Dihydrofolate reductase
Insights into the role of the junctional region of Plasmodium falciparum dihydrofolate reductase thymidylate synthase
... Malaria remains an important disease in many tropical and sub-tropical countries [1]. The disease has become a global health threat, with over one million deaths annually, mostly children in sub-Saharan Africa [2]. The ...
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Trends in drug resistance codons in Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase genes in Kenyan parasites from 2008 to 2012
... SP resistance is conferred by mutations in Plasmodium falciparum dihydrofolate reductase ( Pfdhfr ) and P. falcip- arum dihydropteroate synthase ( Pfdhps ) genes [8,9], whose enzymes are target for ...
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Multiple origins of resistance conferring mutations in Plasmodium vivax dihydrofolate reductase
... Point mutations in the gene that encodes dihydrofolate reductase (DHFR) confer resistance to pyrimethamine in both P. vivax and P. falciparum, and mutant alleles have been identified in most endemic regions ...
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Island wide diversity in single nucleotide polymorphisms of the Plasmodium vivax dihydrofolate reductase and dihydropteroate synthetase genes in Sri Lanka
... vivax dihydrofolate reductase (Pfdhfr) and dihydropteroate synthetase (Pvdhps) genes cause parasite resistance to the antifolate drug combination, sulphadoxine/ pyrimethamine ...
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Novel Potent Inhibitors of Plasmodium vivax Dihydrofolate Reductase: An in silico Antimalarial Drug Discovery
... the dihydrofolate reductase enzyme that catalyzes the reduction of dihydrofolate to tetrahydrofolate which is required for the purines and pyrimidine ...sapiens dihydrofolate reductase ...
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A STUDY ON DIHYDROFOLATE REDUCTASE AND ITS INHIBITORS: A REVIEW
... Enzyme Dihydrofolate reductase is a relatively soluble water soluble protein with a molecular weight of 18000-20000 Da synthesised on activation of human DHFR located on q22 region of chromosome no 5 ...
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INCREASED DIHYDROFOLATE REDUCTASE SYNTHESIS IN DIPLOCOCCUS PNEUMONIAE FOLLOWING TRANSLATABLE ALTERATION OF THE STRUCTURAL GENE. III. FURTHER EVIDENCE ON THE EXTENT OF GENIC INVOLVEMENT
... Increased rates of dihydrofolate reductase synthesis were determined by mu- tation (amer) at sites distributed throughout a large portion, if not all, of the cor- responding s[r] ...
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Mutational analysis of Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase genes in the interior division of Sabah, Malaysia
... Plasmodium falciparum , which causes the most severe form of malaria, is found mainly in the tropical and subtropical regions including Sabah, Malaysian Borneo. The spread of resistance to anti-malarial drugs poses an ...
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Polymorphism of Plasmodium Falciparum Dihydrofolate Reductase and Dihydropteroate Synthase Genes among Pregnant Women with Falciparum Malaria in Banjar District, South Kalimantan Province, Indonesia
... 27. Mugittu K, Ndejembi M., Malisa A., Lemnge M, Premji Z, Mwita A, Nkya W, Kataraihya J, Abdulla S, Beck HP & Mshinda H (2004) Therapeutic Efficacy of Sulfadoxine- Pyrimethamine and Prevalence of Resistance Markers ...
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HYPERPRODUCTION OF DIHYDROFOLATE REDUCTASE IN DIPLOCOCCUS PNEUMONIAE AFTER MUTATION IN THE STRUCTURAL GENE. EVIDENCE FOR AN EFFECT AT THE LEVEL OF TRANSCRIPTION
... strains: If the effects of the amer structural gene mutations on dihydrofolate reductase synthesis are mediated at the level of transcription, i t should be possible, in th[r] ...
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Bacteriophage T4 Virion Dihydrofolate Reductase: Approaches to Quantitation and Assessment of Function
... Although the enzyme duplicates a preexisting host cell activity, the viral enzyme does participate in the greatly increased rates of DNA precursor synthesis seen in infected cells, for T[r] ...
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Multi-timescale dynamic effects of antifolate binding and mutations in dihydrofolate reductase
... site, thus occluding the cofactor binding pocket. To form the occluded conformation, hydrogen bond interactions between the Met20 loop and the F-G loop are broken, and new interactions with the residues on the G-H loop ...
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Bacteriophage T4 Virion Baseplate Thymidylate Synthetase and Dihydrofolate Reductase
... With regard to phage td it has been found that: i low levels of thymidylate synthetase activity were present in highly purified preparations of T4D ghost particles produced after infecti[r] ...
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Abundant expression of polyomavirus middle T antigen and dihydrofolate reductase in an adenovirus recombinant.
... Two cDNA segments have been inserted into this adenovirus vector and expressed at high levels: one encoding the pymT Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest -p [r] ...
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Bacteriophage T4-coded dihydrofolate reductase: synthesis, turnover, and location of the virion protein.
... Any or all of the following factors might contribute to an explanation of the apparent absence of such bands: i the proteins might be present in such low amounts that our procedures did [r] ...
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Control of synthesis of mRNA's for T4 bacteriophage-specific dihydrofolate reductase and deoxycytidylate hydroxymethylase.
... Effect of DNA-negative and maturation-defective conditions on accumulation of functional messengers for T4 bacteriophage-specific dihy- chloramphenicol and starvation for an essential am[r] ...
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Inactivation of T4D Bacteriophage by Antiserum Against Bacteriophage Dihydrofolate Reductase
... Progress has been made in determining the biological role of the phage tail-plate DFR in phage infection 9, but little is known of the structural details of incorporation of this enzyme [r] ...
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Function of T4D Structural Dihydrofolate Reductase in Bacteriophage Infection
... Although it is not shown in this experiment, Ability of various compounds to inhibit addition of 5 rnM NADPH restored adsorption phage adsorption or relieve ADPR inhibition rates to thos[r] ...
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Geographic distribution of amino acid mutations in DHFR and DHPS in Plasmodium vivax isolates from Lao PDR, India and Colombia
... Molecular determination of anti‑ folate resistance associated point mutations in Plasmodium falciparum dihydrofolate reductase dhfr and dihydropteroate synthetase dhps genes among the fi[r] ...
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Molecular markers for artemisinin and partner drug resistance in natural Plasmodium falciparum populations following increased insecticide treated net coverage along the slope of mount Cameroon: cross-sectional study
... parasite dihydrofolate reductase (dhfr) and dihydpteroate synthetase (dhps) genes that con- fer resistance to pyrimethamine and sulphadoxine respect- ively [26, ...
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