Digestion with Restriction Endonuclease
Differentiation of Acanthamoeba strains from infected corneas and the environment by using restriction endonuclease digestion of whole cell DNA
... Restriction endonuclease digestion of Acanthamoeba whole-cell DNA was used to study the relationship between 33 morphologically identical strains from keratitis cases (30 strains), conta[r] ...
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Comparison of JC and BK Human Papovaviruses with Simian Virus 40: Restriction Endonuclease Digestion and Gel Electrophoresis of Resultant Fragments
... taken to purify JC virus, and to compare its DNA with that of BK and SV40 viruses by restriction endonuclease digestion and gel elec- trophoresis of resultant fragments, to test.. whethe[r] ...
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COMPARISON OF THE RESTRICTION ENDONUCLEASE DIGESTION PATTERNS OF MITOCHONDRIAL DNA FROM NORMAL AND MALE STERILE CYTOPLASMS OF ZEA MAYS L
... High resolution gel electrophoresis has allowed the assignment of fragment number and molecular weight to EcoRI, Sal1 and PstI restriction fragments of mitochondrial DNA from[r] ...
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Chromosomal DNA restriction endonuclease digestion patterns of beta lactamase producing Enterococcus faecalis isolates collected from a single hospital over a 7 year period
... As shown here and previously (11), most E. faecalis strains have 15 to 20 SmaI chromosomal digestion fragments. None of the b -lac 1 isolates examined in this study had patterns that resembled those of the b -lac ...
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Restriction Endonuclease Analysis Discriminates Bordetella bronchiseptica Isolates
... by restriction enzyme analysis ...Twenty restriction endonucleases were evaluated for use in REA. Digestion of chromosomal DNA with HinfI, followed by submarine electrophoresis in agarose gels and ...
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Endonuclease-Mediated Long PCR and Its Application to Restriction Mapping
... no restriction sites for EcoRI, while STP1 and STP2 both contain EcoRI restriction ...prior restriction digestion with EcoRI, and the enhanced specificity and yield of the STP2 amplification ...
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Rapid in situ generation of DNA restriction endonuclease patterns for Neisseria gonorrhoeae
... Restriction endonuclease (RE) digestion patterns of 26 isolates of Neisseria gonorrhoeae representing different serovars of serogroups Wl, WII, and WIII were generated by agarose pellet [r] ...
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Restriction endonuclease analysis of human and bovine group B streptococci for epidemiologic study
... care unit outbreak isolate, type lb/c (no.. Double digestion of GBS isolates with BgIII and then EcoRI. 437); 2 to 4, type lb/c neonatal intensive care unit outbreak isolates (no. 4 and [r] ...
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Identification of novel restriction endonuclease-like fold families among hypothetical proteins
... RecC RecC functions together with RecB and RecD in a multi- functional enzyme complex to process DNA ends resulting from a double-strand break, leading to repair by homologous recombination. In this process, helicase and ...
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Rapid Typing of Human Adenoviruses by a General PCR Combined with Restriction Endonuclease Analysis
... PCR-RE digestion system to evaluate the ...PCR-RE digestion method is reliable and can be used as a tool for characterization of Ads in samples in daily routine diagnostic ...
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Analysis of Simian Virus 40 DNA with the Restriction Enzyme of Haemophilus aegyptius, Endonuclease Z
... Limited digestion of simian virus 40 (SV40) DNA from both small- and large-.. plaque strains with the restriction endonuclease Z from Haemophilus aegyptius.[r] ...
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Restriction endonuclease analysis of Eikenella corrodens
... Group 1 included 47 epidemiologically independent isolates recovered from dental plaques in periodontally healthy subjects and periodontitis patients and from extraoral infections in dif[r] ...
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Restriction Digestion Protocol Pdf
... given digestion will result in cell biology and visualization, so using a sample! If you use for undergraduate and chemistry, voltage and computational biology is a population and interaction of sequence ...of ...
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Restriction endonuclease analysis of nosocomial isolates of Clostridium difficile
... A total of 110 clinical isolates of Clostridium difficile were analyzed by agarose gel electrophoresis by using both bacterial restriction endonuclease analysis (REA) and plasmid profile[r] ...
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Restriction endonuclease cleavage map of the DNA of JC virus.
... A physical map of the sites cleaved by the following restriction endonucleases was derived for the DNA of JC virus, a human polyomavirus: EcoRI, HpaI, and PstI (one site each); HindII (f[r] ...
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Bio 6 Restriction Enzyme Digestion Lab
... HaeII restriction site can be any purine (A or G) or pyrimidine (C or T), respectively, whereas the third position of the ScrFI restriction site can be any of the four bases (A, C, G or ...given ...
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Bacterial chromosomal restriction endonuclease analysis of the homology of Bacteroides species
... In contrast, bacterial chromosomal restriction endonuclease analysis in conjunction with Southern hybridizations was most effectively used to determine the significance of low levels of [r] ...
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Molecular epidemiology of Legionella species by restriction endonuclease and alloenzyme analysis
... As part of an ongoing investigation into nosocomial Legionella infections at Stanford University Medical Center (SUMC), we applied the technique of restriction endonuclease analysis (REA[r] ...
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Restriction endonuclease analysis of DNA from Chlamydia trachomatis biovars
... isolates generated similar fragment patterns; however, distinct fragments appeared to be unique to both biovars, thus allowing differentiation of these two major groups.. In most cases, [r] ...
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Developing a programmed restriction endonuclease for highly specific DNA cleavage
... REase with 8 bp recognition sites). In principle, this or similar constructs could therefore be used to map chromosomal DNA and to clone very large DNA fragments. Given the fact that TFO can be synthesized that would ...
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