Cross-Species Amplification
Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae
... for cross-species amplification in four marine economic scallop species (Argopecten irradians, Chlamys farreri, Chlamys nobilis, and Mizuhopecten yessoensis) belonging to the family ...showed ...
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Development of Polymorphic Microsatellite Markers from AFLP Products in the Xinjiang Arctic Grayling (Thymallus arcticus grubei) and a Test of Cross-Species Amplification
... fish species: Red seam bream (Pagrus major), Lenok (Brachymystax lenok), Sea perch (Lateolabrax japonicus), Taimen (Hucho taimen), and Amur grayling (Thymallus grubii) were assessed for cross-species ...
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Isolation and characterization of microsatellite loci in the Persian sturgeon (Acipenser persicus, Borodine, 1897) and cross-species amplification in four commercial sturgeons from the Caspian Sea
... sturgeon species are presented in Figure 2. Detailed results of cross-species amplification efficiency of the SSR primer pairs developed for Persian sturgeon tested on four Caspian Sea ...
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Cross species amplification of 36 cyprinid microsatellite loci in Phoxinus phoxinus (L ) and Scardinius erythrophthalmus (L )
... one species should amplify in a second species is higher the more closely related the two species ...of cross-species amplification ...
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Development of ten polymorphic microsatellite loci for the sea snake Hydrophis elegans (Elapidae: Hydrophiinae) and cross-species amplification for fifteen marine hydrophiine species
... snake species, Aipysurus laevis, (Lukoschek et ...group species (Lukoschek 2008). The 39 Hydrophis group species are closely related (Lukoschek and Keogh 2006), so in order to obtain poly- morphic ...
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Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross species amplification for the willow grouse (L lagopus)
... For microsatellite analysis, PCR amplification was per- formed with forward primers labeled with FAM NED, PET or VIC fluorescent dyes. The reaction was made with 6µL of “Qiagen Taq PCR mastermix” (Qiagen, Cat. No. ...
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Cross species amplification of 41 microsatellites in European cyprinids: A tool for evolutionary, population genetics and hybridization studies
... toxostoma species whereas Hy8 were assigned toward the ...another species based on microsatel- lites), although most of them exhibited an intermediate morphology (Additional file ...
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Analysis of microsatellites in the vulnerable orchid Gastrodia flavilabella: the development of microsatellite markers, and cross-species amplification in Gastrodia
... Twenty individuals from each of four taxa in Gastrodia, including G. flavilabella from Nantou, G. elata from China, G. javanica (Blume) Lindl. from Lanyu Islet, and G. confusoides T. C. Hsu, S. W. Chung & C. M. Kuo ...
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Isolation and characterization of polymorphic microsatellite markers for the chub mackerel (Scomber japonicus) and cross-species amplification in the blue mackerel (S. australasicus).
... PCR amplification was performed in a 25-μL total volume containing ...94°C, amplification proceeded for 40 cycles (94°C for 30 s, 37°C for 40 s, 72°C for 45 s) and a final extension for 5 min at ...
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Isolation and characterization of microsatellite loci from two inbreeding bark beetle species (Coccotrypes)
... In a test panel of 29 unrelated C. carpophagus individuals and 41 unrelated individuals of C. dactyliperda, 13 of the 14 markers developed for C. carpophagus were polymorphic, as were 13 of the 14 markers developed for ...
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Biases underlying species detection using fluorescent amplified-fragment length polymorphisms yielded from roots
... tion, amplification, and quantification of amplified frag- ...and species, and these differences in underlying chemistry can affect DNA yields [11, ...herbaceous species [18], woody tree roots often ...
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Differentiation between Mycobacterium tuberculosis and Mycobacterium avium by Amplification of the 16S 23S Ribosomal DNA Spacer
... each species of other bacteria were dot blotted onto a nylon filter and were hybridized with the digoxigenin-labeled MT232 oligonucleotide ...other species of bac- teria, including all MAC ...
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Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex
... Differentiation of slowly growing Mycobacterium species including Mycobactenium tuberculosis, by gene amplification and restriction fragment length polymorphism analysis.. Identification[r] ...
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Species specific detection of Legionella pneumophila in water by DNA amplification and hybridization
... pneumophila by the polymerase chain reaction, followed by detection of the amplified product by hybridization of a radiolabeled oligodeoxynucleotide.. After 35 cycles of amplification, a[r] ...
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Alteration of the Chronic Wasting Disease Species Barrier by In Vitro Prion Amplification
... The sPMCA adaptation of CWD for the prairie vole M. ochrogaster presented in this report is qualitatively consistent with in vivo adaptation results we are observing in the meadow vole (M. pennsylvanicus), a closely ...
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Signal Integration in Quorum Sensing Enables Cross Species Induction of Virulence in Pectobacterium wasabiae
... pathogen species are often isolated from diseased tubers within the same affected crops (33) and the same potato tubers ...other species might be beneficial in both ...
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Genotyping of Candida albicans isolated from animals using 25S ribosomal DNA and ALT repeats polymorphism in repetitive sequence
... However, to the best of our knowledge, no comprehensive studies have been conducted on Candida species isolated from animals in Iran. Molecular diagnostic methods are available; however, there is limited ...
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<p>Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay to detect <em>Neisseria meningitidis</em></p>
... More recently, multiple cross displacement ampli fi - cation (MCDA) was developed to overcome the techni- cal barriers posed by current isothermal ampli fi cation strategies. MCDA assay was conducted under isother- ...
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Comparison of some Molecular Markers for Tick Species Identification
... tick species collected from the same location in the same sampling ...the species, mostly due to either bad storage of samples or loss of mouth-parts while removal the tick from the ...
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An epidemiological and serological study of Rickettsia in Western Australia
... 1.4 Figure showing the amplification and sequence similarities requirement of 5 rickettsial genes to determine group/ species/novelty of a Rickettsia isolate. 29[r] ...
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