Cupriavidus necator
Efficient biochemical production of acetoin from carbon dioxide using Cupriavidus necator H16
... Background: Cupriavidus necator is the best-studied knallgas (also termed hydrogen oxidizing) bacterium and pro- vides a model organism for studying the production of the storage polymer polyhydroxybutyrate ...
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β-Ketothiolase Homologues in Cupriavidus Necator
... enzymes. Cupriavidus necator (formerly known as Ralstonia eutropha) was found to be capable of producing the P(3HB) homopolymer from even carbon numbered n- alkanoates while odd-carbon numbered n- ...
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An engineered constitutive promoter set with broad activity range for Cupriavidus necator H16
... ABSTRACT: Well-characterized promoters with variable strength form the foundation of heterologous pathway optimization. It is also a key element that bolsters the success of microbial engineering and facilitates the ...
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Global changes in the proteome of Cupriavidus necator H16 during poly-(3-hydroxybutyrate) synthesis from various biodiesel by-product substrates
... by Cupriavidus necator H16 in batch cultures was evaluated using three biodiesel-derived by-products as the sole carbon sources: waste glycerol (REG-80, refined to 80 % purity with negligi- ble free fatty ...
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13C assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16
... Cupriavidus necator H16 is a facultative anaerobe, which can switch to anaerobic respiration by using NO 3 and NO 2 as electron acceptors. It grows utilizing a variety of organic substrates ranging from ...
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Evaluation of gene expression cassettes and production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a fine modulated monomer composition by using it in Cupriavidus necator
... 5. Cupriavidus necator H16 has two phaJ genes, which encode enzymes capable of efficiently catalyzing the conversion from β-oxidation intermediate (2-hexenoyl- CoA) to a precursor of the 3HHx monomer unit ...
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Use of agro-industrial residue from the canned pineapple industry for polyhydroxybutyrate production by Cupriavidus necator strain A-04
... Lignocellulosic biomass has become an attractive alter- native to fossil resources for the production of biofuels and various biochemical reagents, including biodegrad- able PHAs, which are the most abundant in nature. ...
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An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture
... Genome-wide transcriptional analysis was performed to investigate genes that are differentially expressed in re- sponse to derepression of hydrogenase genes expression. For this purpose, we analysed transcripts of two ...
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The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16
... C. necator for its tolerance towards 3‑HP, it was noted that it could utilise the compound as the sole source of carbon and energy, a highly undesirable trait in the context of biological 3‑HP production which ...
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Characterisation of a 3 hydroxypropionic acid inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator
... C. necator H16 (Supplementary Table ...C. necator, however, the arrangement of genes is ...C. necator hpdH and its putative LTTR (here termed ...C. necator acaD-mmsA2-mmsB ...
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Production of polyhydroxyalkanoates from waste frying oil by Cupriavidus necator
... The maximum production of PHB from waste frying oil (1.2 g/l) was achieved after 72 hours of fermenta- tion in TSB. The PHB production results in this study were low in comparison to fermentations with Cupria- vidus ...
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High-quality permanent draft genome sequence of the Parapiptadenia rigida-nodulating Cupriavidus sp. strain UYPR2.512
... to Cupriavidus necator ATCC 43291 T , Cupriavidus oxala- ticus DSM 1105 T and Cupriavidus taiwanensis LMG 19424 T based on the 16S rRNA gene alignment with se- quence identities of ...[11]. ...
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A transcription factor based biosensor for detection of itaconic acid
... reporter gene expression in response to itaconate was measured by fluorescence output in the.. coli MG1655 and the betaproteobacterium Cupriavidus necator.[r] ...
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Massively parallel sequencing and analysis of the Necator americanus transcriptome
... Found at: doi:10.1371/journal.pntd.0000684.s003 0.04 MB XLS Table S4 Description of Caenorhabditis elegans orthologues of Necator americanus contigs for which inferred peptides were asso[r] ...
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Identification of Two Different 14 α Sterol Demethylase Related Genes (cyp51A and cyp51B) in Aspergillus fumigatus and Other Aspergillus species
... albicans due to single point mutations which seem to have a cooperative effect (20, 24, 32). On the other hand, single point mutations have been shown to be directly related to fungal DMI resistance in filamentous fungi. ...
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Reinfection with hookworm after chemotherapy in Papua New Guinea
... Reinfection with hookworm Necator americanus following chemotherapy was studied over 2 years in a rural village in Madang Province, Papua New Guinea.. The prevalence of hookworm infecti[r] ...
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Of dogs and hookworms: man’s best friend and his parasites as a model for translational biomedical research
... Comparison of mitochondrial cytochrome oxidase 1 DNA sequences from Necator americanus hookworms maintained for 100 generations in golden hamsters Mesocricetus auratus and hookworms from[r] ...
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Human dendritic cell sequestration onto the Necator americanus larval sheath during ex sheathing: a possible mechanism for immune privilege
... DCs sequestration around the sheath and unchallenged migration of larvae could explain the inefficiency of immune responses against Necator americanus.. Despite its profound health impli[r] ...
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Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator
... FDA is lipophilic, membrane-permeable, non-fluorescent, and it is hydrolyzed to polar fluorescent molecules in the cytoplasm of living cells. These molecules remain in the cytoplasm of living ascospores with intact cell ...
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Effects of Soil Application and Cultivation Methods on the Development of Powdery Mildew (Erysiphe necator Schwein ) and Yield in Sultanina Grape
... Powdery mildew attacks could not reach to an epidemic level in the vineyards where the research was carried out. This situation can be explained that climatic conditions and especially high temperatures could directly ...
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