culture independent
Culture Based and Culture Independent Bacteriologic Analysis of Cystic Fibrosis Respiratory Specimens
... used culture and culture-independent methods to demonstrate the presence and diversity of anaerobic species in CF airways (17, ...used culture- based analyses to show that anaerobic bacteria ...
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A culture independent approach to unravel uncultured bacteria and functional genes in a complex microbial community
... Bacteria account for approximately half of the total carbon of the global biomass [1] and play fundamental roles in bio- geochemical cycles (e.g. C and N) [2]. Bacteria provide a free service worth trillions of dollars ...
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Analysis of Culture Dependent versus Culture Independent Techniques for Identification of Bacteria in Clinically Obtained Bronchoalveolar Lavage Fluid
... among culture-neg- ative BAL fluid ...in culture-negative ...a culture-independent approach but not via culture of the same specimen ...of culture growth, rather than indicating ...
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A Study of the Microbial Community at the Interface between Granite Bedrock and Soil Using a Culture Independent and Culture Dependent Approach
... both culture-independent and culture-dependent approaches were used to study the bacterial com- munity at the interface between granite bedrock and nutrient limiting soil in Dartmoor National Park, ...
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Clinical Microbiology Laboratories' Adoption of Culture Independent Diagnostic Tests Is a Threat to Foodborne Disease Surveillance in the United States
... stool culture interroga- tion when the laboratory knows what the offending pathogen is that needs to be targeted (this would also reduce the associated ...for culture, and likewise for Campylobacter ...for ...
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Analysis of microbial community in Japanese vineyard soils by culture-independent molecular approach
... a culture-independent molecular ...nine independent Japanese vineyards, and the results suggested that Ascomycota is the dominant group in the fungal community, whereas Proteobacterium and ...
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Culture Independent Molecular Analysis of Microbial Constituents of the Healthy Human Outer Ear
... Recently, culture-independent molecular methods of micro- bial identification and characterization have been developed and applied in the context of microbial ...
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Comparison of Standard Culture Based Method to Culture Independent Method for Evaluation of Hygiene Effects on the Hand Microbiome
... standard culture-based methods, but standard test methods for culture-independent microbiome characterization are ...among culture plate counts, 16S rRNA gene sequencing of DNA extracted ...
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Culture Independent Identification of Periodontitis Associated Porphyromonas and Tannerella Populations by Targeted Molecular Analysis
... Targeted culture-independent molecular ecology studies have a valuable role to play in the identification of bacterial targets for further investigations of the pathogenesis of bacterial ...
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A culture-independent approach to unravel uncultured bacteria and functional genes in a complex microbial community
... Most microorganisms in nature are uncultured with unknown functionality. Sequence-based metagenomics alone answers ‘who/what are there?’ but not ‘what are they doing and who is doing it and how?’. Function-based ...
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Characterization of the microbiota in the guts of Triatoma brasiliensis and Triatoma pseudomaculata infected by Trypanosoma cruzi in natural conditions using culture independent methods
... using culture independent methods based on the amplification of the 16S rRNA gene by polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), DNA fragment cloning, Sanger sequencing ...
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Culture Independent Genome Sequencing of Clinical Samples Reveals an Unexpected Heterogeneity of Infections by Chlamydia pecorum
... a culture-independent DNA capture approach to sequence ...was independent of the anatomical site sampled and the host ...strains. Culture-independent probe- based genome capture and ...
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Culture Independent Identification of Pathogenic Bacteria and Polymicrobial Infections in the Genitourinary Tract of Renal Transplant Recipients
... a culture-independent 16S rRNA-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract ...for culture-positive ...
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Bacterial water quality and network hydraulic characteristics: a field study of a small, looped water distribution system using culture-independent molecular methods
... Sekar, R., Deines, P., Machell, J. et al. (3 more authors) (2012) Bacterial water quality and network hydraulic characteristics: a field study of a small, looped water distribution system using ...
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Culture dependent and culture independent methods reveal diverse methylotrophic communities in terrestrial environments
... However, culture-independent approaches to studying methylotrophic microbial populations have suggested that the diversity of methylotrophs in the environment has not been exhaustively sampled and brought ...
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A Culture Independent and Culture Dependent Study of the Bacterial Community from the Bedrock Soil Interface
... a culture-independent and culture-dependent approach was used to study the bacterial community at the interface between basaltic bedrock and nutrient limiting soil in Cadiar Idris region of Snowdonia ...
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Culture-Independent Metagenomic Surveillance of Commercially Available Probiotics with High-Throughput Next-Generation Sequencing
... mock culture mixture was not as accurate as the mock DNA mixture, which was extremely accurate for strains with abundances of ⬎ 3% (Table ...mock culture mixture is less accurate quantitatively, the ...
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Use of a culture-independent approach to characterize aerosolized bacteria near an open-freestall dairy operation
... the results should be used cautiously, as the variable nature of aerosol emissions may not have been accounted for during this study. In addition, the specificity of the universal primer sets among a broad range of ...
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Mapping axillary microbiota responsible for body odours using a culture-independent approach
... previous culture-based assessments of the axillary bacteria-odour relation, which showed that certain members of the genus Staphylococcus were involved in the generation of odours ...a culture-based study ...
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Culture Dependent and Independent Investigations of Microbial Diversity on Urinary Catheters
... to culture. In this study we used culture-based and 16S rRNA gene-based culture-indepen- dent methods (fingerprinting, cloning, and pyrosequencing) to determine the microbial diversity of biofilms on ...
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