denaturing gradient gel electrophoresis
Denaturing Gradient Gel Electrophoresis (DGGE)
... information Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method that separates polymerase chain reaction (PCR)-generated DNA ...agarose gel ...
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Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles
... Received 1 October 2004/Returned for modification 29 January 2005/Accepted 16 May 2005 Studies using molecular techniques have demonstrated that a culture-based approach can severely under- estimate the bacterial ...
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Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology
... of denaturing gradient gel electrophoresis (DGGE) as a tool for the detection of clinically relevant species and to compare the results of detection by DGGE with those by PCR and ...
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Identification of the multiple beta thalassemia mutations by denaturing gradient gel electrophoresis
... used denaturing gradient gel electrophoresis to detect the beta-thalassemia mutations in the Chinese ...that denaturing gradient gel electrophoresis offers a ...
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Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities
... molecules, denaturing gradient gel electrophoresis (DGGE) or temperature gradient gel electrophoresis (TGGE), are the most widely ...
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Microbial profiling of dental caries and periodontitis patients using denaturing gradient gel electrophoresis
... Accepted 08 February, 2012 Oral cavity contains a diverse group of microorganisms which changes from normal to diseased condition like dental caries and periodontitis. It is hard to depict the complete picture of ...
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Studies of the Diversity of Lactobacillus spp. in Fecal Samples Using PCR and Denaturing Gradient Gel Electrophoresis
... Because of its simplicity, PCR is a popular technique with a wide range of applications, and it is a powerful tool because it is easy to perform, very specific and extremely efficient. Since PCR is a rapid technique, ...
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Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil
... by denaturing gradient gel electrophoresis (DGGE) was developed and used to monitor community shifts in a polycyclic aromatic hydrocarbon (PAH) polluted ...
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Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
... PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting was first used for profiling complex microbial populations by Muyzer et ...
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Distribution of eukaryotic picoplankton assemblages across hydrographic fronts in the Southern Ocean, studied by denaturing gradient gel electrophoresis
... Departament de Biologia Marina i Oceanografia, Institut de Cie`ncies del Mar (CMIMA, CSIC), Passeig Marı´tim de la Barceloneta 37-49, E-08003 Barcelona, Catalonia, Spain Abstract We used a molecular fingerprinting ...
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High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing
... PCR-denaturing gradient gel electrophoresis and ...multiplex denaturing gradient gel electrophoresis, pyrosequenc- ing, and a ...
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Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR
... PCR-denaturing gradient gel electrophoresis (DGGE) with universal primers targeting the V3 region of the 16S rRNA gene was employed to characterize the overall COPD patient sputum microbiota ...
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Denaturing gradient gel electrophoresis analysis of archaeal and bacterial populations in a submerged anaerobic membrane bioreactor treating landfill leachate at low temperatures
... Mosey, F. E. (1983) Mathematical modelling of the anaerobic digestion process: regulatory mechanisms for the formation of short-chain volatile acids from glucose. Water Sci. and Technol., 15, 209. Muyzer, G., de Waal, E. ...
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Use of 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis for Analysis of the Microfloras of Healing and Nonhealing Chronic Venous Leg Ulcers
... Wales College of Medicine, Cardiff CF14 4XY, United Kingdom Received 27 June 2003/Returned for modification 19 September 2003/Accepted 7 May 2004 The bacterial microfloras of 8 healing and 10 nonhealing chronic venous ...
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Characterization of Microbial Populations in Litopenaeus vannamei (Boone, 1931) Based Biofloc System Using Denaturing Gradient Gel Electrophoresis Profiles of 16s rRNA
... on electrophoresis of PCR- amplified 16S rDNA fragments in polyacrylamide gels containing a linearly increasing gradient of ...In denaturing gradient gel electrophoresis DNA ...
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Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis (PCR DGGE) Profile of Bacterial Community from Agricultural Soils in Bauchi, North East Nigeria
... Abstract The population dynamics of bacterial community was investigated in three Agricultural soils, designated as Loamy sand (A), Peaty coarse (B) and Loamy coarse sand (C) in North-East, Nigeria. The soil chemical ...
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DIRECT LYSIS GLASS MILK METHOD OF GENOMIC DNA EXTRACTION REVEALS GREATER ARCHAEAL DIVERSITY IN ANAEROBIC BIODIGESTER SLURRY AS ASSESSED THROUGH DENATURING GRADIENT GEL ELECTROPHORESIS
... DNA extraction from anaerobic biodigester slurry is a critical step in all phylogenetic and metagenomic approaches to characterize highly diverse biodigester ecosystem, but little is known about the efficiency of ...
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A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE
... acctt Denaturing high performance liquid chromatography is a relatively new method by which heteroduplex structures formed during the PCR amplification of heterozygote samples can be rapidly ...by ...
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PCR-Denaturing gradient gel electrophoresis profiling of inter- and intraspecies 18S rRNA gene sequence heterogeneity is an accurate and sensitive method to assess species diversity of arbuscular mycorrhizal fungi of the genus Gigaspora
... PCR-denaturing gradient gel electrophoresis (DGGE) revealed that the V3-V4 region of the 18S rRNA gene contained insufficient variation to discriminate between different Gigaspora ...
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Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection
... r denaturing gra dien t, and e le c tro p h o re tic conditions fo r its analysis can be inferred to allow the detection of all single base changes at any position in the ...2.2. Denaturing gradient ...
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