cytometric assay
Development and Evaluation of a Tetraplex Flow Cytometric Assay for Quantitation of Serum Antibodies to Neisseria meningitidis Serogroups A, C, Y, and W-135
... microsphere assay would require approximately 3 ...the assay is enhanced even ...The assay could be expanded to include detection of antibodies to Streptococcus pneumoniae (16) and ...flow ...
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Perioperative variation in phagocytic activity against Candida albicans measured by a flow-cytometric assay in cardiovascular-surgery patients.
... Candidiasis is a well-known disease that occurs mainly in cancer patients and in those with other hematologic malignan- cies. Its incidence has been observed to be increasing in non- neutropenic surgical patients. In the ...
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Development of a multiparameter flow cytometric assay as a potential biomarker for homologous recombination deficiency in women with high grade serous ovarian cancer
... flow cytometric approach to measure DNA damage is rapid, more sensitive, and less cumbersome compared with alternative, commonly used methods, including the comet assay for single cell DNA damage assessment ...
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A Bead Based Flow Cytometric Assay for Monitoring Yersinia pestis Exposure in Wildlife
... flow cytometric assay developed for plague monitoring in wildlife called the F1 Luminex plague assay ...Additionally, assay formats that do not require species-specific immunological reagents, ...
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A flow cytometric assay to quantify invasion of red blood cells by rodent Plasmodium parasites in vivo
... In addition to a method for quantifying parasitaemia, a flow cytometric method for quantifying parasite invasion in vivo was developed. A major hurdle in this type of analysis is distinguishing parasite invasion ...
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Comparison of a Multiplex Flow Cytometric Assay with Enzyme-Linked Immunosorbent Assay for Quantitation of Antibodies to Tetanus, Diphtheria, and Haemophilus influenzae Type b
... multiplexed assay for anti-Hib, -Dip, and ...Luminex assay for IgG antibodies to HbO-HA and the Dip and Tet ...Luminex assay for quantitation of IgG antibodies to 14 PnPs sero- types, 1, 3, 4, 5, 6B, ...
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Use of Highly Encapsulated Streptococcus pneumoniae Strains in a Flow-Cytometric Assay for Assessment of the Phagocytic Capacity of Serotype-Specific Antibodies
... phagocytosis assay for Streptococcus pneumoniae based on flow cytometry (FACS) with human polymor- phonuclear cells and human complement was developed for the study of human vaccination ...immunosorbent ...
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Flow cytometric assay for quantifying opsonophagocytosis and killing of Staphylococcus aureus by peripheral blood leukocytes
... 3A, approximately 6% of the phagocytes displayed weak fluorescence at time zero, whereas 77.2% exhibited marked green fluorescence after 30 min of incubation with labeled staphylococci a[r] ...
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Multiple Strategies Reveal a Bidentate Interaction between the Nipah Virus Attachment and Fusion Glycoproteins
... Though a qualitative interaction assay is very useful for dem- onstrating membrane protein interactions, ideally the new flow- cytometric assay would be quantitative. Attempts with previously ...
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Association of CD69 Up-Regulation on CD4+ CLA+ T cells versus patch test, strip patch test and clinical history in nickel sensitization
... flow cytometric assay of cD 69 up- regulation (maximum AI) on the one hand and Pt, sPt, and clinical history on the other hand, we applied Roc analysis and the corresponding c statistic measur- ing the area ...
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Strong HIV-Specific CD4+ and CD8+ T-Lymphocyte Proliferative Responses in Healthy Individuals Immunized with an HIV-1 DNA Vaccine and Boosted with Recombinant Modified Vaccinia Virus Ankara Expressing HIV-1 Genes
... uptake assay and a flow-cytometric assay of specific cell-mediated immune response in activated whole blood (FASCIA-WB) 2 weeks after the HIV-MVA boost (n ⴝ ...uptake assay and CD4 ⴙ T-cell ...
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Identification of Shiga toxin binding sites in porcine tissues and to porcine leukocytes
... Reproducible results were obtained with all cell samples incubated in an estimated 750 ng/ml crude Stx2kw in the Stx2 binding flow cytometric assay.. No differences between pigs or betwe[r] ...
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A Flow Cytometric Opsonophagocytic Assay for Measurement of Functional Antibodies Elicited after Vaccination with the 23-Valent Pneumococcal Polysaccharide Vaccine
... flow cytometric assay were higher than those obtained by the manual ...flow cytometric assay, the transparent form would more likely be phagocytosed, leading to higher ...flow ...
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Temporal profiling of Kv1.3 channel expression in brain mononuclear phagocytes following ischemic stroke
... We have utilized a rapid flow cytometric assay of func- tional cell-surface Kv1.3 channels [13, 18, 21] to pheno- type acutely isolated CNS-MPs following tMCAO, and found that functional Kv1.3 channel ...
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Flow cytometric immunobead assay for quantitative detection of platelet autoantibodies in immune thrombocytopenia patients
... (SPRCA) assay [29], platelet immunofluorescence assay [30], anti- gen-capture assays such as the monoclonal antibody-spe- cific immobilization of platelet antigen assay (MAIPA) [11], and flow ...
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Abnormal CD40 Ligand (CD154) Expression in Human Immunodeficiency Virus-Infected Children
... flow cytometric assay originally developed in our laboratory as a diagnostic screening test for XHIM-1 was used to study CD154 expression on resting and activated peripheral blood T cells ...
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Original Article Downregulation of miR-155 inhibits proliferation and enhances chemosensitivity to Temozolomide in glioma cells
... blot assay (A); qRT-PCR assay (B); Cell apoptosis was detected by Flow cytometric analysis (C); Cell viability was detected by MTT assay (D); Bim cDNA or control ...blot assay (E); Cell ...
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Standardization and validation of a cytometric bead assay to assess antibodies to multiple Plasmodium falciparum recombinant antigens
... multiplex assay for antibody determination to ...on assay optimization condi- tions or comparisons of antigen and plasma concentra- tions for multiplex ...multiplex assay were determined and compared ...
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Flow cytometric immunofluorescence assay for detection of antibodies to human immunodeficiency virus type 1 using insoluble precursor forms of recombinant polyproteins as carriers and antigens
... Subjects. HIV-1 seroconversion panels obtained from BBI were used for the evaluation of r-FIFA. All of the panels were tested with several FDA-licensed EIAs (second-generation EIAs). BBI provided comprehensive data ...
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Evaluation of erythrocyte autoantibodies with flow cytometric phagocytosis assay
... monolayer assay using live monocytes is regarded as highly useful in determining the clinical significance of antibodies by observing phagocytosis of sensitized RBCs [3, 13, ...
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