Cumulus Cells
Detection of RUNX2 gene expression in cumulus cells in women undergoing controlled ovarian stimulation
... the cumulus matrix, thus promoting a suc- cessful ovulation ...in cumulus cells with a lower number of follicles and retrieved ...lus cells matrix is expanded and then declines acting as an ...
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Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs
... granulosa cells, the EGF-like factor produced in granulosa cells by LH surge acts on cumulus cells to induce cumulus ...granulosa cells to cumulus ...granulosa ...
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Hyaluronan fragments generated by sperm secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization
... as cumulus cells express primarily HAS2 (Fülöp et ...on cumulus cells, ovulated COCs were treated with exogenous hyaluronidase or were cultured with sperm that are known to secrete ...in ...
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MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation
... confluent cumulus cells were co-transfected with 800 ng/ml of pmirGLO Dual-Luciferase miRNA target reporter vector harboring of the 3′ UTR of the target genes or a mismatch of the target gene and 50 ...
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The Effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes
... Cumulus cells have been considered to play an important role in oocyte maturation by keeping the oocyte under meiotic arrest, inducing meiotic resumption and by supporting cytoplasmic ...and cumulus ...
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Oocyte derived BMP15 and FGFs cooperate to promote glycolysis in cumulus cells
... in cumulus cells, an inhibitor of FGF receptor-dependent protein kinase, SU5402 (Mohammadi et ...OOX cumulus cells co-cultured with oocytes ...OOX cumulus cells was decreased in ...
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Effect of Cumulus Cells on the Efficiency of Vitrified Thawed Immature Cattle Oocytes
... with cumulus cells and without cumulus cells were exposed to the vitrification solution in a two-step manner (which was used to improve the cleavage rate and the developmental competence of ...
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Tribbles expression in cumulus cells is related to oocyte maturation and fatty acid metabolism
... ovarian cells is still ...follicular cells surrounding the oocytes before and after meiotic maturation in dif- ferent species has reported TRIB1 among the genes upregulated in mature follicles of three ...
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Altered microRNAs expression profiling in cumulus cells from patients with polycystic ovary syndrome
... The cumulus cells are a subset of granulose cells which maintain an intimate connection with the oocyte and are responsible for providing several tropic and metabolic factors to the pre-ovulatory ...
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Easy and rapid method for the determination of gene expression in cumulus cells incubated for oocyte maturation
... of cells. The cumulus cells collected from COCs before maturation were lysed in the cell lysis buffer provided in the Cells-to-cDNA II ...the Cells-to-cDNA II kit or RNase-free DNase ...
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Anti Müllerian Hormone (AMH) level and expression in mural and cumulus cells in relation to age
... Granulosa cells and FF for protein level were obtained from the same ...follicles. Cumulus GCs were obtained during oo- cyte denudation for ...the cumulus cells were removed from the ...
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Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI
... A R conceived the study, contributed to the final interpretation of the data, the drafting of the manuscript and gave his final approval. S C was involved in: experimental design, oocyte collection and culture, oocyte ...
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Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: BMP15 and GDF9 control cholesterol biosynthesis in cumulus cells
... fragile and unstable (Yan et al., 2001) indicating that GDF9 and BMP15 are essential for the normal development of cumulus-oocyte complexes (COCs). Although in-vitro studies using recombinant GDF9 and BMP15 ...
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RUNX2, GPX3 and PTX3 gene expression profiling in cumulus cells are reflective oocyte/embryo competence and potentially reliable predictors of embryo developmental competence in PCOS patients
... the expression levels of four genes (CXCL1, CXCL2, CXCL3, ITGB5) were decreased. To our knowledge, these four genes, which are related with the inflammatory re- sponse (CXCL1, CXCL2, CXCL3) or cell–matrix adhesion ...
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Semi-quantitative measurement of specific proteins in human cumulus cells using reverse phase protein array
... somatic cells (cumulus cells [CCs] and/or granulosa cells [GCs]) surrounding the oocyte since their interactions are in- volved in the acquisition of oocyte meiotic and develop- mental ...
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Is there a correlation between follicle size and gene expression in cumulus cells and is gene expression an indicator of embryo development?
... promoting cumulus expansion and oocyte competence ...facilitate cumulus expansion through induction of cumulus matrix genes [5] and are important in the survival of mouse cumulus cells ...
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IDENTIFICATION OF HYALURIC ACID SYNTHESIS 2 (HAS2) AND GREMLIN 1 (GREM1) GENE EXPRESSIONS IN HUMAN CUMULUS CELLS AS A BIOMARKER FOR OOCYTE QUALITY
... the cumulus cell plays important role in signaling for follicular development as well as for oocyte ...the cumulus cells that can become a useful marker for oocyte ...on cumulus cells ...
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Cumulus cells steroidogenesis is influenced by the degree of oocyte maturation
... affect cumulus cell steroidogenesis through the production of soluble ...cine cumulus cells were maintained in culture conditions that steadily produced steroidal hormones in four days, yielding ...
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Correlation of cumulus gene expression of GJA1, PRSS35, PTX3, and SERPINE2 with oocyte maturation, fertilization, and embryo development
... The cumulus–oocyte complexes (COCs) in antral folli- cles with a diameter larger than 14 mm were collected using transvaginal ultrasound and a 16-gauge needle and were exposed to 80 IU hyaluronidase in Quinn’s ...
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The art of oocyte meiotic arrest regulation
... rather not the oocyte. In contrast, study in cow demon- strated that none of the NPP family member (NPPA, NPPB or NPPC), individually or in combination, inhibited the rate of meiosis resumption of COC in vitro, despite ...
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