Cyclin-dependent kinase 1
Mitotic p21 Cip1/CDKN1A is regulated by cyclin-dependent kinase 1 phosphorylation
... We showed previously that p21 deficiency causes segregation defects which could be rescued by inhibiting Cdk1 activity with the Cdk1 inhibitor RO-3306 or by adding back wild type p21 [6]. Interfering with the ...
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Cyclin-Dependent Kinase 1/Cyclin B1 Phosphorylates Varicella-Zoster Virus IE62 and Is Incorporated into Virions
... a 1:40 dilution of rPOka/HFFs for 2 ...a 1:50 dilution of rPOka/HFFs, and washed with fresh medium 2 h ...for cyclin B1, IE62, and TGN46 (Serotec) as described pre- viously ...
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Inhibition of Cell Division by the Human Cytomegalovirus IE86 Protein: Role of the p53 Pathway or Cyclin-Dependent Kinase 1/Cyclin B1
... Since p21 can be induced by a p53-independent mechanism (19, 22), we determined whether the IE86 protein induces endogenous p21 in the absence of functional p53. U373MG cells, human glioblastoma-astrocytoma cells that ...
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<p>New Isatin–Indole Conjugates: Synthesis, Characterization, and a Plausible Mechanism of Their in vitro Antiproliferative Activity</p>
... gene 1, cell cycle-associated proteins (cyclin B1, cyclin D1, and phos- phorylated cyclin-dependent kinase 1), and a pro-apoptotic protein (Bcl-2-associated X protein ...
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Identification of core genes in ovarian cancer by an integrative meta-analysis
... benzimidazoles 1; CAMs: Cell adhesion molecules; CCNB2: G2/mitotic-specific cyclin-B2; CDC20: Cell-division cycle protein 2; CDK1: Cyclin-dependent kinase 1; CENPA: Centromere ...
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Downregulation of cyclin dependent kinase 2 activity and cyclin A promoter activity in vascular smooth muscle cells by p27(KIP1), an inhibitor of neointima formation in the rat carotid artery
... cdk2 kinase activity by p27 in primary cultures of ...Fig. 1 A , cdk2 activity in primary cultures of VSMCs was markedly downreg- ulated in serum-deprived cells ( Q , quiescent), as compared to ...
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USP4 function and multifaceted roles in cancer: a possible and potential therapeutic target
... Protein kinase B; ARF-BP1: ARF-binding protein 1; BRAC : Breast invasive carcinoma; CARD: Caspase recruitment domain; CDK: Cyclin-dependent kinase; CypA: Cyclophilin A; DNPEP: PAK5- ...
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Cyclin-dependent kinase 2 is an ideal target for ovary tumors with elevated cyclin E1 expression
... Figure 1: The status of CCNE1 overexpression is not associated with tumorigenic behaviors in established ovarian cancer cells. A.Total RNA was isolated from overnight-cultured cells and subjected to qRT-PCR to ...
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Regulation of Ciz1 by Cyclin A Cyclin Dependent Kinase 2 (CDK2) mediated phosphorylation
... Cells were trypsinised as described (section 2.2). Following trypsinisation, cells were centrifuged at 500g for minutes to form a pellet, which was then frozen at -80 o C. RNA extraction was performed using the PureLink ...
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MicroRNAs in systemic rheumatic diseases
... CDK2, cyclin-dependent kinase 2; EGFL7, epidermal growth factor-like domain 7; IFN, interferon; IL, interleukin; IRAK1, IL-1 receptor- associated kinase 1; MCP-1, monocyte ...
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Landscape of genomic alterations in high-grade serous ovarian cancer from exceptional long- and short-term survivors
... CCNE1: Cyclin E 1; CD4: Cluster of differentiation 4; CD8: Cluster of differentiation 8; CDK12: Cyclin dependent kinase 12; CNV: Copy number variation; DLEU1: Deleted in lymphocytic ...
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Regulation of lipogenesis by cyclin dependent kinase 8–mediated control of SREBP 1
... The nuclear/active form of SREBP-1 is processed from its pre- cursor initially located in ER membrane (13). Since CDK8 is pre- dominantly a nuclear protein (33), it is less likely that CDK8 can directly regulate ...
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Cyclin dependent kinase regulation in haemopoietic cells
... For these reasons, a definitive study is required to determine the abundance of cdks in different cell cycle phases of proliferating cells. Ideally, this study should be performed using primary cells that are 100% ...
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Proteomic analysis of differentially expressed proteins in hepatitis B virus-related hepatocellular carcinoma tissues
... protein kinase 1 and Protein ...named Cyclin- dependent kinase inhibitor p12, Cyclin-dependent kinase inhibitor 1, Antioxidant protein 2, Protein disulfide ...
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Retroviral Cyclin Controls Cyclin-Dependent Kinase 8-Mediated Transcription Elongation and Reinitiation
... Figure 1 shows the normalized fold changes in tran- script levels of FOS, EGR1, and cJUN in control and RV-cyclin- expressing cells at each time ...RV- cyclin expression at its peak, ...(Fig. ...
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Modulation of apoptosis by the cyclin dependent kinase inhibitor p27Kip1
... permits cyclin-dependent kinase 2 (CDK2) activity to be generated, allowing the cell to enter the S phase, followed by completion of the cell cycle ...
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Inhibition of Human Immunodeficiency Virus Type 1 Transcription by Chemical Cyclin-Dependent Kinase Inhibitors
... of cyclin E-cdk2 complex, in- creased retinoblastoma protein (Rb) phosphorylation, in- creased HIV-1 transcription, and viral progeny formation ...other cyclin-cdk complexes or many other cellular ...
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Regulation of the G1-S transition by cyclin-dependent kinase inhibitors
... Ink4a dependent (86). In addition, loss of Bmi-1, a known repressor of p16 Ink4a (48) significantly impairs the self- renewal ability of NSCs, and this effect can be rescued by p16 Ink4a loss ...
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The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy
... RNA interference. Drosophila cells were transfected with double-stranded RNA as previously described in reference 20. dsRNA sequences used were derived with T7-binding sequence tagged primers, followed by in vitro ...
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Cyclin dependent kinase inhibitor 2B regulates efferocytosis and atherosclerosis
... Future studies are needed to determine whether efferocytosis can be induced as a means to slow plaque growth or potentially stimulate regression of the necrotic core. The fact that the simple application of exogenous ...
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