Determining the viral DVETNPGP 2A consensus sequence
Correlation between Mutations in the Interferon Sensitivity Determining Region of NS5A Protein and Viral Load of Hepatitis C Virus Subtypes 1b, 1c, and 2a
... [Fig. 2A]) had five mutations in the ISDR and a total of 11 muta- tions in the PKR-binding region and yet showed a high virus ...a sequence identical with phosphorylation sites of PKR and eIF-2␣ and that E2 ...
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Mutagenesis-Induced, Large Fitness Variations with an Invariant Arenavirus Consensus Genomic Nucleotide Sequence
... consensus sequence. Multiple molecular clones were analyzed to define a sequence at nucleotides 470 to 550 within the intergenic region of genomic segment ...hypermutated viral sequences in ...
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Development of global consensus sequence of HCV glycoproteins involved in viral entry
... in viral entry by interacting with an extracellular loop of hu- man CD81, scavenger receptor class B type 1 (SRB-1), high density lipoprotein (HDL) binding molecule and mannose binding proteins (DC-SIGN and ...
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Rotavirus protein NSP3 (NS34) is bound to the 3' end consensus sequence of viral mRNAs in infected cells.
... In this report, we showed that in infected cells, the nonstructural viral protein NSP3 is bound to the 3' end of rotavirus mRNA and protects the whole 3' end consensus sequence from RNas[r] ...
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Effect of oligonucleotide primers in determining viral variability within hosts
... on viral genetic variability at the intrapatient ...that sequence sampling from a single source varied considerably when two slightly different sets of primers were used in the PCR amplification: both sets ...
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Identification of a Novel Consensus Sequence at the Cleavage Site of the Lassa Virus Glycoprotein
... tetrapeptide sequence RRLL 259 , which is conserved in all Lassa virus isolates published to ...the consensus sequence recognized by a novel class of cellular endoproteases which have so far not been ...
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Viral Sequence Evolution in Acute Hepatitis C Virus Infection
... between viral persistence and development of viral escape mutations has been ...HCV sequence evolution, we sequenced the entire HCV genome longitudinally in four subjects monitored for up to 30 ...
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MosaicSolver : a tool for determining recombinants of viral genomes from pileup data
... n consensus viral genome segments), that selection of the same endpoint in each interval represents a parent virus and that a segment pileup proportion can be represented by a point in the as- sociated ...
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Nucleotide Sequence Based Analysis for Determining the Molecular Epidemiology of Penicillium marneffei
... marneffei. In these two isolates the first eight amino acids QALQKGIQ observed in 22 other isolates are replaced by an insertion of the amino acid motif KAIQDGIN (Table 5, Mp1 type 6). In addition, the MP1 nucleotide ...
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Chronic hepatitis B and viral genotype: the clinical significance of determining HBV genotypes
... Depending upon the HBV genotype, these BCP mutations may be found alone or in combination with precore stop codon mutations and the presence of combined mutations may have a direct effect on response to therapy. For ...
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Effect of Intragenic Rearrangement and Changes in the 3′ Consensus Sequence on NSP1 Expression and Rotavirus Replication
... ⴕ consensus sequence for RNA replica- tion and gene ...3⬘-terminal sequence of the gene 5 dsRNAs of all isolates of SA11 rota- viruses was UGAACC and not the expected consensus se- quence ...
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Determining the Cellular Diversity of Hepatitis C Virus Quasispecies by Single-Cell Viral Sequencing
... a sequence within a quasispecies is known to reflect its relative fitness ...encountered sequence and displayed the greatest replicative fitness among the ...the viral loads for the SC populations ...
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Tools for probing 2A sequence space
... of 2A was achieved. This may be because, as with FMDV 2A, eEF-2 promotes translocation of the prolyl-tRNA from the A to the P site thus allowing synthesis of the downstream ...
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The use of viral 2A sequence for the simultaneous over expression of both the vgf gene and enhanced green fluorescent protein eGFP
... The AAV vector (rAAV-VGF) was then produced from this plas- mid vector and infused into the hypothalamus of Siberian hamsters and mice to confirm the effectiveness of the viral 2A sequence in vivo. We ...
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Mutagenic analysis of the coronavirus intergenic consensus sequence.
... pentanucleotide repeats at the 5'-end genomic leader se- quence and at the intergenic region are the same sequence, the precise leader-body fusion site on MHV mRNA has not been identifie[r] ...
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The role of conflict in determining consensus on quality in Wikipedia articles
... Wikipedia – “the free encyclopedia that anyone can edit” [37] – is often the subject of popular debates about the quality of information that can be produced by just “anyone,” rather than the traditional expert authors ...
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On the effective depth of viral sequence data.
... Genome sequence data are of great value in describing evolutionary processes in viral populations. However, in such stud- ies, the extent to which data accurately describes the viral population is a ...
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Aichi Virus 2A Protein Is Involved in Viral RNA Replication
... virus 2A protein is not a protease, unlike many other picornavirus 2A proteins, and it is related to a cellular protein, ...two 2A mutants in Vero cells and a cell-free translation/replication ...
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Use of the viral 2A peptide for bicistronic expression in transgenic mice
... test 2A function in transgenic mice and uncover any possible toxicity of widespread expression of the 2A peptide, we made a bicistronic reporter construct containing the coding sequence for a ...
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5′ Nucleotide Sequence of Sindbis Viral RNA
... A preparation of 32P-labeled Sind- bis viral RNA was treated with pancreatic RNase, the digest was resolved by DEAE-cel- lulose chromatography (Fig. 6), and each indi- cated area of the [r] ...
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