diamidino-2-phenylindole
Electrophoretic Karyotypes and Genome Sizing of the Pathogenic Fungus Paracoccidioides brasiliensis
... Here we present the karyotype analysis and genome sizing of Paracoccidioides brasiliensis, a pathogen refrac- tory to conventional genetic analysis. We have established pulsed-field gel electrophoresis (PFGE) conditions ...
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Modulatory effects of vagal stimulation on neurophysiological parameters and the cellular immune response in the rat brain during systemic inflammation
... for 2 h at room temperature with the secondary antibodies diluted in blocking solution (Alexa488-conju- gated anti-rabbit IgG, dilution 1:500, AZA21206, MoBiTec GmbH, Göttingen, Germany; Cy3-conjugated anti-goat, ...
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ADAM 17 is expressed on rheumatoid arthritis fibroblast like synoviocytes and regulates proinflammatory mediator expression and monocyte adhesion
... For analysis of ADAM-17 expression in RA STs and RA FLSs, rabbit anti-ADAM-17 was used as the primary antibody. (High-activity STs were selected from RA STs using hematoxylin and eosin stain.) (To measure the secretion ...
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Neospora caninum Recruits Host Cell Structures to Its Parasitophorous Vacuole and Salvages Lipids from Organelles
... As in T. gondii (RH)-infected cells, many host mitochondria gath- ered around the N. caninum PV, often in multiple layers (Fig. 2C, panels a and b compared to panel e). Observations at high mag- nification show a close ...
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Cryoinjury-induced acute myocardial infarction model and ameroid constrictor-induced ischemic heart disease model in adult micro-mini pigs for preclinical studies
... liquid nitrogen. Cryosections were stained with primary antibodies against a cardiomyocyte specific marker (tropo- nin-T (Abcam, Cambridge, UK)), an endothelial cell (EC) marker (von Willebrand factor (vWF) (Abcam)), and ...
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Glial cell line-derived neurotrophic factor gene delivery via a polyethylene imine grafted chitosan carrier
... ,6-diamidino-2- phenylindole dihydrochloride (DAPI), fluorescein isothio- cyanate (FITC), and ethidium bromide (EtBr) were provided by Invitrogen (Camarillo, CA, ...
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Fucosyltransferase 1 mediates angiogenesis, cell adhesion and rheumatoid arthritis synovial tissue fibroblast proliferation
... Immunofluorescence staining on RA ST fibroblasts was performed as previously described [25]. To determine if α(1,2)-linked proteins were expressed on RA ST syno- vial fibroblasts, mouse anti-human collagen-1 (Abcam, ...
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Detection of ALK fusion transcripts in FFPE lung cancer samples by NanoString technology
... assay using the commercially available ALK probe (Vysis LSI ALK Dual Color, Break Apart Rearrangement Probe; Abbott Molecular, Abbott Park, IL) as described else- where [18]. The lung adenocarcinoma cell lines H2228 ...
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Identification of a novel autoantibody against self vimentin specific in secondary Sjögren’s syndrome
... 3S-P: Secondary Sjögren ’ s syndrome-associated peptide; AS: Ankylosing spondylitis; BSA: Bovine serum albumin; DAPI: 4 ′ ,6-Diamidino-2-phenylindole; ELISA: Enzyme-linked immunosorbent assay; FITC: ...
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Protective effects of a compound herbal extract (Tong Xin Luo) on free fatty acid induced endothelial injury: Implications of antioxidant system
... 4'6' Diamidino-2-phenylindole dihydrochloride: DAPI; 5'AMP-activated protein kinase: AMPK; Bovine serum albumin: BSA; Coronary artery disease: CAD; Dimethyl sulfoxide: DMSO; Dithiothreitol: DTT; ...
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Near-infrared quantum dots for HER2 localization and imaging of cancer cells
... as well. Previous studies have demonstrated a complete out- line of the cells because the QD-antibody conjugate attaches to the extracellular receptors. However, most of these studies used QD-based immunohistochemistry ...
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A rodent model of anterior ischemic optic neuropathy (AION) based on laser photoactivation of verteporfin
... TdT-mediated dUTP Nick-end labeling (TUNEL) assay On days 1, 4, 7 and/or 14 following laser application, paraffin-embedded retinal tissue sections were deparaffi- nized, rehydrated, fixed with 4% PFA for 15 min at 4 °C ...
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Transforming growth factor beta receptor type III is a tumor promoter in mesenchymal stem like triple negative breast cancer
... Migrations (Costar, #3422, Tewksbury, MA, USA) were conducted by plating 2.5 x 10 4 cells in the top of the trans- well and media with 10% FBS in the bottom of the well to act as a chemoattractant. Cells were fixed in 4% ...
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Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus
... The efficiency of DHBV infection in PDHs was deter- mined by indirect immunofluorescence and Southern blot hybridization. Monolayers of PDHs grown on glass coverslips were fixed directly by adding 4% polyoxymeth- ylene ...
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Localization of Single- and Low-Copy Sequences on Tomato Synaptonemal Complex Spreads Using Fluorescence in Situ Hybridization (FISH)
... Fluorescence in situ hybridization (FISH) is a powerful means by which single- and low-copy DNA sequences can be localized on chromosomes. Compared to the mitotic metaphase chromosomes that are normally used in FISH, ...
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Karyotype analysis of Lablab purpureus (L ) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes
... seven cultivated Vigna species (de A. Bortoleti et al. 2012; She et al. 2015), the four cultivated Phaseo- lus species (Bonifácio et al. 2012), Psophocarpus tetragonolobus (Chaowen et al. 2004), and Glycine max (She et ...
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Diarylamidines: high potency inhibitors of acid- sensing ion channels
... Acid-sensing ion channels (ASICs) are proton-gated cation channels that are predominantly expressed in the nervous system. ASICs are involved in a number of neurological diseases such as pain, ischemic stroke and ...
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Effects of stachydrine on norepinephrine induced neonatal rat cardiac myocytes hypertrophy and intracellular calcium transients
... cAMP and subsequent activation of PKA. PKA in turn phosphorylates several downstream targets which are the key players in cellular calcium handling. Those targets in- clude: L-type Ca 2+ channel, Ryanodine ...
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Prymnesiophytes of New Zealand's coastal waters : taxonomy, physiology and ecology : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Plant Biology and Biotechnology at Massey University
... M 2 R i s a fluorescent dye, o r fluorochrome, which binds to cellulose and other p -linked g l ucans ( H ughs and M c C ul ly, 1 9 7 5 ) ; the m o l ecules also h ave a known affi nity for chiti n ( H a rrington ...
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Humoral response in experimental autoimmune encephalomyelitis targets neural precursor cells in the central nervous system of naive rodents
... For IHC, brain paraffin sections were deparaffinized, hy- drated, rinsed, and then blocked with hydrogen peroxide in methanol. Incubation of sections with blocking buffer (10% fetal bovine serum, FBS; Merck Millipore, ...
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