CRISPR/Cas9 system
Crispr/Cas9 System - A Revolution in Gene Editing
... the CRISPR-Cas9 system to correct genetic disease ...used CRISPR-Cas9 gene editing to correct a disease-causing mutation in dozens of viable human ...with Cas9 protein, sgRNA and ...
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Applications of the CRISPR–Cas9 system in cancer biology
... the CRISPR-Cas9 system can be utilized to simultaneously disrupt up to eight alleles in mouse ES cells in a single step 38 ...their CRISPR-Cas9 methods for rapidly generating mice ...
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WU CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
... public CRISPR dataset and discovered many novel features that are characteristic of func- tional ...of CRISPR/Cas9 are also correlated to both sequence and structural features of the ...the ...
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The CRISPR/Cas9 system for plant genome editing and beyond
... the CRISPR/Cas9 system in plants is provided in Table 1 –3 ...of CRISPR/Cas9, ZFNs and TALENs ZFNs and TALENs function as dimers and only protein components are ...the ...
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Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections.
... The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein–Barr virus, cytomegalovirus, and Kaposi’s ...
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In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing
... The CRISPR/Cas9 system facilitates site-specific cleavage of the chromosome from various ...the CRISPR/Cas9 system has been developed as a universal genome-editing tool and has ...
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Efficient Genome Editing in Apple Using a CRISPR/Cas9 system
... and CRISPR (clustered regularly interspaced short palindromic ...the CRISPR/Cas9 system is now utilized widely for editing the genome of various organisms of all types, including plants, ...
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A Multiplex CRISPR/Cas9 System for Use as an Anti-BmNPV Therapeutic
... Discussion CRISPR/Cas9-mediated gene editing technology has been widely used in insect pest control, sex regulation, silk protein synthesis, and infectious disease research ...multiplex ...
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Candida albicans Gene Deletion with a Transient CRISPR-Cas9 System
... and CRISPR-associated gene 9 (CRISPR-Cas9) systems are used for a wide array of genome-editing applications in organisms ranging from fungi to plants and ani- ...a CRISPR-Cas9 ...
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Genome Editing of Babesia bovis Using the CRISPR/Cas9 System
... the CRISPR/Cas9 system does not require the insertion of a selection marker into the genome, thus allowing the possibility of targeting an unlimited number of genes in a single ...the ...
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Genetic Screens in Human Cells Using the CRISPR-Cas9 System
... Cas9 is dictated by a 20-base pair sequence at the 5′-end of the sgRNA, allowing for much greater ease of construction of knockout reagents. Mutant cells lines and mice bearing multiple modified alleles can be ...
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The CRISPR/Cas9 system inactivates latent HIV 1 proviral DNA
... by Cas9. In a separate study, the CRISPR/Cas9 system was exploited to successfully generate the CCR5Δ32 deletion in pluripotent stem cells with 33% efficiency, as compared to 14% efficiency ...
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Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 System
... the CRISPR/Cas9 system, to introduce site-specific DNA double-strand breaks in the Plasmodium yoelii genome that can be repaired through homologous ...this system, we achieved high ...
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Development of a CRISPR-Cas9 System for Efficient Genome Editing of Candida lusitaniae
... optimized CRISPR system for C. lusitaniae. A transient CRISPR-Cas9 system was developed for ...This system does not rely on stable integration of CRISPR components into ...
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Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus
... the CRISPR/Cas9 system bypasses the need for homologous recombination alto- gether, as the Cas9 nuclease is directed to its target through asso- ciation with a short ...this system in ...
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Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
... The CRISPR/Cas9 system, has been widely applied in both prokaryotes and eukaryotes, such as ...the system has been used in ...the CRISPR-Cpf1 system was recently reported; ...
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Applications of the CRISPR–Cas9 System in Cancer Therapy, an Emphasis on Three Clinical Trials
... Keywords: CRISPR-Cas9; genome editing; Cancer; tumor; Immunotherapy ABSTRACT The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 ...
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Moderate the MAOA-L Allele Expression with CRISPR/Cas9 System
... The MAOA-L allele activity can cause antisocial behavior in both healthy and 12.. unhealthy people.[r] ...
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CRISPR Cas9 system for plant genome editing within the European Union
... Abstract CRISPR (clustered regularly interspaced short palindromic repeat) Cas9 systems is a novel technique for genome ...using CRISPR Cas9 to induce site-specific mutations in Arabidopsis ...
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A redesigned CRISPR/Cas9 system for marker free genome editing in Plasmodium falciparum
... Moreover, the loading capacity for donor DNA is also unsatisfactory; indeed, current systems may not accept larger exogenous DNA fragments for knock-in. As de- scribed above, in the system developed by Wagner et ...
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