Defective interfering particles
Evolution of Poliovirus Defective Interfering Particles Expressing Gaussia Luciferase
... Characterization of defective interfering particles obtained during passaging of PV-GLuc on HeLa cells. To determine how PV-GLuc genomes were altered to lead to the fluctuation of the GLuc signal, we ...
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Host function-dependent induction of defective interfering particles of vesicular stomatitis virus.
... infection prevented the induction of defective interfering particles of vesicular stomatitis virus, which had been cloned and propagated in cells pretreated with.. actinomycin D.[r] ...
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Low-Fidelity Polymerases of Alphaviruses Recombine at Higher Rates To Overproduce Defective Interfering Particles
... of defective interfering particles (DIs) that also attenuate ...that defective interference resulting from higher recombination rates may be more detrimental to RNA virus mutators than the ...
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High-Throughput Single-Cell Kinetics of Virus Infections in the Presence of Defective Interfering Particles
... ABSTRACT Defective interfering particles (DIPs) are virus mutants that lack essential genes for growth. In coinfections with helper virus, the diversion of viral proteins to the replication and ...
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Collective Viral Spread Mediated by Virion Aggregates Promotes the Evolution of Defective Interfering Particles
... Here, we examine the effects of virion aggregation over several infection cycles. Flow cy- tometry, deep sequencing, infectivity assays, reverse transcription-quantitative PCR, and electron microscopy revealed that ...
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Generation of defective interfering particles of Semliki Forest virus in a clone of Aedes albopictus (mosquito) cells.
... cell cultures. Defective-interfering particles of Sem- liki Forest virus generated in BHK cells do not interfere with viral RNA synthesis in Aedes albopictus cells. Evidence for the synt[r] ...
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Structure and genetic complexity of the genomes of herpesvirus defective-interfering particles associated with oncogenic transformation and persistent infection.
... Coestab- lishment of persistent infection and oncogenic transformation of hamster embryo cells by equine herpesvirus type 1 preparations enriched for defective interfering particles.- J.[r] ...
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Dynamics of Biologically Active Subpopulations of Influenza Virus: Plaque-Forming, Noninfectious Cell-Killing, and Defective Interfering Particles
... cell-killing particles (niCKP), were compared to defective interfering particles ...of particles appeared in large numbers (>5 ⴛ 10 8 /ml), and the plaque-forming particle (PFP) titer ...
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A population of bang-bang switches of defective interfering particles makes within-host dynamics of dengue virus controllable.
... excess defective interfering particles (DI ...DI particles do not stop ...DI particles appears as a potential intervention strategy to attenuate the patient viraemia ...
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Defective interfering particles of parvovirus H-1.
... The variations in size due to the variable number of additions and deletions in the right half of the molecule caused the HindIII 51/100 fragments of the DI-wt and DI-wt-tsl4 preparation[r] ...
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In vitro construction of poliovirus defective interfering particles.
... To construct poliovirus defective interfering (DI) particles in vitro, we synthesized an RNA from a cloned poliovirus cDNA, pSM1(T7)1, which carried a deletion in the genome region corre[r] ...
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Guanidine-resistant defective interfering particles of poliovirus.
... protein can affect the guanidine sensitivity of the virus, then passage of DI particles together with complete virions in the presence of guanidine.. should lead to selection for DI part[r] ...
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Defective Interfering Particles of Poliovirus IV. Mechanisms of Enrichment
... Hyperenrichment was observed in labeled virus produced shortly after addition of labeled uridine, in the progeny from infected cells which were shifted to temperatures below 37 C at 150 [r] ...
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Induction and biological properties of defective interfering particles of rabies virus.
... DI particles ot Flury LEP (low egg passage) strain of VSV (Indiana serotype) (1) were prepared rabies virus isolated from BHK-infected cells in monolayer cultures of BHK cells as previou[r] ...
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Defective Interfering Particles of Poliovirus I. Isolation and Physical Properties
... A parallel preparation of labeled double-stranded RNA prepared from 8 X 107 cells and labeled with 400 MtCi of 3H-uridine yielded 2.5 X 105 counts per min of 3H-uridine-labeled, double-s[r] ...
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Interference among defective interfering particles of vesicular stomatitis virus.
... ,ll RNA was infected cells suggests that some L mRNA was In RNA pre- not terminated, but contained run-through se- particles, as quences with 3' ends identical to those of the cellular R[r] ...
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Structure of the intracellular defective viral RNAs of defective interfering particles of mouse hepatitis virus.
... Previously it was shown that the DI particles obtained from the serial undiluted passages of MHV-JHM contain an RNA species slightly smaller than the genomic RNA of standard virus (18)..[r] ...
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Cytopathogenicity of classical swine fever virus caused by defective interfering particles.
... The CSFV DI particles are derived from three different origins. Since for all three the same genome structure was found, the possibility of one DI particle contaminating the other virus stocks had to be excluded. ...
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Defective interfering particles of parainfluenza virus subtype 5 and interferon induction
... This correlation between high DI levels and GFP+ve cells in which the IFN-β promoter has been activated, supports the conclusion that it is the DI PAMPs generated by errors in the repl[r] ...
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Continuing coevolution of virus and defective interfering particles and of viral genome sequences during undiluted passages: virus mutants exhibiting nearly complete resistance to formerly dominant defective interfering particles.
... Nucleotide sequence of N gene, and inferred amino acid sequence, of tsG3l with the location of amino acid changes seen in the following Sdi- mutants: p12 (clone 3), p171, p200, p223, p25[r] ...
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