Denaturing gradient gel electrophoresis analysis
Denaturing gradient gel electrophoresis analysis of archaeal and bacterial populations in a submerged anaerobic membrane bioreactor treating landfill leachate at low temperatures
... Mosey, F. E. (1983) Mathematical modelling of the anaerobic digestion process: regulatory mechanisms for the formation of short-chain volatile acids from glucose. Water Sci. and Technol., 15, 209. Muyzer, G., de Waal, E. ...
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Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities
... molecules, denaturing gradient gel electrophoresis (DGGE) or temperature gradient gel electrophoresis (TGGE), are the most widely ...the analysis of microbial ...
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Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles
... cluster analysis, both provide useful data but do not utilize all avail- able ...Cluster analysis, on the other hand, attempts to differentiate between cohorts that contain large differences within the ...
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Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology
... Online analysis at the RDPII database revealed 42 positive matches for probe Aa502, compared to only 8 positive matches for the reverse primer and 15 positive matches for the forward ...
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Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection
... The a sso cia tio n w ith p a rtic u la r m utations and d iffe re n t m utant haplotypes suggests that there has been m ultiple fo u n d in g p o p u la tio n s for the PAH m u ta tio n s. These observations suggest ...
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Diversity of Hepatitis C Virus Quasispecies Evaluated by Denaturing Gradient Gel Electrophoresis
... Lichfield, Staffordshire, United Kingdom), and bands were visualized by UV transillumination. Sequence analysis. Following DGGE, the migration positions of the various PCR products were first inspected. For cloned ...
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Evaluation of Denaturing Gradient Gel Electrophoresis (DGGE) for the Determination of Marine Phytoplankton Genetic Diversity
... optimize gradient gel electrophoresis (DGGE) protocols for determination of marine eukaryotic phytoplankton genetic ...species. Analysis of natural phytoplankton samples from six different ...
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Microbial profiling of dental caries and periodontitis patients using denaturing gradient gel electrophoresis
... index analysis of dental caries, periodontitis and normal ...microecological analysis of oral flora studied by PCR-DGGE method in combination with cloning and ...
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Assessment of the genotypic diversity of antibiotic-producing Pseudomonas species in the rhizosphere by denaturing gradient gel electrophoresis
... samples. Denaturing gra- dient gel electrophoresis (DGGE) of 350-bp fragments of phlD, a key gene involved in DAPG biosynthesis, allowed discrimination between genotypically different phlD ⴙ ...
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Denaturing Gradient Gel Electrophoresis of PCR Amplified gki Genes: a New Technique for Tracking Streptococci
... PCR analysis was performed using DNA of the following 25 previously isolated bacterial species: Clostridium histolyticum, Clostridium innocuum, Clostridium perfringens, Clostridium difficile, Clostridium ...
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Studies of the Diversity of Lactobacillus spp. in Fecal Samples Using PCR and Denaturing Gradient Gel Electrophoresis
... for analysis of bacterial compositions of microbial communities, where in a typical experiment DNA from the whole community is isolated from the habitat of ...called denaturing gradient gel ...
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Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil
... Sequence analysis of excised band con firmed that the semi-nested PCR protocol produced only amplicons from ciliates thus satisfying one fundamental condition required for environmental diagnostic screening tools, ...
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Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
... correspondence analysis (CCA) revealed that temperature played a key role in the structure of the eukaryotic plankton community in both ponds, but the nutrient concentration did not affect ...
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Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR
... amide gel under UV illumination. The gel fragments were washed once in 200 μl of sterile deionized water and kept in 50 μl of sterile water overnight at 4°C for ...extracted gel mix was heated at ...
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Use of 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis for Analysis of the Microfloras of Healing and Nonhealing Chronic Venous Leg Ulcers
... molecular analysis of the human mi- croflora need not be limited to small numbers of samples as would be the case for the cloning and sequencing ...microbial analysis of clinical specimens and the thorough ...
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High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing
... PCR-DGGE analysis of both the V3 and the V6-7 regions combined with pyrosequencing allowed identification of most helicobacters, but not H. ganmani and H. rodentium, as well as a Helicobacter sp. flexispira-like ...
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Characterization of Microbial Populations in Litopenaeus vannamei (Boone, 1931) Based Biofloc System Using Denaturing Gradient Gel Electrophoresis Profiles of 16s rRNA
... the analysis of the species composition of microbial ...on electrophoresis of PCR- amplified 16S rDNA fragments in polyacrylamide gels containing a linearly increasing gradient of ...In ...
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Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis (PCR DGGE) Profile of Bacterial Community from Agricultural Soils in Bauchi, North East Nigeria
... Abstract The population dynamics of bacterial community was investigated in three Agricultural soils, designated as Loamy sand (A), Peaty coarse (B) and Loamy coarse sand (C) in North-East, Nigeria. The soil chemical ...
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DIRECT LYSIS GLASS MILK METHOD OF GENOMIC DNA EXTRACTION REVEALS GREATER ARCHAEAL DIVERSITY IN ANAEROBIC BIODIGESTER SLURRY AS ASSESSED THROUGH DENATURING GRADIENT GEL ELECTROPHORESIS
... meta- analysis results obtained in different ...PCR- Denaturing Gradient Gel Electrophoresis ...on analysis of PCR-DGGE banding pattern of 16S rRNA gene, Shannon- Wiener and ...
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A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE
... 2-D gradient gel electrophoresis but knowledge about the predicted DNA melting temperature is ...(SNP) analysis by DHPLC, mutation screening by DHPLC should include analysis at lower ...
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