Cyclin-dependent kinase Inhibitor p21
Differential targeting of the cyclin-dependent kinase inhibitor, p21 CIP1/WAF1 , by chelators with anti-proliferative activity in a range of tumor cell-types
... the cyclin-dependent kinase inhibitor, ...targeting p21. All the chelators up-regulated p21 mRNA in the five tumor cell-types ...total p21 protein levels, these agents ...
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Adenovirus mediated over expression of the cyclin/cyclin dependent kinase inhibitor, p21 inhibits vascular smooth muscle cell proliferation and neointima formation in the rat carotid artery model of balloon angioplasty
... The p21 protein is a negative regulator of mammalian cell cycle progression that functions both by inhibiting cyclin dependent kinases (CDKs) required for the initiation of S phase, and by binding to ...
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Loss of G1/S Checkpoint in Human Immunodeficiency Virus Type 1-Infected Cells Is Associated with a Lack of Cyclin-Dependent Kinase Inhibitor p21/Waf1
... lysed with 1 ml of lysis buffer containing 50 mM Tris-Cl (pH 7.4), 120 mM NaCl, 5 mM EDTA, 0.5% NP-40, 50 mM NaF (phosphotyrosine phosphatase inhibi- tor), 1 mM DTT, and 1 mM PMSF. The cells were incubated on ice for 15 ...
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Indirect p53-dependent transcriptional repression of Survivin CDC25C , and PLK1 genes requires the cyclin-dependent kinase inhibitor p21/CDKN1A and CDE/CHR promoter sites binding the DREAM complex
... Regulation of Survivin, CDC25C, and PLK1 by p53 was tested in HCT116 wild-type cells upon stimulation with the DNA damaging agent doxorubicin, the MDM2 inhibitor Nutlin-3a, or the pyrimidine analogue ...
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MicroRNA miR-155 Inhibits Bone Morphogenetic Protein (BMP) Signaling and BMP-Mediated Epstein-Barr Virus Reactivation
... MicroRNA miR-155 is expressed at elevated levels in human cancers including cancers of the lung, breast, colon, and a subset of lymphoid malignancies. In B cells, miR-155 is induced by the oncogenic latency gene ...
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Tumor suppression and inhibition of aneuploid cell accumulation in human brain tumor cells by ectopic overexpression of the cyclin dependent kinase inhibitor p27KIP1
... We demonstrated previously that Ad b Gal exhibits 100% Figure 1. Western blot analysis. Total cell lysates were prepared from U-373MG cells (0) and those infected for 72 h with AdbGal (Gal), a replication-defective ...
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Mitotic p21 Cip1/CDKN1A is regulated by cyclin-dependent kinase 1 phosphorylation
... of cyclin B1 and GST proteins were taken as positive and negative control, ...of p21 from different species and the S130 residue is highlighted in ...against p21-pS130. (D) GST-p21 and its ...
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The relationship between transcription initiation RNAs and CCCTC-binding factor (CTCF) localization
... the p21 (cyclin-dependent kinase inhibitor 1A gene, also known as CDKN1A) cp-tiRNA and STARD13 tiRNA sponges on nucleosomal positioning based on histone H3 ...
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Penta O galloyl β D glucose induces G1arrest and DNA replicative S phase arrest independently of P21 cyclin dependent kinase inhibitor 1A, P27 cyclin dependent kinase inhibitor 1B and P53 in human breast cancer cells and is orally active against triple negative xenograft growth
... protein kinase), a well-known autophagy signaling kinase in response to nutrient deprivation [18]; and by increased phosphatidylethanolamine modification of the microtu- bule-associated protein 1 light ...
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Enhanced susceptibility of cyclin kinase inhibitor p21 knockout mice to high fat diet induced atherosclerosis
... that p21 overexpression reduces mitogen- induced lymphocyte proliferation and inflammation, one of the key mediators in the pathogenesis of atherosclero- ...of p21 as a protective agent in ...in p21 ...
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p21 is decreased in polycystic kidney disease and leads to increased epithelial cell cycle progression: roscovitine augments p21 levels
... The cyclin-dependent kinase inhibitors (CKI) function in mitogenic signaling through their interaction with the cyclins and the cyclin dependent kinases [3], and through proliferating ...
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Drosophila Cdi4 is a p21/p27/p57-like cyclin-dependent kinase inhibitor with specificity for cyclin E complexes.
... Most bait plasmids are derived from pLEX(202+PL) (Ruden et al., 1991) or pEG202 (Estojak et al., 1995), which both contain the HIS3 gene, 2µm origin of replication, and the ADH1 promoter driving expression of fusion ...
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Motif co-regulation and co-operativity are common mechanisms in transcriptional, post-transcriptional and post-translational regulation
... of p21 gene, pre-mRNA and protein, showing known functional modules (see Table ...The p21 gene contains: two p53- responsive elements [159, 160]; four E-box motifs for binding Transcription factor AP-4 ...
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The cyclin dependent kinase inhibitor p57Kip2 mediates proliferative actions of PTHrP in chondrocytes
... include cyclin D1 and cyclin A (31, ...in cyclin D1 gene expression, or cyclin A protein levels, in PTHrP-null embryos (data not shown), so their role as mediators of PTHrP’s actions in ...
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Overexpression of p21waf1 in Human T-Cell Lymphotropic Virus Type 1-Infected Cells and Its Association with Cyclin A/cdk2
... the p21/waf1 is p53 independent and may involve other regulatory elements that are Tax responsive in the minimal ...for p21/waf1 activity (25). Identification of p21/waf1 partners in HTLV-1-infected ...
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Phosphorylation states change Otx2 activity for cell proliferation and patterning in the Xenopus embryo
... To determine whether known Otx2 activities are affected by phosphorylation states, we overexpressed phosphomimetic or nonphosphorylatable Otx2 in Xenopus embryos and analyzed the resulting eye phenotypes, which have been ...
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CDK9 inhibitors in acute myeloid leukemia
... There are ongoing efforts to determine predictive bio- markers to allow identification of specific subsets of pa- tients who are likely to respond to alvocidib, such as use of BH3 profiling [66]. As NOXA interacts most ...
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The role of cyclin D2 and p21/waf1 in human T cell leukemia virus type 1 infected cells
... the cyclin Ds and p21/waf1 are often seen deregulated in viral ...instance, cyclin D2 is upregulated in Epstein-Barr virus (EBV) infected cells [47,48] and cyclin D1 is upregulated in Sim- ian ...
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Retroviral Cyclin Enhances Cyclin-Dependent Kinase-8 Activity
... buffer. Master mixes containing all components were split for the inclu- sion of OA (1 to 100 nM) and dispensed to protein G beads. Reaction mixtures were mixed at setting 1 on a vortexer for 50 min at 30°C, sup- ...
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Geminivirus-Induced Gene Silencing of the Arabidopsis RETINOBLASTOMA-RELATED
... by cyclin-dependent kinases relieves E2F repression leading to activation of such as PCNA, CDC6, and RNR, which are necessary for DNA replication (Nevins, 1992; Ezhevsky et ...
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