cullin-RING-ubiquitin ligases
Cullin-RING ubiquitin ligases: global regulation and activation cycles
... Cullin-RING ubiquitin ligases (CRLs) comprise the largest known category of ubiquitin ...a cullin, RING H2 finger protein, a variable substrate-recognition subunit (SRS), ...
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Sequential Processivity and CAND1 Regulate SCF Ubiquitin Ligases
... E3 ligases are aptly named because they interact directly with the protein to be ...E3 ligases. HECT domain E3s accept ubiquitin from an E2 onto a catalytic cysteine, while RING (really ...
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The structure and regulation of Cullin 2 based E3 ubiquitin ligases and their biological functions
... CRLs: Cullin-RING E3 ubiquitin ligase complexes; Cul2: Cullin-2; Rbx1: RING protein; ECS: Elongin B and C-Cul2 or Cul5-SOCS box protein; VHL: von Hip- pel–Lindau; HIFα: α subunits of ...
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Mechanistic Dissection of the Cop9 Signalosome’s Deneddylation Activity on Cullin-RING Ligases
... initiates deactivation of a neddylated CRL complex by reversing the Nedd8 modification. The deconjugated CRL has several fold less ubiquitin ligase activity (Read, Brownell et al. 2000, Kawakami, Chiba et al. ...
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Rotavirus NSP1 Associates with Components of the Cullin RING Ligase Family of E3 Ubiquitin Ligases
... the ubiquitin-like protein ...deneddylated cullin to regulate the CRL complex. The presence of Nedd8 on a cullin blocks CAND1 binding, sug- gesting that CAND1 binds to a cullin only after ...
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Genetic and molecular characterization of Roc-Cullin interactions in Drosophila melanogaster E3 ubiquitin ligases
... 200 RING proteins that have been reported [15], yet very few of them have been studied in the context of ...The RING finger was first suggested to be involved in ubiquitylation by a study where Potuschak et ...
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Rotavirus NSP1 Requires Casein Kinase II Mediated Phosphorylation for Hijacking of Cullin RING Ligases
... N-terminal RING domain motif ...E3 ubiquitin ligase, triggers I B ubiquitination and proteasomal degradation, allowing nuclear translocation of the NF- B heterodimer ...
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Cullin-RING ligases in regulation of autophagy
... of cullin with one NEDD8 molecule removes the inhibitory binding to CAND1 and restores the CRLs in an active conformation ...of ubiquitin- loaded E2 to CRLs, facilitates the initial ubiquitin ...
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The role of cullin 5-containing ubiquitin ligases
... of Cul5 [20]. Furthermore, PKC-induced RAMEC prolif- eration is enhanced by Cul5(S730A) [20]. Cul5(S730A) expression in RAMECs increases the levels of phospho- rylated MAPK and the translocation of the transcrip- tion ...
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E3 ubiquitin ligases and their control of T cell autoreactivity
... E3 ligases that regulate T cell function. (a) Multi-subunit E3 ligases (Skp1-Cullin-F-box (SCF), Cullin-Elongin BC-SOCS/VHL (CBC), and U-box) are anchored by a Cullin scaffold protein ...
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Cul3-KLHL20 ubiquitin ligase: physiological functions, stress responses, and disease implications
... The study unveiling a critical role of KLHL20 in autophagy termination was initiated by the identifica- tion of ULK1 as an interacting protein of the kelch-repeat domain of KLHL20 [77]. Subsequent studies found that ...
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Role of HECT ubiquitin protein ligases in Arabidopsis thaliana
... Ubiquitination is a kind of posttranslational modifi cation of proteins in eukaryotes, and it plays an important role in the growth and development of organisms. The ubiquitination of proteins is a cascade enzymatic ...
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Following Ariadne's thread: a new perspective on RBR ubiquitin ligases
... E3 RING domains: complexes of UbcH7 and RING1s can be identified by pull-down and co-immunoprecipitation experiments ...RBR ligases recognize and bind substrates? Substrate binding has been reported for ...
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The KCTD family of proteins: structure, function, disease relevance
... via cullin binding, resulting in reduced Hh/ Gli signaling ...for ubiquitin- mediated protein ...1, cullin is fairly widely interaction with the family of KCTD ...
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Signaling-mediated control of ubiquitin ligases in endocytosis
... distal ubiquitin at the growing end of the polyubiquitin chain on the substrate allows enzyme Figure ...E3 ubiquitin ligases through recruitment to activated ...
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Regulation of SCF ubiquitin ligases by Jab1/Csn5 and the Cop9 signalosome
... This ubiquitin conjugation to substrate proteins occurs through the coordination of multiple ...First, ubiquitin is activated in a step that requires ATP hydrolysis and an ubiquitin-activating enzyme ...
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Heckmann, Irina (2016): DNA damage-induced degradation of elongating RNA polymerase II by a SUMO-dependent mechanism. Dissertation, LMU München: Fakultät für Biologie
... The transcription-coupled repair (TCR) factor Rad26 and its human homolog Cockayne syndrome (CS) protein B were previously described to act on stalled RNAPII. It is assumed that Rad26 and CSB are recruited to and travel ...
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Arabidopsis BRUTUS-LIKE E3 ligases negatively regulate iron uptake by targeting transcription factor FIT for recycling
... The E3 Ligase Domains of BTSL1 and BTSL2 Interact with FIT. To in- vestigate if FIT is a direct target of the BTSL E3 ubiquitin li- gases, we tested for protein interaction. Because BTSL protein levels are very ...
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The ubiquitin-proteasome system: A novel target for anticancer and anti-inflammatory drug research
... the ubiquitin-proteasome pathway involves several successive steps employing different classes of enzyme, namely ubiquitin-activating enzyme (E1), the ubiquitin-conjugating enzymes (E2s), the ...
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Multiple Interactions between the ESCRT Machinery and Arrestin-Related Proteins: Implications for PPXY-Dependent Budding
... served that mCherry-ARRDC1 could be recruited to the plasma membrane by the wild type but not by Gag lacking its PPXY motif (see supplemental Fig. 3 at http://www.kcl.ac.uk /content/1/c6/08/27/36/SupplJVRauch.pdf). ...
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