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digital PCR

Applications of Digital PCR for Clinical Microbiology

Applications of Digital PCR for Clinical Microbiology

... of PCR primers and probes must be optimized, the use of an internal control is still recommended, and all assay validation steps must be performed prior to use on clinical ...Quantitative Digital PCR ...

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Quantitative Assessment of Commutability for Clinical Viral Load Testing Using a Digital PCR Based Reference Standard

Quantitative Assessment of Commutability for Clinical Viral Load Testing Using a Digital PCR Based Reference Standard

... spondence analysis prediction ellipses are two statistical methods that are commonly used to evaluate commutability in the litera- ture. These methods have provided useful insights regarding the commutability of several ...

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Detection of Methicillin Resistant Staphylococcus aureus by a Duplex Droplet Digital PCR Assay

Detection of Methicillin Resistant Staphylococcus aureus by a Duplex Droplet Digital PCR Assay

... droplet digital PCR (ddPCR) assay, which is a next-generation emulsion-based endpoint PCR assay for high-precision MRSA ...Quantitative PCR (qPCR) (Roche Light Cycler 480) and ddPCR (Bio-Rad ...

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Non-invasive fetal sex diagnosis in plasma of early weeks pregnants using droplet digital PCR

Non-invasive fetal sex diagnosis in plasma of early weeks pregnants using droplet digital PCR

... ddPCR is a recent technology known for its high sensi- tivity (Pekin et al., 2011). The great sensitivity of the technique is due to the extreme dilution, and hence sub- division of the droplet sample consisting of an ...

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Highly sensitive and quantitative evaluation of the EGFR T790M mutation by nanofluidic digital PCR

Highly sensitive and quantitative evaluation of the EGFR T790M mutation by nanofluidic digital PCR

... and PCR reagents into 12 individual ...of PCR, the number of signals obtained from successfully reacted chambers was counted with the Fluidigm digital PCR analysis software (raw ...

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Quantification of Zoonotic Bacterial Pathogens within Commercial Poultry Processing Water Samples Using Droplet Digital PCR

Quantification of Zoonotic Bacterial Pathogens within Commercial Poultry Processing Water Samples Using Droplet Digital PCR

... generation PCR technology, known as droplet digital PCR (ddPCR) has been devel- oped to be more accurate and sensitive to detect genetic targets than current quantification methods, but this ...

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The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis

... [21]. Digital PCR (dPCR) [22, 23] may offer such an approach through binary count- ing of the number of pathogen genomes present which does not rely on an additional calibration material like qPCR, and has ...

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A comparison of QuantStudio™ 3D Digital PCR and ARMS-PCR for measuring plasma EGFR T790M mutations of NSCLC patients

A comparison of QuantStudio™ 3D Digital PCR and ARMS-PCR for measuring plasma EGFR T790M mutations of NSCLC patients

... 3D Digital PCR with the ARMS- PCR method for detecting T790M ...3D Digital PCR assay was more sensitive and specific than ARMS-PCR for detecting T790M mutation in plasma ...

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Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples

Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples

... quantitative PCR has enabled high-throughput detection and quantification of pathogens; however, its quantitative use is linked to standardized reference materials, and its sensitivity to inhibitors can lead to ...

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Accurate Detection of Methicillin Resistant Staphylococcus aureus in Mixtures by Use of Single Bacterium Duplex Droplet Digital PCR

Accurate Detection of Methicillin Resistant Staphylococcus aureus in Mixtures by Use of Single Bacterium Duplex Droplet Digital PCR

... duplex PCR methods are not able to differentiate MRSA from coex- isting methicillin-susceptible ...droplet digital PCR (ddPCR) with the chimeric lysin ClyH for cell ...

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Comparative Performance of Reagents and Platforms for Quantitation of Cytomegalovirus DNA by Digital PCR

Comparative Performance of Reagents and Platforms for Quantitation of Cytomegalovirus DNA by Digital PCR

... droplet digital PCR (ddPCR) systems were used: the QX200 droplet digital PCR system with an automated droplet generator (Bio-Rad, Pleasanton, CA) and the RainDrop digital PCR ...

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Quantitation of Integrated HIV Provirus by Pulsed Field Gel Electrophoresis and Droplet Digital PCR

Quantitation of Integrated HIV Provirus by Pulsed Field Gel Electrophoresis and Droplet Digital PCR

... droplet digital PCR (ddPCR), which is a sensitive and precise platform for quantifying DNA and RNA sequences of multiple viral pathogens, including HIV and herpesviruses (15, ...

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Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR

Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR

... The seven pSEVA plasmids only differing in the replica- tion system demonstrated different degrees of heteroge- neity in terms of EGFP fluorescence. One explanation for such differences can be the average copy number of ...

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Development and Evaluation of a Next Generation Digital PCR Diagnostic Assay for Ocular Chlamydia trachomatis Infections

Development and Evaluation of a Next Generation Digital PCR Diagnostic Assay for Ocular Chlamydia trachomatis Infections

... of digital PCR make it a process that has clear utility in the field of quantitative infectious disease diagnostics ...early PCR cycles can potentially affect changes in PCR amplification ...

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Reproducibility of Digital PCR Assays for Circulating Tumor DNA Analysis in Advanced Breast Cancer.

Reproducibility of Digital PCR Assays for Circulating Tumor DNA Analysis in Advanced Breast Cancer.

... of digital PCR (dPCR) assays of circulating tumor DNA in a cohort of patients with advanced breast cancer and assessed delayed plasma processing using cell free DNA preservative ...plex digital ...

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Original Article Detection of IDH1 and TERT promoter mutations with droplet digital PCR in diffuse gliomas

Original Article Detection of IDH1 and TERT promoter mutations with droplet digital PCR in diffuse gliomas

... [18] Hindson BJ, Ness KD, Masquelier DA, Belgrad- er P, Heredia NJ, Makarewicz AJ, Bright IJ, Lu- cero MY, Hiddessen AL, Legler TC, Kitano TK, Hodel MR, Petersen JF, Wyatt PW, Steenblock ER, Shah PH, Bousse LJ, Troup CB, ...

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Diagnostic accuracy of droplet digital PCR for detection of EGFR T790M mutation in circulating tumor DNA

Diagnostic accuracy of droplet digital PCR for detection of EGFR T790M mutation in circulating tumor DNA

... We comprehensively searched various online sources includ- ing PubMed, Web of Science, Embase and Cochrane Library up to October 11, 2017, using key words “digital PCR” and “T790M”. The language is limited ...

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Droplet Digital PCR Based Detection of Clarithromycin Resistance in Helicobacter pylori Isolates Reveals Frequent Heteroresistance

Droplet Digital PCR Based Detection of Clarithromycin Resistance in Helicobacter pylori Isolates Reveals Frequent Heteroresistance

... ABSTRACT Chronic infection with Helicobacter pylori causes peptic ulcers and stom- ach cancer in a subset of infected individuals. While standard eradication therapy in- cludes multiple antibiotics, treatment failure due ...

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A Direct Droplet Digital PCR Method for E  coli Host Residual DNA Quantification

A Direct Droplet Digital PCR Method for E coli Host Residual DNA Quantification

... droplet digital PCR (ddPCR) as an alternative method ...the PCR reaction is partitioned into about 20,000 individual nanoliter-sized droplets using micro- ...the PCR is run to endpoint, the ...

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Digital PCR methods improve detection sensitivity and measurement precision of low abundance mtDNA deletions

Digital PCR methods improve detection sensitivity and measurement precision of low abundance mtDNA deletions

... and digital PCR-based methods of mtDNA deletion detection using double-stranded reference templates or biological ...the digital PCR method significantly improved mtDNA deletion detection ...

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