Cycle Threshold.
Clostridium difficile PCR Cycle Threshold Predicts Free Toxin
... PCR cycle threshold in PCR-positive toxin-positive and toxin-negative stool samples using various toxin reference ...median cycle thresholds. Cycle threshold cutoffs— derived based on ...
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Toxin B PCR Amplification Cycle Threshold Adds Little to Clinical Variables for Predicting Outcomes in Clostridium difficile Infection: a Retrospective Cohort Study
... say (EIA) or nucleic acid amplification test (NAAT), and samples with a positive screen- ing test should subsequently be retested with a toxin A/B EIA to identify patients infected with a toxigenic strain, who have the ...
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Improved performance of Xpert MTB/RIF assay on sputum sediment samples obtained from presumptive pulmonary tuberculosis cases at Kibong’oto infectious diseases hospital in Tanzania
... The raw sputum was tested as recommended by the manufacturer of Xpert, here referred to as standard dilu- tion. Unprocessed raw sputum was diluted with sample reagent (SR) at a ratio of 1:2 [15]. Sputum sediments were ...
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Notch pathway connections in primary leukaemia samples of limited size
... second set, the template was derived by direct reverse transcription of RNA in cell lysates and subsequent 15 cy- cles amplification of multiplex PCR reaction (PCRI). The gene expression level is presented as a Delta Ct ...
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Evaluation of fluorimetry and direct visualization to interpret results of a loop mediated isothermal amplification kit to detect Leishmania DNA
... Ct: cycle threshold; FIND: Foundation for Innovative New Diagnostics; IFAT: immunofluorescence antibody test; kDNA: kinetoplast deoxyribonucleic acid; LAMP: loop-mediated isothermal amplification; LED: ...
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Circulation of the low pathogenic avian influenza subtype H5N2 virus in ducks at a live bird market in Ibadan, Nigeria
... Molecular detection, virus isolation, and subtyping Twenty (13%) out of the 155 randomly collected cloaca swab samples in ducks from the LBM (see Additional file 2) were positive for avian influenza virus subtype H5 by ...
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Targeting the local tumor microenvironment with vaccinia virus expressing B7 1 for the treatment of melanoma
... This suggests that local activation of tumorNonstandard abbreviations used: aRNA, amplified RNA; CEA, carcinoembryonic antigen; CT, cycle threshold; CTLA-4, CTL-associated antigen 4; ECO[r] ...
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Olszewski, Konrad (2018): Differentielle Genexpressionsanalysen aus Muskeleinzelfasern bei mitochondrialen Myopathien. Dissertation, LMU München: Medizinische Fakultät
... Die Anzahl an Vervielfältigungszyklen bis zum erstmaligen exponentiellen Anstieg des Fluoreszenzsignals (der sog. Ct-Wert, cycle threshold) kann mit den Ct-Werten ande[r] ...
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SPECIAL COMMUNICATION Evaluation of correlation between expression of P53 and Malondialdehyde levels in prostate cancer patients
... The analytical study was conducted at the National University of Sciences and Technology, Islamabad, Pakistan from Nov 2012 to Nov 2013 to find out, correlate and assess negative correlation of serum malondialdehyde ...
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Mechanical Disruption of Lysis Resistant Bacterial Cells by Use of a Miniature, Low Power, Disposable Device
... We evaluated the capability of the OmniLyse device to me- chanically disrupt thick-walled organisms and liberate DNA suitable for PCR amplification, relative to the BioSpec Mini- BeadBeater and Mini BeadBeater-8, two ...
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Evaluation of two DNA extraction methods for the PCR based detection of eukaryotic enteric pathogens in fecal samples
... PCR cycle threshold (Ct) values, the efficiency of two DNA extraction kits: the semi-automated EZ1 ® (Qiagen) and the manual QIAamp ® DNA Stool Mini Kit (Qiagen), on six protozoa: Blastocystis ...
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Endogenous vascular synthesis of B type and C type natriuretic peptides in the rainbow trout
... anterior cardinal vein atrial natriuretic peptide B-type natriuretic peptide celiacomesenteric artery C-type natriuretic peptide cycle threshold diaminodipropyl-amine efferent branchial [r] ...
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HER2 and ESR1 mRNA expression levels and response to neoadjuvant trastuzumab plus chemotherapy in patients with primary breast cancer
... Ct: cycle threshold; ER: estrogen receptor; ESR1: estrogen receptor 1; FDA: Food and Drug Administration; FFPE: formalin-fixed paraffin-embedded; GOI: genes of interest; HER2: human epidermal growth factor ...
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A quantitative PCR method for measuring absolute telomere length
... Telomere lengths, in different cell types, showed good agreement between the reported TRF values and the measures we obtained by the absolute qPCR method (Figure 2). However, there is a consistent discrepancy between the ...
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The p53 response in single cells is linearly correlated to the number of DNA breaks without a distinct threshold
... Activated ATM subsequently phosphorylates and sta- bilizes p53, which shows a series of highly regulated, un- damped pulses in single cells upon induction of DSBs. The amplitude and duration of p53 pulses is independ- ...
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A novel methyl-binding domain protein enrichment method for identifying genome-wide tissue-specific DNA methylation from nanogram DNA samples
... QPCR was performed for the known brain/retina differ- entially methylated genes Rho (Gene ID 212541) and Rbp3 (19661) [19] and a known equally methylated gene, H19 (14955) [20]. QPCR was performed on an iQ™5 in- strument ...
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Degradome expression profiling in human articular cartilage
... Fold change and median threshold cycle for all serine and threonine protease genes showing significant difference at P < 0.01 between osteoarthritis OA versus fracture to the neck of fem[r] ...
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Secretome analysis of chondroitin sulfate treated chondrocytes reveals anti angiogenic, anti inflammatory and anti catabolic properties
... Abbreviations CS: chondroitin sulfate; Ct: threshold cycle; DMEM: Dulbecco’s modified Eagle’s medium; ECM: extracellular matrix; GS: glucosamine sulfate; HAC: human articular chondrocyte[r] ...
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ETS1 variants confer susceptibility to ankylosing spondylitis in Han Chinese
... parative threshold cycle (Ct) method and normalized to that of human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), calculated by the 2 -△△Ct method and log 10 ...
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EXPRESSION OF GLUCOSAMINE 6 PHOSPHATE ISOMERASE AND JACOB GLYCOPROTEIN DURING CULTURE OF ISOLATES OF ENTAMOEBA HISTOLYTICA
... INTRODUCTION: E. histolytica is an intestinal protozoan parasite and the causative agent of amebic dysentery and liver abscess in humans. This parasite remains a significant cause of morbidity and mortality in developing ...
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