Dense granule
Aspartyl Protease 5 Matures Dense Granule Proteins That Reside at the Host Parasite Interface in Toxoplasma gondii
... (dense granule protein kinases) annotated as ROP21 and ROP27 ...ASP5-cleaved dense granule proteins, including GRA16 and MYR1, despite parasites lacking both of these proteins displaying a ...
21
Development of an Immunochromatographic Assay Based on Dense Granule Protein 7 for Serological Detection of Toxoplasma gondii Infection
... Dense granule antigen proteins derived from Toxoplasma gondii (TgGRAs) are potential antigens for the development of diag- nostic tools. TgGRA7 and TgGRA14 were detected in the peritoneal fluid of T. ...
6
Toxoplasma gondii Parasitophorous Vacuole Membrane Associated Dense Granule Proteins Orchestrate Chronic Infection and GRA12 Underpins Resistance to Host Gamma Interferon
... ABSTRACT Toxoplasma gondii evades host immunity to establish a chronic infec- tion. Here, we assessed the role of parasitophorous vacuole (PV) membrane (PVM)- and intravacuolar network (IVN) membrane-localized ...
20
Three Toxoplasma gondii Dense Granule Proteins Are Required for Induction of Lewis Rat Macrophage Pyroptosis
... vacuole-localized dense granule proteins, GRA35, GRA42, and GRA43, that are individually required for induction of Lewis rat macrophage ...Toxoplasma dense granule proteins play a critical ...
20
Toxoplasma gondii Intravacuolar-Network-Associated Dense Granule Proteins Regulate Maturation of the Cyst Matrix and Cyst Wall
... intravacuolar-network-associated dense granule (GRA) pro- teins GRA1, GRA2, GRA4, GRA6, GRA9, and GRA12 during cyst development in vitro after differentiation of the tachyzoite-stage parasitophorous ...
25
Transcriptomic analysis reveals Toxoplasma gondii strain specific differences in host cell response to dense granule protein GRA15
... Abstract Growth and replication of the protozoan parasite Toxoplasma gondii within host cell entail the production of several effector proteins, which the parasite exploits for counteracting the host’s immune response. ...
24
The tandemly repeated NTPase (NTPDase) from Neospora caninum is a canonical dense granule protein whose RNA expression, protein secretion and phosphorylation coincides with the tachyzoite egress
... the dense granules of intracellular tachyzoites employing a specific α-NcNTPase ...the dense granules, was secreted into the tachyzoite PV, and, similar to NcGRA7, remained there until parasites under- went ...
15
Toxoplasma gondii Parasitophorous Vacuole Membrane-Associated Dense Granule Proteins Regulate Maturation of the Cyst Wall
... Tachyzoite-stage parasites actively penetrate host cells through self-driven motility, and during invagination, the parasite hijacks lipids present in the host cell plasma membrane (6) to establish an intracellular ...
17
Evaluation of Recombinant Dense Granule Antigen 7 (GRA7) of Toxoplasma gondii for Detection of Immunoglobulin G Antibodies and Analysis of a Major Antigenic Domain
... Dense granule protein 7 (GRA7) of Toxoplasma gondii was expressed in Escherichia coli as a fusion protein. The leader peptide contained a 25-amino-acid mouse tumor necrosis factor fragment and six histidyl ...
6
Toxoplasma gondii dense granule protein 15 induces apoptosis in choriocarcinoma JEG 3 cells through endoplasmic reticulum stress
... GRA15: dense granule protein 15; ROP16: rhoptry protein 16; ERS: endoplasmic reticulum stress; STS: staurosporine; TM: tunicamycin; ATF: activating transcription factor; GRP78: 78-kDa glucose-regulated ...
10
Immunization with Oligomannose-Coated Liposome-Entrapped Dense Granule Protein 7 Protects Dams and Offspring from Neospora caninum Infection in Mice
... caninum dense granule protein 7 (NcGRA7) was detected in aborting than in non- aborting cows and heifers, while levels of specific antibodies against parasite surface proteins NcSAG1 and NcSRS2 exhib- ited ...
6
In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis
... In vivo biotinylation of the PV using GRA proteins as bait. To identify a large number of vacuolar resident proteins, we adapted the BioID approach using a secreted GRA protein as bait. To do this, we created a fusion of ...
15
Enzyme-Linked Immunosorbent Assays Using the Recombinant Dense Granule Antigens GRA6 and GRA1 of Toxoplasma gondii for Detection of Immunoglobulin G Antibodies
... much more variable. Such individual immune response could also explain why two sera found negative in the GRA6-Nt ELISA were found to be positive in the GRA1 ELISA. An- other possible source of difference in the ...
5
Bacterial Production of Dense Granule Antigen GRA8 of Toxoplasma gondii
... The dense granule protein GRA2, a new marker for the serodiagnosis of acute Toxoplasma infection: comparison of sera collected in both France and Iran from pregnant ...
7
Translocation of Dense Granule Effectors across the Parasitophorous Vacuole Membrane in Toxoplasma-Infected Cells Requires the Activity of ROP17, a Rhoptry Protein Kinase
... ABSTRACT Toxoplasma gondii tachyzoites co-opt host cell functions through intro- duction of a large set of rhoptry- and dense granule-derived effector proteins. These effectors reach the host cytosol ...
15
Enzyme-Linked Immunosorbent Assays Based on Neospora caninum Dense Granule Protein 7 and Profilin for Estimating the Stage of Neosporosis
... To detect N. caninum infection, many serological diagnostic methods, such as the indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA), have been devel- oped (9). There is accumulating ...
7
Agonist and antagonist effects of diadenosine tetraphosphate, a platelet dense granule constituent, on platelet P2Y1, P2Y12 and P2X1 receptors
... We demonstrate that Ap4A, a known constituent of platelet dense granules, is: a an antagonist of platelet P2Y1 and P2Y12 receptors, where it inhibits the effects of the agonist ADP, b an[r] ...
19
Use of Dense Granule Antigen GRA6 in an Immunoglobulin G Avidity Test To Exclude Acute Toxoplasma gondii Infection during Pregnancy
... serum samples obtained from pregnant women with clinical signs of acute toxoplasmosis (group 2) and with serologic pro- files suggesting acute infection (IgG positive, IgM positive, and [r] ...
7
Role of Pikfyve in Platelet Lysosomal Homeostasis
... granules, dense granules, and lysosomes - that derive from the endolysosomal ...alpha granule and dense granule contents, they develop defective maturation and excessive storage of lysosomal ...
122
Serodiagnosis of Recently Acquired Toxoplasma gondii Infection with a Recombinant Antigen
... the dense granule antigen Gra6, Redlich and Muller applied a cutoff value to their ELISA results and found that 89% of sera from patients with acute toxoplasmosis and only ...
6