells.
... First, E6 in BPV-1 was shown to code for a transforming protein, and second, unlike BPV-1, mapping of viral transcripts from CRPV-induced tumors strongly suggests that two different E6 p[r] ...
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Membrane Association and Dimerization of a Cysteine-Rich, 16-Kilodalton Polypeptide Released from the C-Terminal Region of the Coronavirus Infectious Bronchitis Virus 1a Polyprotein
... BHK-21 cells with helper RNA, which encodes the Sindbis virus structural pro- teins for the assembly of ...the cells transfected with the GFP construct produced the protein, cells were starved in ...
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Simian virus 40-transformed human cells that express large T antigens defective for viral DNA replication.
... Although the fragment encoded a full-length viral early region and could induce T antigen effectively in CV-1 cells, its replication was only detected when transfected into COS-7 cells, [r] ...
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Pacemaker channel dysfunction in a patient with sinus node disease
... an altered sensitivity to changes in intracellular cAMP concentrations. Since the 1631delC mutation in the index patient’s HCN4 gene was heterozygous, we also simulated the heterozygous condition by coexpressing HCN4 and ...
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Multiple Effects of Codon Usage Optimization on Expression and Immunogenicity of DNA Candidate Vaccines Encoding the Human Immunodeficiency Virus Type 1 Gag Protein
... We have analyzed the influence of codon usage modifications on the expression levels and immunogenicity of DNA vaccines, encoding the human immunodeficiency virus type 1 (HIV-1) group-specific antigen (Gag). In the ...
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Effect of Calcium-Modulating Cyclophilin Ligand on Human Immunodeficiency Virus Type 1 Particle Release and Cell Surface Expression of Tetherin
... permissive Cos-7 ...in Cos-7 ...monkey Cos-7 cells in the presence or absence of Vpu ...using Cos-7 cells after transfection with HxBH10vpu_wt or ...
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Gipc1 has a dual role in Vangl2 trafficking and hair bundle integrity in the inner ear
... of COS-7 cells transiently transfected with DsRed-Vangl2, myc-Gipc1 or a combination of the constructs, and labeled with phalloidin (blue), with a line scan corresponding to the dashed line for each ...
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Examination of actin and microtubule dependent APC localisations in living mammalian cells
... in cells imaged in subconfluent cultures although other minor localisations were also ...fixed COS-7 cells expressing GFP-APC co-stained with γ-tubulin (Figure 5, panels ...of cells a ...
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Reconstitution of mutant V2 vasopressin receptors by adenovirus mediated gene transfer Molecular basis and clinical implication
... from COS-7 cells transfected with E242stop or V2-tail alone or mixed after solubilization did not result in OD readings that were signifi- cantly higher than background, even when the sandwich ELISA ...
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Mitogen activated protein kinase inhibits 1,25 dihydroxyvitamin D3–dependent signal transduction by phosphorylating human retinoid X receptor α
... assay. COS-7 cells were grown in DMEM supple- mented with 10% ...transfected cells were harvested for gel retardation assays 48 hours after transfection by washing the cells twice in ...
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Two Proline-Rich Nuclear Localization Signals in the Amino- and Carboxyl-Terminal Regions of the Borna Disease Virus Phosphoprotein
... of cells transfected with each plasmid construct ...2). COS-7 cells transfected with pRSV-LacZ served as a positive control for staining with the MAb used ...the cells transfected with ...
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Muscarinic receptor activation of the MAP kinase signalling pathway and its physiological consequences
... In cells of the immune system; human neutrophils (Ptasznik et a l, 1995), and avian B cells (Wan et a l, 1996), Lyn and Syk are necessary for ERK ...deficient cells to demonstrate that Syk and Btk ...
255
Human Herpesvirus 6 Open Reading Frame U83 Encodes a Functional Chemokine
... mononuclear cells in ...infected cells both indicated that the encoded protein was a late ...in COS-7 cells by transfection, and the fusion protein was purified from the supernatant of ...
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COS 7 based model: methodological approach to study John Cunningham virus replication cycle
... the cells were washed 3 times with PBS and incubated with fresh medium for 4 days and then transferred into 90-mm-diameter ...the cells were continuously cultured in the maintenance medium and split at 1:5 ...
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Fematrin-1 Is Involved in Fetomaternal Cell-to-Cell Fusion in Bovinae Placenta and Has Contributed to Diversity of Ruminant Placentation
... into Cos-7 ...(B) Cos-7 cell lysates transfected with each construct were subjected to SDS-PAGE followed by blotting onto PVDF ...in Cos-7 cells transfected with ...
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Dissociation of a MAVS/IPS-1/VISA/Cardif-IKKε Molecular Complex from the Mitochondrial Outer Membrane by Hepatitis C Virus NS3-4A Proteolytic Cleavage
... A549 cells results in the induction and recruitment of IKKε to the mitochondrial membrane, in association with K1271 ...NS3-4A. COS-7 cells transfected with IKKε and K1271 expression plasmids, ...
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