Cre/loxP recombination system
Conditional gene targeting
... of loxP-flanked genes or gene seg- ments can be achieved by crossing into the mutant animal a Cre-transgene from which Cre recombinase is expressed in a cell-type–specific or inducible manner ...a ...
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Transgene Coplacement and High Efficiency Site-Specific Recombination With the Cre/loxP System in Drosophila
... ORBAN, P. BAKER, 1995 A system for inser- tional mutagenesis and chromosomal rearrangement using the Ds transposon and Cre-lox. GEWR, 1992 Position-indepen- dent germlin[r] ...
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Site-Specific Integration of an Adeno-Associated Virus Vector Plasmid Mediated by Regulated Expression of Rep Based on Cre-loxP Recombination
... of Cre-loxP recombination in a novel system for the regulated, transient expression of Rep78, which is potentially cytotoxic when synthesized con- ...However, Cre circularizes the rep ...
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Inhibition of recombinant human immunodeficiency virus type 1 replication by a site-specific recombinase.
... modified Cre could provide a reagent for therapy against HIV-1 ...cells. Cre has been shown to mediate recombi- nation between sites whose sequence diverges from canonical ...and recombination exists ...
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Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
... a system for the rapid and efficient excision of the BAC sequence from the PRV genome ...with loxP sites and adding within the BAC vector sequence an expression cassette for a Cre-encoding gene which ...
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Construction and Use of a Recyclable Marker To Examine the Role of Major Facilitator Superfamily Protein Members in Candida glabrata Drug Resistance Phenotypes
... the Cre-loxP-based recombination, which has been used extensively in a wide variety of genetic systems to remove chromosome segments of ...of Cre, the methionine-regulatable promoter (MET3) is ...
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Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression.
... The system that we have developed might be exploited for the regulated expression of toxic gene products in cells or ...This system should allow production of toxic proteins at levels sufficient for their ...
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A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme
... By Cre-loxP recombination, the conventional single-colored loxP reporter system, such as Rosa26-LacZ, Rosa26- tdTomato or Rosa26-GFP, can be used to label Cre-expressing cells ...
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Development of high-titer retroviral producer cell lines by using Cre-mediated recombination.
... P1 recombination system in the design of retroviral ...tion system comprising the phage-encoded recombinase Cre and a site on the phage genome, loxP, where recombination takes ...
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Viral Cre-LoxP tools aid genome engineering in mammalian cells
... two LoxP sites, the Cre recombinase recognizes the lox P sites and cuts ...the LoxP sites determine whether Cre recombination induces a deletion, inversion, or chromosomal transloca- ...
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A novel in vitro model for the assessment of postnatal myonuclear accretion
... by recombination, transcrip- tion, and translation of the reporter ...accretion, Cre/LoxP-based assessment does not provide any insight in the intermediate morphological states of myoblasts before ...
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Cardiac hypertrophy with preserved contractile function after selective deletion of GLUT4 from the heart
... The phenotype of GLUT4-null mice, in which GLUT4 expression was ablated in all tissues in which it is nor- mally expressed (heart, skeletal muscle, and adipose tis- sue), provided additional evidence that changes in the ...
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Roles for Dicer1 in the patterning and differentiation of the optic cup neuroepithelium
... model system with which to investigate early patterning, gradual specification and subsequent differentiation into the mature cell types (Yang, 2004; Esteve and Bovolenta, ...
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Construction of adenovirus vectors through Cre-lox recombination.
... making Cre recombinase, over 90% of the molecules were recombined, and the yield of C5 virus was about 10% of the yield of the unselected C5 ...circular recombination product was present at a lower ...
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Self-reactive B cells in the GALT are actively curtailed to prevent gut inflammation
... immune system to discriminate between harmful versus innocuous antigens to restrain the spread of pathogenic microbes, while also avoiding aberrant inflammatory encoun- ters (4, ...immune system is an ...
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Inactivation of focal adhesion kinase in cardiomyocytes promotes eccentric cardiac hypertrophy and fibrosis in mice
... Cardiac hypertrophic responses upon angiotensin II stimulation in CFKO mice. Heart and liver were harvested at 14 days after Ang II infusion (A, C, and E) or 10 days after TAC (B, D, and F), as described in Methods. ...
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Dnmt1 in Intestinal Development and Cancer
... Adult Dnmt1-ablated crypt cells maintain methylation of DNA damage response genes Given the opposing phenotypes we describe above for the neonatal intestine versus that observed in mice with Dnmt1 ablation in the adult ...
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Morphogenesis of the right ventricle requires myocardial expression of Gata4
... Conclusion. Using Cre/loxP technology we have selectively inac- tivated Gata4 within the myocardium at early and late stages of heart development. At both time points, we demonstrated that Gata4 was an ...
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Autophagy regulating TP53INP2 mediates muscle wasting and is repressed in diabetes
... Skeletal muscle mass is determined by a fine balance between protein synthesis and degradation (1). Alterations in any of these processes can result in muscle atrophy when there is a net increase in the protein ...
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Molecular dissection of integrin signalling proteins in the control of mammary epithelial development and differentiation
... or Cre (green), STAT-5 (red) and DAPI (blue) is on the right; double-headed arrows show either Ad-lacZ-infected (top panels) or Cre-negative cells (bottom panels), where STAT5 translocates to nuclei; the ...
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