Denaturing gradient gel electrophoresis analyses
Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles
... In conclusion, we have used a number of different tech- niques to analyze DGGE banding profiles, each of which an- swer different and progressively more complex questions. The two analyses used first, i.e., the ...
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Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection
... 4.3.1 Choosing a detection strategy Table 4.2 sum m arises the advantages and disadvantages observed for the most commonly used screening methods (see also section 1.2.3.3). As reviewed in Grompe, 1993 the requirements ...
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Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities
... of denaturing gels can result in significant gel-to-gel variations that can make the comparison of large sample sets ...TGGE analyses are faster, with an electrophoresis step less than ...
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Diversity of Hepatitis C Virus Quasispecies Evaluated by Denaturing Gradient Gel Electrophoresis
... per gel, two gels per run) can be screened during a single ...differing gel migratory positions are rapidly identified, to be processed further for nucleotide se- quencing analyses if ...out ...
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Evaluation of Denaturing Gradient Gel Electrophoresis (DGGE) for the Determination of Marine Phytoplankton Genetic Diversity
... establishment of pure cultures of the species to be studied. These types of analyses are suitable for comparison of strains of the same species, different species within the same genus, or at other similar closely ...
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Assessment of the genotypic diversity of antibiotic-producing Pseudomonas species in the rhizosphere by denaturing gradient gel electrophoresis
... samples. Denaturing gra- dient gel electrophoresis (DGGE) of 350-bp fragments of phlD, a key gene involved in DAPG biosynthesis, allowed discrimination between genotypically different phlD ⴙ ...
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Denaturing Gradient Gel Electrophoresis of PCR Amplified gki Genes: a New Technique for Tracking Streptococci
... ent gel electrophoresis (DGGE) technique consisting of spe- cific PCR primers, targeting the household gene glucose kinase (gki), used in combination with DGGE to discriminate be- tween different ...
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Characterization of Microbial Populations in Litopenaeus vannamei (Boone, 1931) Based Biofloc System Using Denaturing Gradient Gel Electrophoresis Profiles of 16s rRNA
... the gel after the silver ...sequence analyses, totally Fifteen isolates were found in biofloc and the bacteria containing the DNA fragments of Band 5, 8 & 9 were not found in the NCBI database so it ...
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DIRECT LYSIS GLASS MILK METHOD OF GENOMIC DNA EXTRACTION REVEALS GREATER ARCHAEAL DIVERSITY IN ANAEROBIC BIODIGESTER SLURRY AS ASSESSED THROUGH DENATURING GRADIENT GEL ELECTROPHORESIS
... DNA extraction from anaerobic biodigester slurry is a critical step in all phylogenetic and metagenomic approaches to characterize highly diverse biodigester ecosystem, but little is known about the efficiency of ...
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Temperature Gradient Gel Electrophoresis as a Valuable Accessory Tool for Assessment of Dysbiosis in Crohn’s Disease
... to electrophoresis in dena- turing gradient gels, where they are identified by bands of characteristic ...the gel, purified and ...amplicon electrophoresis in denaturing gradient ...
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PCR-Denaturing gradient gel electrophoresis profiling of inter- and intraspecies 18S rRNA gene sequence heterogeneity is an accurate and sensitive method to assess species diversity of arbuscular mycorrhizal fungi of the genus Gigaspora
... Sequence analysis of PCR-DGGE banding patterns. To gain further insight into the nature of the intra- and interspecies heterogeneity detected, sequence information was obtained for the region analyzed by PCR-DGGE for ...
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Denaturing Gradient Gel Electrophoresis (DGGE)
... information Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method that separates polymerase chain reaction (PCR)-generated DNA ...agarose gel ...
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Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology
... of denaturing gradient gel electrophoresis (DGGE) as a tool for the detection of clinically relevant species and to compare the results of detection by DGGE with those by PCR and ...
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Identification of the multiple beta thalassemia mutations by denaturing gradient gel electrophoresis
... used denaturing gradient gel electrophoresis to detect the beta-thalassemia mutations in the Chinese ...that denaturing gradient gel electrophoresis offers a ...
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Microbial profiling of dental caries and periodontitis patients using denaturing gradient gel electrophoresis
... Accepted 08 February, 2012 Oral cavity contains a diverse group of microorganisms which changes from normal to diseased condition like dental caries and periodontitis. It is hard to depict the complete picture of ...
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Studies of the Diversity of Lactobacillus spp. in Fecal Samples Using PCR and Denaturing Gradient Gel Electrophoresis
... Because of its simplicity, PCR is a popular technique with a wide range of applications, and it is a powerful tool because it is easy to perform, very specific and extremely efficient. Since PCR is a rapid technique, ...
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Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil
... by denaturing gradient gel electrophoresis (DGGE) was developed and used to monitor community shifts in a polycyclic aromatic hydrocarbon (PAH) polluted ...
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Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
... PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting was first used for profiling complex microbial populations by Muyzer et ...
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Distribution of eukaryotic picoplankton assemblages across hydrographic fronts in the Southern Ocean, studied by denaturing gradient gel electrophoresis
... Departament de Biologia Marina i Oceanografia, Institut de Cie`ncies del Mar (CMIMA, CSIC), Passeig Marı´tim de la Barceloneta 37-49, E-08003 Barcelona, Catalonia, Spain Abstract We used a molecular fingerprinting ...
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High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing
... PCR-denaturing gradient gel electrophoresis and ...multiplex denaturing gradient gel electrophoresis, pyrosequenc- ing, and a ...
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